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The aim of the study was to evaluate the influence of large doses of exogenous gonadotrophins on ovarian follicular growth in goats. Folliculogenesis in twelve Polish White Goats was studied in the breeding season. In every goat synchronization of estrus was realized using FGA. In group 1 (6 goats), 1200 iu of PMSG was injected at the day of sponge withdrawal (day 17). In group 2 (6 goats), for four consecutive days from day 15, 40 iu, 30 iu, 20 iu, 10 iu of FSH was injected twice a day. Daily examinations of ovaries were performed from the day when sponges were inserted, to the ovulation in the second day after superovulation estrous cycle. The number of follicles growing during medication using FGA did not differ between groups, and did not differ from observations performed during natural estrous cycle. In ovulation wave of group 1 22.2 (± 3.92) of follicles were growing, and 21 (± 3.95) reached diameter of ³ 5 mm. In ovulation wave of group 2 20.2 (± 2.93) of follicles were growing, and 18.5 (± 2.07) reached a diameter of ³ 5 mm. The differences between groups were insignificant. The ovulation rate in group 1 was 13.2 (± 3.06), and was significantly lower (P £ 0.05) than in group 2, i.e. 17.5 (± 3.67). After ovulation in the animals of group 1, follicular cysts appeared and disturbed folliculogenesis of the next estrous cycle. In the first wave there were more follicles then in the natural cycle, but after the second wave recruitment was stopped. In the ovulation wave less follicles were growing than in the natural cycle, however the ovulation rate was typical. There were larger numbers of growing follicles in the first postovulatory wave in group 2, nevertheless there were no disturbances in the rest of the cycle.
In this study the dynamics of follicular growth during estrous cycle after cloprostenol, FGA and PMSG stimulation in goats was compared. On the day 11 of the estrous cycle Group I of the goats (n = 4) was injected with 0.25 mg of cloprostenol for luteolysis stimulation. The follicles which started to grow after treatment formed the ovulatory wave. Group II of the goats (n = 4) was stimulated for 17 days by intravaginal sponges with FGA. After withdrawal of the sponges goats were injected with a single dose of 500 I.U. of PMSG (day 17). Observation of growing follicles on the ovaries were carried out every day through the two consecutive estrous cycles, and was finished on the day of ovulation at the end of second estrous cycle. No differences were observed in total number (4.25 ± 0.5) and the number of the large follicles ≥ 5 mm (2.75 ± 0.5) of the ovulatory wave after cloprostenol injection in comparison to waves of follicles growing during the luteal phase. In Group II of the goats stimulated with sponges impregnated with FGA the number of growing follicles did not differ from the number of follicles in Group I of goats and goats with a natural estrous cycle. After PMSG injection the number of follicles growing in the ovulatory wave increased rapidly. After synchronised ovulation in Group II of the goats follicular cysts were observed and disturbed folliculogenesis of the subsequent estrous cycle. No second wave of follicles was observed and the number of follicles growing was significantly lower (p ≤ 0.05), even in the ovulatory wave, but the ovulation rate did not change in comparison to the natural estrous cycle.
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