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To map the QTLs of Fusarium moniliforme ear rot resistance in Zea mays L., a total of 230 F₂ individuals, derived from a single cross between inbred maize lines R15 (resistant) and Ye478 (susceptible), were genotyped for genetic map construction using simple sequence repeat (SSR) markers and amplified fragment length polymorphism (AFLP) markers. We used 778 pairs of SSR primers and 63 combinations of AFLP primers to detect the polymorphisms between parents, R15 and Ye478. From the polymorphic 30 AFLP primer combinations and 159 SSR primers, we scored 260 loci in the F₂ population, among which 8 SSR and 13 AFLP loci could not be assigned to any of the linkage groups. An integrated molecular genetic linkage map was constructed by the remaining 151 SSR and 88 AFLP markers, which distributed throughout the 10 linkage groups of maize and spanned the genome of about 3463.5 cM with an average of 14.5 cM between two markers. On 4 chromosomes, we detected 5 putative segregation distortion regions (SDRs), including 2 new ones (SDR₂ and SDR₇). The other 3 SDRs were located near the regions where gametophyte genes were mapped, indicating that segregation distortion could be partially caused by gametophytic factors.
Advanced knowledge of the canine genome facilitates progress in studies on genome maps of other canids, including species considered also as farm animals. In this study canine BAC (Bacterial Artificial Chromosome) probes, harbouring three genes involved in sex determination (SOX9 - sex determining region Y- box 9, AMH - anti-Müllerian hormone and AR - androgen receptor), were mapped in the dog, red fox, arctic fox and Chinese raccoon dog chromosomes. Localization of these genes can be helpful in association studies focused on monogenetic intersexual disorders.
Fat content of carcass is an important multigenic trait in pig breeding. There are reports indicating several chromosomes, e.g. 1, 2, 4, 5, 6, 7, 12, 13, 14 and 18 which possibly harbour QTLs for fatness traits. Among QTL candidate genes there are leptin (LEP) and its receptor (Lepr), both playing essential role in food intake and energy balance. Moreover, expression level and polymorphism of the adipocyte specific transcription factors, such as CREB (cAMP response element binding protein) or C/EBP (CCAAT/enhancer-binding protein) may also cause phenotypic variation of the fatness traits. Some of the candidate QTLs, as Lep, Lepr, C/EBP and additionally H-FABP (fatty acid binding protein gene) and RYR1, are localized on chromosome 6. It is foreseen that searching for polymorphisms of the chosen genes may reveal association between a genotype and phenotypic variation of selected fatness traits. However, the studies are complex and require analysis of numerous genes.Cited are 71 references.
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