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The aim of the studies was to determine the effect of sawdust substrates and their enrichment with glucose on the mycelium growth and yield of three Hericium erinaceus (Bull. Fr.) Pers. strains. The subject of the studies was strains of H. erinaceus designated as ‘H1’, ‘D5’ and ‘D9’. Pine and beech sawdust supplemented with glucose in the amount of 1%, 2% and 3% were used as cultivated substrates. It was found that the tested strains differed in mycelium growth rate. The ‘D9’ strain was characterized by the fastest mycelium growth. The mycelium grew more rapidly on substrate with glucose addition, regardless from its amount, in comparison with substrate without glucose addition. The ‘H1’ and ‘D5’ strains gave the bigger yield than ‘D9’ one. The biggest yield was recorded on beech sawdust substrate with 3% addition of glucose.
Healthy, and insulin-deficient (streptozotocin-induced diabetic, STZ) Sprague-Dawley rats were used to investigate the effects of different doses of ß-endorphin (25 and 50 µg/kg) on plasma ß-endorphin, insulin, glucagon, and glucose levels at 15- and 30-min time points. In experimental groups, plasma ß-endorphin levels were higher at the 15-min than at the 30-min time point in healthy rats; however, in STZ-diabetic rats, ß-endorphin levels were lower at 15 min than at 30 min, indicating that intraperitoneal absorption of ß-endorphin differed between healthy and insulin-deficient rats, ß-endorphin did not affect plasma glucose, insulin, or glucagon at either dose in the healthy group. In the insulin-deficient rats, ß-endorphin at 50 µg/kg reduced plasma glucose levels at the 30-min time point compared to the 25 µg/kg dose, without affecting plasma insulin. Moreover, ß-endorphin at 50 µg/kg decreased plasma glucagon levels at the 15-min time point in comparison to the 25 µg/kg dose in insulin-deficient rats. Plasma glucose levels may be reduced in insulin-deficient rats at high ß-endorphin levels regardless of insulin status.
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Leptin does not seem to influence glucose uptake by bovine mammary explants

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Leptin, a protein produced and secreted by adipocytes, is know to regulate food intake and whole-body energy metabolism, but knowledge about its possible effect in bovine mammary gland is scarce. Leptin may be involved in the regulation of glucose transport even though this effect at the tissue level remains controversial. Once uptaken by the mammary gland, glucose is utilised in several ways but the majority, about 60-70%, is drained for lactose synthesis. This study was aimed at investigating the effect of leptin on glucose regulation in bovine mammary gland. We have examined the effects of leptin on the expression of GLUT1 mRNA, pyruvate kinase (PK) as well as glucose-6-phosphate dehydrogenase (G6PDH) activity. Treatment of mammary gland explants with recombinant leptin did not influence glucose assimilation, pathway transport (GLUT1 mRNA) and glucose metabolism (PK and G6PDH) in this tissue. The results from this study seem to exclude an involvement of leptin in glucose uptake and metabolism in bovine mammary gland.
The aims of this study were to evaluate the influence of an extremely low-frequency electromagnetic field (ELF-EMF) on [³H]glucose uptake in the peripheral tissues and organs of rats. Rats were exposed to EL F-EM F (frequency-10 Hz, induction -1.8-3.8 mT) one hour daily for 14 consecutive days. Control animals were sham exposed. On the 15th day (24 hours after last exposure) rats were injected with D-[³H]-6-glucose 500μCi/kg IP. Fifteen minutes later animals were sacrificed by decapitation and peripheral tissues were excised and examined for radioactivity (desintegrations per minute, DPM/100 mg wet tissue weight), which expressed [³H]glucose uptake. In most of the examined tissues and organs, such as liver, kidney, heart muscle, cartilage, connective tissue, tendon and skin, [³H]glucose uptake in EL F-EM F-exposed animals was significantly higher as compared to that in the sham control. Exposure to EL F-EM F did not influence [³H]glucose uptake in the thoracic aorta and the skeletal muscle. It is concluded that ELF-EMF impacts tissue glucose uptake by facilitating glucose transport via cell membranes, dependent and probably also independent of its role in increasing insulin action in insulin-dependent tissues.
Structural parameters of retrograded amylose chains in aqueous solution were determined by means of a comparative analysis with glucose solutions stored under the same conditions and the same specific volume. Viscosimetric and Polarimetrie measurements were carried out in glucose and amylose (from potato) solutions during storage, within the same concentration range of 0.005-0.02 (g/cm3), and with a fixed low concentration of a polymer complexing agent (5 10 -5 g/cm3), added as a polymer chain stiffener. From the ratio of limit viscosity numbers obtained for polysaccharide and monosaccharide solutions, the average asymmetric parameter of rigid amylose chain was calculated, value of which points to a significant length expansion of retrograded α-D-glucopyranose coil. Measurements of the concentration dependence of the optical rotatory dispersion in retrograded amylose and its monomer unit solutions were used to determine the degree of coiling into helix of polysaccharide in aqueous solution.
Concentration of free cytoplasmic Ca2+ ([Ca2+]i) in Ehrlich ascites tumour cells loaded with fura-2 was measured in single cells applying a video imaging system. In resting cells [Ca2+li amounted to 60 - 340 nM and was increased after addition of 10 mM D-glucose or D-2-deoxyglucose by 80 - 200 nM. This increase occurred within 30 - 60 s following addition of the sugars and lasted for several minutes. Pretreatment of the cells with thapsigargin resulted in a much smaller [Ca2+]i increase after addition of glucose or deoxyglucose and, vice versa, thapsigargin added after the sugars mobilized less Ca2+ than when added before. A possible relation of the [Ca2+]i rise evoked by glucose and deoxyglucose to the Crabtree effect is discussed.
W pracy przeprowadzono analizę wpływu czynników technologicznych warunkujących uzyskiwany skład granulometryczny glukozy krystalicznej w procesie jej krystalizacji. Badania zrealizowano na zagęszczonym syropie glukozowym (uzyskanym na bazie enzymatycznego rozkładu skrobi pszennej) pobranym z linii produkcyjnej Przedsiębiorstwa Przemysłu Spożywczego PEPEES S.A. Łomża. Jako podstawowe parametry determinujące przebieg procesu krystalizacji wybrano: ilość szczepu krystalicznego i szybkość chłodzenia. Analizę składu granulometrycznego glukozy prowadzono w oparciu o obrazy kryształów uzyskiwane w warunkach interferometrii birefrakcyjnej. Pomiary dokonywano z zastosowaniem programu do komputerowej analizy obrazów analySIS. Następnie używając programu STATISTCA 8.0 charakteryzowano rozkłady populacji kryształów uzyskiwane w próbkach. Wszystkie badane partie glukozy krystalicznej charakteryzowały się populacjami wykazującymi charakter rozkładu wykładniczego. Wykazano, że w wyniku użycia szczepu krystalicznego na poziomie 6% masy krystalizującej mieszaniny uzyskuje się skład granulometryczny cechujący się najniższą wartością parametru rozkładu wykładniczego λ = 0,019 μm-1. oraz najwyższą średnią wielkością kryształów d50 = 52,46 μm. Stwierdzono również, że populacje kryształów uzyskiwane przy wolnym chłodzeniu istotnie różnią się parametrem λ i medianą kryształów d50 w odniesieniu do próbek krystalizujących przy szybkim chłodzeniu. Uzyskane wyniki pozwalają jednoznacznie stwierdzić, że prowadząc krystalizację w kontrolowanych warunkach istnieje możliwość znaczącej korekty składu granulometrycznego glukozy krystalicznej.
Hyperglycemia-induced alterations of adenosine receptors (ARs) expression are implicated in the pathomechanism leading to impaired function of the lymphocytes in diabetes. However, the signaling pathways utilized by glucose to regulate ARs expression are unknown. This work was undertaken to investigate the impact of high glucose level on the ARs expression in rat B lymphocytes. The results presented in this report demonstrate that rat B lymphocytes express all four types of ARs at the mRNA and protein level. Exposing B cells to high glucose (25 mM) suppressed the expression of A1-AR, A2B-AR, and A3-AR, but had no effect on the expression of A2A-AR. A selective inhibitor of Ca2+-dependent protein kinase C (PKC) isoforms suppressed the high glucose effect on A3-AR expression. Inhibition of PKC- with rottlerin blocked the high glucose effect on A1-AR mRNA level. An inhibitor of Raf-1 kinase completely blocked the high glucose effect on A2B-AR expression. The suppression of A1-AR and A2B-AR mRNA expression induced by high glucose was blocked by an inhibitor (PD98059) of MAPK kinase (MEK). In conclusion, high glucose utilizes a signaling pathway involving some elements of the MAPK pathway and different PKC isoforms to suppress the expression of A1-AR, A2B-AR, and A3-AR in rat B lymphocytes.
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