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Anisoptera scaphula (Roxb.) Kurz, the tallest tree species in Bangladesh, is critically endangered worldwide due to its poor natural regeneration. This study describes the appropriate regeneration technique for A. scaphula through seed germination and clonal propagation by stem cutting. The fastest and highest germination percentage (65) was observed in dewinged seeds sown inverted orientation with half buried position (T10) followed by dewinged seeds (62.5%) sown horizontally in full buried (T7) and delayed germination with lowest percentage (15) was in control (T0). Optimum initial growth was also shown when seedlings were developed from the seeds under T7. Both winged and dewinged seeds sown vertically in full buried or half buried position produced curved seedlings. However, dewinged seeds sown in horizontal or inverted (winged side down) position produced otherwise i.e., straight seedlings. The highest rooting percentages (63%) and maximum number of root (4.8) per cutting were obtained when one node cuttings treated with 0.8% IBA solution followed by 0.4% IBA in turn affecting for better survival and growth of rooted cuttings in the nursery conditions.
The physico-chemical parameters of the content in the effluents from Rajasree sugar factory of Villupuram district have been explored and its impact on the germination and growth patterns of black gram varieties has been studied. Physico-chemical parameters included color, odour, pH, temperature, electrical conductivity, suspended solids, dissolved solids, BOD, COD, chloride, sulphate, calcium, oil and grease concentration. Germination studies was conducted with black gram seed varieties (ADT-3, ADT-5, Vamban-3, Vamban-5 and Co-6) treated with different concentrations (control, 5, 10, 25, 50, 75 and 100 % ) of sugar mill effluent. Germination studies parameters such as germination percentage, germination index, shoot length, root length, fresh and dry weight of seedlings were found to be increased up to 10 % concentration of effluent. Vigour index, tolerance index, percentage of phytotoxicity and germination index were also calculated. The above mentioned parameters were decreased with the increase of effluent concentrations (25-100 %).
Head smut of corn is caused by the fungus Sphacelotheca reiliana and occurs in northeast China and in regions of a similar climate. Yield losses due to the disease are variable and directly depend on the severity of the disease. The objective of this study was to produce a coating technology to protect corn from head smut and to avoid environmental pollution. Based on its excellent properties of high efficiency, nonpollution and nontoxicity, a novel seed coating agent was prepared with modified chitosan as the main material and trace elements and fertilizer as the auxiliary material. Compared with the conventional toxic seed coating agent, the novel seed coating agent protected the seeds and provided excellent control of head smut and increased yield by 11.6 to 14.6%, while the cost of seed coating agent decreased by 32.4%. Our findings indicate that the application of chitosan in seed coating technology had a remarkable effect on the resistance to head smut of corn and yield enhancement.
The present study was conducted in order to determine the effect of gamma rays and EMS on seed germination, Seedling height and root length in chick pea to identify the lethal dose (LD50). In this regard, the healthy seeds of chick pea was subjected to different doses/concentrations of gamma rays (20, 30, 40, 50 and 60kR) and EMS (10, 20, 30, 40 and 50mM) for inducing mutation. The effect of gamma rays and EMS was determined by measuring the seed germination, seedling height and root length under the conditions of the M1 generation. The results shows that, the seed germination, seedling height and root length were significantly decreased with increasing doses/concentrations. The LD50 values were observed based on the growth reduction of seedlings after treatments with mutagen. The effective doses/concentrations which caused 50% growth reduction were observed in 40kR in gamma rays and 30mM in EMS.
Effect of plant growth regulators on seed germination and seedling characters in terms of root length and shoot length. The seeds of sesame variety TVM – 1 were treated with different concentration of gibberlic acid (1.0, 2.0, 3.0 and 4.0 mg/l) and indole acetic acid (1.0, 2.0, 3.0 and 4.0 mg/l). From the results, it was observed that the GA3 and IAA at 2.0mg/l had regulatory effect on seed germination and seedling characters. Maximum germination, root length and shoot length was observed at 2.0mg/l in GA3 and 2.0mg/l in IAA than control. And GA3 at 2.0gm/l was found to be more efficient to modify seed germination and seedling characters when compared to IAA and control.
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