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The aim of this study was to assess the variation in levels of glucose and selected peptide hormones (insulin, leptin and ghrelin) in blood serum of young adults, depending on their nutritional status. Investigations were conducted with the participation of 18 persons, which were divided into three groups characterised by different nutritional status – BMI: <20 kg/m2, 20–25 kg/m2 and >25 kg/m2. Blood samples were collected from each examined person before they have eaten and next blood serum levels were determined for glucose, insulin, leptin, active and total ghrelin. Significant (p<0.05) inter-group differences were shown in leptin concentration, with the highest values of this parameter shown in the group of overweight persons (31.68±23.29 ng/cm3), while the lowest for individuals with BMI<20 kg/m2 (5.81±4.57 ng/cm3). Statistically significant correlations were found between BMI and leptin level (r=0.56, p<0.05), the share of fat mass and levels of insulin (r=0.52, p<0.05) and leptin (r=0.81, p<0.001), as well as the mean skinfolds thickness and the concentration of insulin (r=0.47, p<0.05) and leptin (r=0.63, p<0.01).
Blood samples were taken from 134 Holstein cows between day 20 to 100 postpartum to determine the concentration of urea, cholesterol, glucose and total lipids and to define their relation to the reproductive performance traits: interval (I), service period (SP) and insemination index (II). Investigations were carried out in monthly intervals from October 1996 to October 1997. Average values of plasma urea, total lipids, cholesterol and glucose for the entire period studied were 5.78 mmol.1⁻¹, 3.61 g.l⁻¹, 3.34 mmol. l⁻¹ and 3.61 mmol. l⁻¹, respectively. High concentration of blood urea was related negatively with the value of the insemination index (rp = -0.51). When the average concentrations of total lipids were low in plasma in the specified time (month) negative correlations were found between total lipids and the length of service period (rp = from -0.23 to -0.54) and value of the insemination index (rp = -0.42). The concentration of plasma total lipids increased in time from post partum. Traits of reproductive performance were affected by the internal environment. Especially negative influences were observed when the concentration of plasma urea as well as total lipids and cholesterol increased or decreased abnormally.
Nowak J.: Insulin and glucose concentration changes in newborn piglets after sucking the colostrum from insulin administered sows. Acta Physiol. Pol. Immuno reactive insulin (IRI) concentration in sows colostrum has been previously proven to be much higher than that in blood. The experiment was carried out to show the influence of endogenous and added insulin in sows colostrum on insulinaemia and glycaemia of newborn piglets. In colostrum collected from 3 control and 5 experimental sows before loading, basal insulin concentration were 1.595 and 1.365 nM⁻¹ , respectively, and calculated for all 8 sows together were 1.451 nM⁻¹ (SEM ± 0.289). Basal plasma insulin concentrations calculated for 68 healthy piglets before sucking were little differentiated (mean 0.318 ± 0.044 nM I⁻¹ ), whereas glucose initial concentrations for those piglets (mean 3.581 ± 0.275 nM I⁻¹ ) were highly differentiated. Intramuscular loading of 5 experimental sows with insulin (80 I.U. per animal) caused an increase in the concentration of insulin in colostrum from 1.365 to 3.449 nM I⁻¹ (0.01, P < 0.02). The mean insulin level (0.313 + 0.04 nM I⁻¹ ) in experimental piglets blood plasma (n = 42) increased significantly to 1.234 + 0.07 (P < 0.001) after suckling by sows loaded with exogenous insulin. Glycaemic response of those two piglets litters was poor but showed a statistically significant increase (P < 0.001). The glucose concentrations in blood plasma samples of the other three litters did not changes after sucking. The experiment excluded the hypothesis that high level of insulin in colostrum could be the cause of hypoglycaemia in healthy piglets after sucking.
In diabetes several aspects of immunity are altered, including the immunomodulatory action of adenosine. Our study was undertaken to investigate the effect of different glucose and insulin concentrations on activities of adenosine metabolizing enzymes in human B lymphocytes line SKW 6.4. The activity of adenosine deaminase in the cytosolic fraction was very low and was not affected by different glucose concentration, but in the membrane fraction of cells cultured with 25 mM glucose it was decreased by about 35% comparing to the activity in cells maintained in 5 mM glucose, irrespective of insulin concentration. The activities of 5'-nucleotidase (5'-NT) and ecto-5'-NT in SKW 6.4 cells depended on insulin concentration, but not on glucose. Cells cultured with 10-8 M insulin displayed an about 60% lower activity of cytosolic 5'-NT comparing to cells maintained at 10-11 M insulin. The activity of ecto-5'-NT was decreased by about 70% in cells cultured with 10-8 M insulin comparing to cells grown in 10-11 M insulin. Neither insulin nor glucose had an effect on adenosine kinase (AK) activity in SKW 6.4 cells or in human B cells isolated from peripheral blood. The extracellular level of adenosine and inosine during accelerated catabolism of cellular ATP depended on glucose, but not on insulin concentration. Concluding, our study demonstrates that glucose and insulin differentially affect the activities of adenosine metabolizing enzymes in human B lymphocytes, but changes in those activities do not correlate with the adenosine level in cell media during accelerated ATP catabolism, implying that nucleoside transport is the primary factor determining the extracellular level of adenosine.
One of the variables oscillating seasonally in Helix pomatia L., described in our previous paper, was their rudimentary cryoprotection provided by modest increases in haemolymph glucose and glycerol concentrations in autumn and early spring, respectively. Because factors governing cryoprotective responses are unknown, we decided to investigate the effects of spring and autumn acclimation of H. pomatia to different thermal and photoperiod conditions on the changes in haemolymph concentrations of glucose and glycerol and on the glycogen level in selected organs. Neither acclimation to short-day photoperiod nor low ambient temperature evoked increase in glucose and glycerol concentration in spring and autumn. Both acclimation variants decreased hepatopancreatic glycogen level. The rudimentary freeze-tolerance in H. pomatia seems to be a combined effect of cold and short-day photoperiod and might also be affected by their nutritional and reproductive status. The effect of exposure to frost-bite is also likely to be involved.
The aim of the study was to assess the effect of nutritional status of the organism on the sensory specific satiety (SSS) in young adults and to determine the relationship of blood glucose concentration and SSS. The study was carried out on a set of 18 individuals (6 persons in each group with BMI values: <20, 20-25 and >25 kg/m2, respectively), which rated the pleasantness of foodstuffs with different sensory properties (milk chocolate, crackers, grapefruit and apples) before and after ad libitum consumption of milk chocolate. The SSS was observed in all the tested individuals, irrespective of their BMI value. No statistically significant correlation was found between the change in blood glucose concentration 2 minutes after the consumption of chocolate and the occurrence of SSS. A dependence between these parameters was found 60 min after chocolate consumption.
The present study was designed to evaluate the oxidative stress-related parameters in alloxan-induced diabetes in rabbits. After 3, 6, 12 and 24 weeks of hyperglycaemia the enzymatic and non-enzymatic factors were measured in heart tissue of diabetic and control groups. Superoxide dismutase and glutathione peroxidase activities and the contents of total sulfhydryl compounds significantly increased at all time inter­vals. Catalase activity increased initially (after 3 and 6 weeks), decreased after 12 weeks and increased again at the 24th week of the experiment. Glutathione reductase activity increased initially (at 3rd week), decreased below control level after 6 and 12 weeks, then increased again. Ascorbic acid concentration decreased after 3 and 6 weeks, and increased at the 12th and 24th weeks. The level of lipid peroxidation prod­ucts was reduced after 3,6 and 12 weeks of the experiment. After 24 weeks it was sig­nificantly elevated. These data suggest that hyperglycaemia induces oxidative stress in the heart but the defense mechanisms in the heart tissue are fairly efficacious against oxidative injury.
The present study involved 180 slaughter turkey-hens of heavy Big-6 type divided into four groups (in triplicate repetition for 15 birds). All the birds were fed with the same standard full-dose mixtures in 5-stage system. The turkey-hens of groups I, II and III were given 1,2,4-triasole derivative (3-(2-pyridil)-4-phenyl-l,2,4-triasole-5-carboxylic acid), which has antibacterial, antifungal and immunomodulating properties, in amount of 50, 75 and 100 |ig per 1 dm3 of water. Group IV - control was given water without the additive. The 1,2,4-triasole derivative was given to drinking water, starting from the first day of bird's life and for the whole rearing period. The present results of biochemical analysis of blood plasma showed that addition of examined substance significantly reduced concentration of protein, glucose, triglycerides and uric acid as compared to control. It was stated that tested 1,2,4-triasole derivative elevated the level of HDL fraction percentage and alkaline phosphatase activity in blood plasma.
Dobrze zbilansowana dieta jest jednym z wyznaczników zdrowia. Nieodpowiednie spożycie składników odżywczych może sprzyjać rozwojowi chorób. Celem badania była ocena spożycia wapnia, fosforu, białka i relacja pomiędzy spożyciem wapnia a stężeniem lipidów, glukozy u pacjentów z otyłością. Badaniami objęto 57 osób w wieku 21-63 lat, u których dokonano oceny spożycia na podstawie 3 dniowego zapisu i oznaczono stężenie glukozy, wapnia i lipidów. Stężenie lipidów oceniono metodami enzymatycznymi, stężenie wapnia oceniono aparatem Vitros 250. Średnie dobowe spożycie wapnia u mężczyzn wyniosło 588,8 mg, u kobiet - 549,3 mg i było poniżej poziomu bezpiecznego. Wykazano istotne statystycznie korelacje pomiędzy spożyciem wapnia a stężeniem glukozy u kobiet i mężczyzn. Nie wykazano zależności ze stężeniem lipidów. Spożycie wapnia może być jednym z wyznaczników stężenia glukozy u otyłych osób.
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