Wyniki wyszukiwania

Wyszukiwano:
w słowach kluczowych: genomic species

Sortuj według:

Ogranicz wyniki do:

Possibilities in identification of genomic species of Burkholderia cepacia complex by PCR and RFLP

100%

Navratilova L., Chroma M., Hanulik V., Raclavsky V.

Polish Journal of Microbiology

2013

tom 62

nr 4

The strains belonging to Burkholderia cepacia complex are important opportunistic pathogens in immunocompromised patients and cause serious diseases. It is possible to obtain isolates from soil, water, plants and human samples. Taxonomy of this group is difficult. Burkholderia cepacia complex consists of seventeen genomic species and the genetic scheme is based on recA gene. Commonly, first five genomovars occurre in humans, mostly genomovars II and III, subdivision IIIA. Within this study we tested identification of first five genomovars by PCR with following melting analysis and RFLP. The experiments were targeted on eubacterial 16S rDNA and specific gene recA, which allowed identification of all five genomovars. RecA gene appeared as more suitable than 16S rDNA, which enabled direct identification of only genomovars II and V; genomovars I, III and IV were similar within 16S rDNA sequence.

Human pathogenic borreliae in Ixodes ricinus ticks in natural and urban ecosystem (Czech Republic)

100%

Venclikova K., Betasova L., Sikutova S., Jedlickova P., Hubalek Z., Rudolf I.

Acta Parasitologica

2014

tom 59

nr 4

A total of 1279 field-collected Ixodes ricinus ticks were screened for Borrelia burgdorferi sensu lato (s.l.) in a natural and an urban ecosystem of Ostrava city (Czech Republic) by using molecular methods. Minimal prevalence rate for Borrelia burgdorferi s.l. in ticks for the urban park Bělský les was found to be 13.8% (17.6% in males, 17.8% in females and 11.7% in nymphs), similarly for the natural site Proskovice was minimal prevalence 15% (12.5% in males, 20% in females and 14.9% in nymphs). Six proven human pathogenic genomic species have been recorded in the study: B. afzelii, B. garinii, B. burgdorferi s.s., B. valaisiana, B. lusitaniae, and B. spielmanii. Emerging B. spielmanii was detected for the first time in Ixodes ricinus ticks in the region. Our results highlight the need for surveillance of zoonotic tick-borne pathogens even in urban areas.

Ograniczanie wyników

1 Acta Parasitologica

1 Polish Journal of Microbiology

1 Betasova L.

1 Chroma M.

1 Hanulik V.

1 Hubalek Z.

1 Jedlickova P.

1 Navratilova L.

1 Raclavsky V.

1 Rudolf I.

1 Sikutova S.

1 Venclikova K.

1 2014

1 2013

Wyniki wyszukiwania

Possibilities in identification of genomic species of Burkholderia cepacia complex by PCR and RFLP

Human pathogenic borreliae in Ixodes ricinus ticks in natural and urban ecosystem (Czech Republic)