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The human telomerase catalytic subunit and viral telomerase RNA reconstitute a functional telomerase complex in a cell-free system, but not in human cells

100%

Trapp-Fragnet L., Marie-Egyptienne D. T., Fakhoury J., Rasschaert D., Autexier C.

Cellular and Molecular Biology Letters

2012

tom 17

nr 4

The minimal vertebrate telomerase enzyme is composed of a protein component (telomerase reverse transcriptase, TERT) and an RNA component (telomerase RNA, TR). Expression of these two subunits is sufficient to reconstitute telomerase activity in vitro, while the formation of a holoenzyme comprising telomerase-associated proteins is necessary for proper telomere length maintenance. Previous reports demonstrated the high processivity of the human telomerase complex and the interspecies compatibility of human TERT (hTERT). In this study, we tested the function of the only known viral telomerase RNA subunit (vTR) in association with human telomerase, both in a cell-free system and in human cells. When vTR is assembled with hTERT in a cell-free environment, it is able to interact with hTERT and to reconstitute telomerase activity. However, in human cells, vTR does not reconstitute telomerase activity and could not be detected in the human telomerase complex, suggesting that vTR is not able to interact properly with the proteins constituting the human telomerase holoenzyme.

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Effects of nitroglycerin on energy metabolism of rat reticulocytes

100%

Maletic S. D., Kostic M. M.

Journal of Physiology and Pharmacology

1999

tom 50

nr 1

Acclimation of poplar trees to heavy metals in polluted habitats: I. Carbohydrate metabolism in fine roots of Populus deltoides

75%

Lorenc-Plucinska G., Stobrawa K.

Acta Societatis Botanicorum Poloniae

2005

tom 74

nr 1

Concentrations of total nonstructural carbohydrates (TNC), soluble carbohydrates, starch, sucrose, glucose, fructose, raffinose, galactose, stachyose, mannitol and specific activities of soluble acid (AI) and neutral (NI) invertases, sucrose synthase (SuSy), hexokinase (HK), fructokinase (FK), glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and glucose 6-phosphate dehydrogenase (G6PDH) were analyzed in fine roots of Populus deltoides Bartr. ex Marsh growing at a polluted site (near copper smelters) and a control site (free from heavy pollution). Also chemical properties of the soil from both sites were assessed. In comparison with the control, fine roots from the polluted site contained greater concentrations of TNC, soluble sugars, starch and sucrose but less hexoses, so they had higher values of sucrolysis index (sucrose/hexoses). The activity of AI, NI and SuSy declined insignificantly, while specific activities of HK, FK, GAPDH and G6PDH were significantly inhibited. The results suggest that a long-term heavy metal stress leads to an accumulation of carbohydrates and altering activities of glycolysis and the oxidative pentose phosphate pathway in fine roots.

Ograniczanie wyników

1 Acta Societatis Botanicorum Poloniae

1 Cellular and Molecular Biology Letters

1 Journal of Physiology and Pharmacology

1 Autexier C.

1 Fakhoury J.

1 Kostic M. M.

1 Lorenc-Plucinska G.

1 Maletic S. D.

1 Marie-Egyptienne D. T.

1 Rasschaert D.

1 Stobrawa K.

1 Trapp-Fragnet L.

1 2012

1 2005

1 1999

Wyniki wyszukiwania

The human telomerase catalytic subunit and viral telomerase RNA reconstitute a functional telomerase complex in a cell-free system, but not in human cells

Effects of nitroglycerin on energy metabolism of rat reticulocytes

Acclimation of poplar trees to heavy metals in polluted habitats: I. Carbohydrate metabolism in fine roots of Populus deltoides