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Stachys annua is a short-lived archaeophyte. In Poland it has been classified as threatened with extinction (VU). It prefers carbonate soils and occurs as a weed in cropped and stubble fields in traditionally farmed areas. Study of the occurrence of Stachys annua in North-Eastern Poland was conducted in 2009–2012 at four sites where the species occupied a minimum area of 30 m² and occurred at each stage of a 4-year crop rotation (spring cereals, winter cereals, tuber crops and stubble fields). The floristically richest communities (25–34 species) were established in unploughed stubble fields, regardless of the habitat conditions. Phytocenoses with S. annua included rare species as demonstrated by high values of floristic value index (FV ranges from 25 to 36). Principle Component Analysis and Spearman rank correlations between plant traits and habitat conditions pointed to soil pH as the factor which determined S. annua phenotypic variation, in particular its height and seed production. The greatest phenotypic variation was found on the alkaline habitat, rich in nitrogen. On the other hand, the cluster analysis showed that the studied populations of inert nitrogen-poor habitats were the most similar. Electrophoretic analysis of storage proteins from S. annua seeds revealed a relatively high genetic similarity of studied populations which was reflected in the similarity coefficients — 0.76 and 0.61 by Dice and Jaccard, respectively. In the case of small or fragmented populations this poses a threat associated with inbreeding and genetic drift of S. annua, which indicates that populations of this species in the study area need to be protected from extinction.
Hexaploid triticale has many advantages over both parental species for both grain and forage production in certain environments. Additional information on environmental stability and heritability would be desirable to develop appropriate selection strategies in the production of superior widely-adapted cultivars. The grain yield of 22 diverse genotypes grown at four ecologically-distinct geographical locations [Quincy, FL, USA (approximate geographical coordinates (AGC) = 30°N 84°W, approximate elevation (AE) = 58 m), Plains, GA, USA (AGC = 32°N 84°W, AE = 76 m), Bozeman, MT USA (AGC = 45°N 111°W, AE = 1458 m), and Aberdeen, ID, USA (AGC = 42°N 112°W, AE = 1360 m)] was measured in two years with winter and spring planting dates only at Bozeman and Aberdeen. Test weight (grain weight in a given volume) was determined for two years at Bozeman and Aberdeen at both planting dates and one year at Quincy. Stability analyses indicated that significant (P <0.01) variation in means, regression coefficients, and deviation mean squares of the genotypes were present for both characters. Realized heritability (h²) estimates were as follows: grain yield ranged from -0.02 to 0.80 with a mean of 0.57; test weight ranged from 0.63 to 1.05 with a mean of 0.93. The results indicated that substantial genetic variation is present and selection for widely-adapted cultivars would be effective for both characters especially test weight.
Variation in the level of self-incompatibility (SI) was quantified in 14 Polish lines of cabbage (Brassica oleracea L. var. capitata) pollinated in various temperature/humidity conditions. Two methods were used to measure the expression of incompatibility: counts of seed set and counts of pollen tubes penetrating the styles 48 h after self-pollination. The level of self-incompatibility varied distinctly depending on the cultivar (from 0 to 65% seed-containing siliques). The genetic background of the material determined the influence of environmental factors on SI. Lower temperature (12°C) and higher humidity (90%) positively affected bud pollination, possibly due to a less stressful protocol. The observed great genotypic variation suggests it may be difficult to find a universal method suitable for breaking the self-incompatibility barrier for the species. The method may have to be tailored to the particular genotype.
Genotype-dependent variation in water stress-induced tissue dehydration (RWC), proline accumulation (PA), membrane injury (MI) and stress resistance (R) was studied among old and modern cultivars of winter wheat (Triticum aestivum L.). Twenty-day- old seedlings grown in nutrient solution were subjected to a moderate water stress (-0.75 MPa) by immersing (for 72 h) their root systems in aerated nutrient solution with the addition of polyethylene glycol (PEG 6000). Both cultivar groups tended to exhibit a different responsibility to the moderate stress. Water deficit induced reductions in the water content of leaves in five out of nine modern cultivars only and in these cultivars an increased accumulation of free proline was found. The highest, about 100-fold increase in PA was noticed in the modern cvs Emika and Finezja. The highest MI (≈ 12%) had the modern cv. Finezja. The leaf PA under stress positively correlated with MI, but both characteristics showed negative correlations with RWC. Only weak and insignificant relationships between leaf characteristics and stress resistance were observed. The results suggest that none of the individual measured physiological parameters would indirectly serve as a precise indicator for drought resistance in the studied wheat collection.
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