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Micromorphological features of glumes, lemmas and paleas were examined under light and scanning electron microscopes in amphiploids of Aegilops geniculata, Ae. peregrina × Secale cereale and their parental species. The principal features include the size and shape of silica bodies, crown cells, prickles, macrohairs, stomata and the morphology of long cells. The SEM observations of glumes separate two parental forms of Aegilops by the type of prickles and types of wax on palea surface. The abaxial surfaces of lemmas in the parental species and amphiploids show the most taxonomical features. Amphiploids in many features exhibit an extended range of variation in comparison to their parental species, particularly in stomatal length. Epidermal features in amphiploids are inherited after Ae. geniculata and Ae. peregrina. Results of this study provided data on new quantitative and qualitative traits of the glumes, lemmas and paleas in the studied taxa.
A pot experiment was carried out on cv. Jasna spring wheat grown on light loamy sand soil. The soil was slightly acidic in reaction and moderately abundant in available phosphorus, potassium and magnesium. Consistent NKMg fertilization rates (1.5 g N, 1.5 g K and 0.25 g Mg) were accompanied by increasingly high doses of phosphorus (0.0 to 1.02 g P per pot). In order to compare the effect produced by growth regulators, applied in conjunction with the growing phosphorus doses, the pots were split into four groups, depending on the sprays applied: distilled water (control), kinetin, gibberellin and auxin. The target was to determine the influence of plant hormones and phosphorus fertilization on nitrogen balance in spring wheat. The content of nitrogen in wheat grain depended mainly on the phosphorus fertilization level, the relationship which became particularly evident following auxin and kinetin application. The highest N concentration occurred when 0.85 g P per pot was used. The growth regulators, auxin and gibberellin in particular, depressed the concentration of nitrogen in grain. The level of nitrogen in glumes and stems was only slightly dependent on the level of phosphorus nutrition. In leaves, it was negatively correlated with the doses of phosphorus. The phytohormones depressed the concentration of nitrogen in stems, oldest leaves and in the flag leaf, raising it in the penultimate leaf. The uptake and accumulation of nitrogen in grain tended to increase up to the phosphorus rate of 0.68 g P per pot. The growth regulators inhibited the accumulation of nitrogen in aerial organs of wheat, especially in grain. Gibberellin, in turn, increased the accumulation of nitrogen in glumes and stems. The contribution of grain in nitrogen accumulation ranged from 62% (without P fertilization) to 68% when 0.68 g P per pot was applied. The growth regulators, especially gibberellin, decreased the ratio of nitrogen accumulated in grain. Protein yield per plant, except wheat sprayed with gibberellin, increased proportionately to the rate of phosphorus.
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