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1

Application of fluorescein-labelled lectins with different glycan-binding specificities to the studies of cellular glycoconjugates in human full-term placenta

100%
Konska G., Zamorska L., Pituch-Noworolska A., Szmaciarz M., Guillot J.
Folia Histochemica et Cytobiologica
|
2003
|
tom 41
|
nr 3
2

Histological, histochemical and immunohistochemical study on the growing oocytes of the abyssal teleost Hoplostethus mediterraneus [V]

75%
Calabro C., Albanese M. P., Bertuccio C., Licata A., Gentile N.
Folia Histochemica et Cytobiologica
|
2008
|
tom 46
|
nr 1
97-102
3

Characterization of bovine serum albumin glycated with glucose, galactose and lactose

63%
Ledesma-Osuna A. I., Ramos-Clamont G., Vazquez-Moreno L.
Acta Biochimica Polonica
|
2008
|
tom 55
|
nr 3
491-497
The non-enzymatic reaction between reducing sugars and proteins, known as glycation, has received increased attention from nutritional and medical research. In addition, there is a large interest in obtaining glycoconjugates of pure well-characterized oligosaccharides for biological research. In this study, glycation of bovine serum albumin (BSA) by d-glucose, d-galactose and d-lactose under dry-heat at 60°C for 30, 60, 120, 180 or 240 min was assessed and the glycated products studied in order to establish their biological recognition by lectins. BSA glycation was monitored using gel electrophoresis, determination of available amino groups and lectin binding assays. The BSA molecular mass increase and glycation sites were investigated by mass spectrometry and through digestion with trypsin and chymotrypsin. Depending on time and type of sugar, differences in BSA conjugation were achieved. Modified BSA revealed reduction of amino groups’ availability and slower migration through SDS/PAGE. d-Galactose was more reactive than d-glucose or d-lactose, leading to the coupling of 10, 3 and 1 sugar residues, respectively, after 120 minutes of reaction. BSA lysines (K) were the preferred modified amino acids; both K256 and K420 appeared the most available for conjugation. Only BSA-lactose showed biological recognition by specific lectins.
4

Diabetic alteration versus postnatal maturation of rat kidney glycoconjugates - comparative detection by lectin probes

63%
Lutsyk A., Ambarova N., Antonyuk V.
Folia Histochemica et Cytobiologica
|
2013
|
tom 51
|
nr 1
5

Applying plant lectins to assay the effect of environmental pollution on the glycosylation of human placenta

63%
Konska G., Pituch-Noworolska A., Kaczmarczyk G., Tyrkalska K., Zamorska L., Guillot J.
Polish Journal of Environmental Studies
|
2005
|
tom 14
|
nr 3
Our study was designed to establish whether air pollution in urbanized industrial centers of southern Poland affects the process of glycosylation in a full-term human placenta. This process of glycosylation was analyzed by the quantitative determination of the binding of WGA and LCA lectins to placental villi. The study was performed on human placentas collected in 1990-91 and 2000-01 in regions of southern Poland differing in their degree of environmental pollution: the highly polluted areas of Upper Silesia and Cracow agglomeration. The Bieszczady area with low pollution was considered the control. The concentrations of nitrogen and sulfur oxides and the concentration of aerosols were used as markers of the degree of air pollution. The direct immunofluorescence reaction of the placenta tissues with fluorescein-labeled (FITC) lectins was used. The staining of the placenta tissues was examined under a fluorescence microscope linked to an analysis system. A microdensytometric method was used to assay the amount of tissue-bound lectins. The results showed no significant effect of the three main air pollutants in the study areas in southern Poland, i.e. nitrogen and sulfur oxides and high level of aerosols, on the structure of WGAand LCA-specific glycoconjugates in human placenta. However, the marked quantitative changes in the degree of lectin binding to placental cellular structures were noted within the last 10-year period in all studied regions.
6
Content available remote

Glycoconjugates with NeuAc-NeuAc-Gal-Glc are more effective at preventing adhesion of Helicobacter pylori to gastric epithelial cells than glycoconjugates with NeuAc-Gal-Glc

63%
Hata Y., Murakami M., Okabe S.
Journal of Physiology and Pharmacology
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2004
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tom 55
|
nr 3
Helicobacter pylori (H. pylori) adheres to human gastric epithelial cells, eliciting various gastroduodenal diseases. Gangliosides play a critical role in bacterial adhesion to cell surfaces. The present study examined how residues of gangliosides are important for inhibition of adhesion of H. pylori to MKN-45 cells. We measured adhesion or detachment effects of gangliosides on the interaction between MKN-45 cells and H. pylori, as well as interleukin-8 production. Among the gangliosides, O-Ac-GD3, GT1b, GD1a, GD1b, GT1a, and GD3 had potent dose dependent inhibitory effects on adhesion of H. pylori to MKN-45 cells, interleukin-8 production, and vacuole formation induced by H. pylori toxin binding to Vero cells. GD3 also accelerated bacterial detachment of MKN-45 cells with adherent H. pylori in a dose dependent manner. Such results strongly suggest that the mechanism involved in the inhibition of H. pylori adhesion is mediated by the variations of the residues of the NeuAc-NeuAc-Gal-Glc chain of gangliosides. NeuAc-NeuAc-Gal-Glc exhibits a more inhibitory effect on adhesion than the NeuAc-Gal-Glc chain. Such gangioside and oligosaccrharide sequences appear to have therapeutic importance for prevention of H. pylori adhesion, as well as reduction of both inflammation and gastric mucosal injuries.
7

Glycoconjugate histochemistry and nNOS immunolocalization in the mantle and foot epithelia of Tapes philippinarum [bivalve mollusc]

63%
Calabro C., Albanese M. P., Martella S., Licata P., Lauriano E. R., Bertuccio C., Licata A.
Folia Histochemica et Cytobiologica
|
2005
|
tom 43
|
nr 3
8

Lysosomal exoglycosidases in serum and urine of patients with pancreatic adenocarcinoma

51%
Szajda S. D., Waszkiewicz N., Stypulkowska A., Dadan J., Zwierz K.
Folia Histochemica et Cytobiologica
|
2010
|
tom 48
|
nr 3
9
Content available remote

New insight into oesophageal injury and protection in physiologically relevant animal models

51%
Zayachkivska O., Pshyk-Titko I., Hrycevych N., Savytska M.
Journal of Physiology and Pharmacology
|
2014
|
tom 65
|
nr 2
10
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The role of complement activity in the sensitivity of Salmonella O48 strains with sialic acid-containing lipopolysaccharides to the bactericidal action of normal bovine serum

51%
Bugla-Ploskonska G., Futoma-Koloch B., Rybka J., Gamian A., Doroszkiewicz W.
Polish Journal of Veterinary Sciences
|
2010
|
tom 13
|
nr 1
53-62
Sialic acids are important constituents of animal tissue glycoconjugates and are also present in the antigens of some bacterial strains. Capsular polysaccharides with sialic acid (NeuAc) have been extensively studied with regard to sensitivity to the bactericidal action of serum, whereas little is known in this regard about lipopolysaccharides (LPS) which contain NeuAc. Strains of Salmonella 048, able to infect animals and containing the same structures of LPS with NeuAc, were examined for their susceptibility to the bactericidal action of normal bovine serum (NBS). The strains showed varied sensitivity to the bactericidal action of NBS, which indicates that the expression of LPS containing NeuAc residues is not critical for the strains' resistance to the serum's activity. In this study the mechanisms of complement activation responsible for killing serum-sensitive Salmonella 048 rods by NBS were also established. Three such mechanisms were distinguished: activation of the classi- cal/lectin pathways, important (decisive) in the bactericidal mechanism of complement activation, parallel activation of the classical/lectin and alternative pathways, and independent activation of the classical and lectin or the alternative pathway.
11

Alcohol abuse and glycoconjugate metabolism

51%
Waszkiewicz N., Szajda S. D., Zalewska A., Szulc A., Kepka A., Minarowska A., Wojewodzka-Zelezniakowicz M., Konarzewska B., Chojnowska S., Ladny J. R., Zwierz K.
Folia Histochemica et Cytobiologica
|
2012
|
tom 50
|
nr 1
12

Alterations in glycoconjugates related to the transformation grade of human uroepithelial cell lines

51%
Ugorski M., Dus D.
Archivum Immunologiae et Therapiae Experimentalis
|
1995
|
tom 43
|
nr 3-4
13

The activity of selected glycosidases in salivary gland tumors

45%
Bierc M., Minarowski L., Wozniak L., Chojnowska S., Knas M., Szajda S., Zwierz K.
Folia Histochemica et Cytobiologica
|
2010
|
tom 48
|
nr 3
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