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The objective of this study was to determine the effect of long-term (48 d), per os animal administration of low zearalenone (ZEA) doses (50% and 100% NOAEL values) on the dynamics of changes in the morphometric parameters of the reproductive organs in sexually-immature gilts. The experiment involved 12 clinically-healthy gilts aged 2 months with initial body weight of ± 40 kg and a determined immune status. The animals were randomly divided into two experimental groups (El, n=4; E2, n=4) and a control group (C, n=4). Group El was administered per os 20 µg of ZEA/kg b.w. for 48 d, group E2 received per os 40 µg of ZEA/kg b.w. for 48 d, and group C was administered per os placebo for 48 d. The mycotoxin was administered daily per os animal in gelatin capsules before morning feeding. The animals were slaughtered at the end of the experiment. No significant morphometric changes were noted in the reproductive system of the gilts, except for an increase in the number of medium-sized ovarian follicles in group El. This suggests that ZEA at low concentrations may cause hormonal effects (hyperoestrogenism) but it does not exhibit xenobiotic activity.
The purpose of this study was to determine whether inflammatory changes in uterus caused by Escherichia coli are associated with changes in nitric oxide (NO) production. Therefore, the activity of NADPH-diaphorase (NADPH-d), a histochemical marker for nitric oxide synthase (NOS), localization of inducible isoform of NOS (iNOS) and tissue content of nitrite were studied in the uterine structures after inoculation of Escherichia coli into the uterus in gilts. Twelve sexually matured gilts with controlled estrous cycle were used. The animals were laparotomized on the 2nd day of the estrous cycle and polyvinyl cannulas were inserted into the uterine horns to infuse saline or Escherichia coli. In the group I (control; n=6), 25 ml of saline was infused into each uterine horn on the 4,h day of the estrous cycle. At the same time, 25 ml of Escherichia coli (strain 025:K23/α/:Hl) suspension, containing 107 colony forming units/ml was inoculated into each uterine horn of the treated gilts (group II; n=6). The middle part of the uterine horn was collected on the 14th day of the next estrous cycle immediately after slaughter. Cryostat sections from paraformaldehyde fixed tissues were stained histochemically to study the activity of NADPH-d and immunohistochemi- cally to investigate the distribution of iNOS. Optical density was assessed to evaluate the intensity of the histochemical reaction. Nitrite content was measured spectrophotometrically. In the Escherichia coli-treated gilts, the activitý of NADPH-d in the luminal epithelium and in external parts of excretory ducts of uterine glands was higher (P < 0.001) as compared to that in the control animals. In the secretory part of the uterine glands the activity of this enzyme was similar in both groups of the gilts. In the gilts that received Escherichia coli, the histochemical reaction of NADPH-d in endometrial blood vessels was stronger than that found in the control animals. Immunoreactivity for iNOS in the luminal epithelium, in external parts of excretory ducts of uterine glands and in vascular endothelial cells was stronger in the Escherichia coli-treated gilts as compared to that observed in the control animals. Only weak or no immunoreactivity was found in the secretory part of the uterine glands in both groups of the gilts. After Escherichia coli inoculation, nitrite content in the uterine tissues was higher (P < 0.05) than that determined in the controls. Our study has revealed that infusion of Escherichia coli into the porcine uterus induces the activity of NADPH-d, iNOS and increases the tissue content of nitrite in this organ. The data obtained indicate that NO can mediate an inflammatory effect of Escherichia coli in the uterus.
In gilts, the period of early pregnancy occurring from the time of fertilization to the beginning of implantation is sensitive to any environmental disruptions, including an unbalanced diet of a future mother. Previously, we found that due to the undernutrition in gilts during this period, the endocrine intrauterine microenvironment and DNA methylation in the uterus have been changed. These distortions may diminish the success of pregnancy. In this study we focused on the influence of a restricted diet used in gilts during the first days of pregnancy on their biochemical and haematological parameters in peripheral blood. The applied restrictive diet vs. normal diet covered only 70% of the nutritional demands of early pregnant gilts. Normal (n = 4 gilts) or restrictive (n = 5 gilts) diets were used from the day of the first signs of the estrus until day 9 of pregnancy and biochemical and haematological parameters in blood plasma were determined during peri-implantation period, e.g. on days 15 to16 of pregnancy. In restrictive vs. normal fed gilts significantly lower plasma phosphorus, calcium and total cholesterol as well as the tendency to increasing concentrations of triglicerydes and asparate aminotranserase were found. Haematological parameters did not differ between the studied gilts. Thus, it seems that the availability of nutritional factors became suboptimal in restrictively fed early pregnant gilts. Even short-lasting undernutrition of females during the peri-conceptional period may cause a disruption of biochemical homeostasis during the peri-implantation period and probably affect the success of pregnancy.
The aim of the study was to evaluate the influence of low dose (LOEL - lowest observed effect level) of zearalenone (200 μg/kg b.w.), applied per os for 7 days (short-term intoxication), on sexual behavior, concentration of the examined xenobiotic and its metabolite and selected estrogens in sexually immature gilts: ovariohysterectomised (group Dl) and intact (group D2) animals. Clinical signs of oestrus (reddening, oedema and hyperaemia of the vulva and serorhoea from the reproductive tract - lack of standing reflex) were obserwed in group Dl on day 6 and in group D2 on day 4 of the experiment. Laboratory analyses of blood plasma were carried out determine the presence of zearalenone and a-zearalenole. They revealed an increase in the level of a-zearalenol before the oestrus, decrease in total amount of both examined substances on day when the oestrus appeared and increase in the level of both examined xenobiotics in the post oestrus period together with the higher share of zearalenone. Medium concentrations of estrone and estradiol within the borders of method determination in the majority of periods examined. Higher levels of estrone (32.0 pg/ml) were found on day 4, in the group D2 and estradiol (6.5 pg/ml) on day 6 in the D2 group. The presents study revealed that zearalenone applied per os at LOEL dose causes the incidence of apparent sexual readiness (without standing reflex) in sexually immature gilts with the somatically immature reproductive system.
This study was designed to establish: a) whether boar pheromones, androstenone and androstenol, may affect the vasocontractility of the facial superficial veins in ovariectomized pubertal gilts and b) what is the effect of estradiol on this contractility. The gilts ovariectomized after two controlled estrous cycles, and the ovariectomized gilts treated with estradiol benzoate were used in the experiment. The isolated rings of dorsal nasal, frontal and facial veins were incubated with androstenone (5a-androst-16-en-3-one) and androstenol (5a-androst-16-en-3-ol) in concentrations of either 1 or 10 μM. Changes in the contractile activity of the isolated vein segments were measured using isometric transducer and recorded with HSE-ACAD W software. In ovariectomized gilts both the androstenone and androstenol caused a relaxing effect on the nasal vein, flow of the blood from the nasal cavity, and on the frontal vein, by which the blood may by directed into the perihypophyseal vascular complex. An opposing reaction to these pheromones was found in the distal part of the facial vein by which the blood is directed to the systemic circulation. Treating ovariectomized gilts with estradiol benzoate changed mainly the reactivity of the frontal vein to androstenone, which produces constriction, but this treatment did not affect the reactivity of the facial superficial veins to androstenol. The present results demonstrated that both boar sex pheromones, androstenone and androstenol, may contribute to the regulation of their humoral pathway from the nasal cavity to the brain and hypophysis in the ovariectomized pubertal gilts and suggest the effect of estradiol to this pathway.
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