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The present study was under taken in order to analyze the chemical mutagenesis on Chilli germplasm. In this regard, K1 variety of chilli was subjected to different mutagenic concentration for inducing mutagenesis. The M3 plants exposed to EMS and DES to produce clear difference from the untreated control, thus indicating that mutagenic treatment produce polymorphic regions in the chilli. For extraction of genomic DNA was adopted an improved protocol of CTAB method with slight modification. A total of ten primers were used to screen the polymorphism among the treated populations line tall, tall with chlorophyll deficient, leaf, flower, GMS and DNA damages in maturity mutants were analyzed with control. Out of ten primers, four primers (PGF02, PGF03, PGF04 AND OP107) were successfully amplified in all the samples used for this study. The successful primers were amplified in to 93 products showing an average of 9.3 bands.
In some families of large mammals (Cervidae, Bovidae, Canidae), we examined relationships between the various mating systems adopted and biochemical-genetic variation measured in terms of the mean proportion of polymorphic loci (P), mean heterozygosity (H) and derived coefficients, such as the ratio Pt:P and FIS. Our hypothesis was that genetic variability decreases as the degree of polygyny of the mating system adopted increases. Most of the data were in accordance with this prediction, but also some ambiguous results could be observed. Methodological and practical difficulties connected with our synoptical approach, such as the lack of comparability among most electrophoretic studies and the general scarcity of quantitative behavioural data are critically discussed.
After duplicating the chromosome number by colchicine in sterile F₁ 16-chromosome hybrid T. pratense × T. diffusum some partially fertile plants with 32 chromosomes were found. Male fertility (viability of pollen grain) was from 69.3% to 86.2% (on average 81.8%), whereas female fertility estimated as seed setting after cross- and self-pollination was 21.8% and 6.9%, respectively. Male and female fertility as well as somatic chromosome number were examined in the F₂-F₄ generations. Selection for female fertility resulted in increasing seed setting in the first two generations (F₂ and F₃) and in decreasing it in F₄ generation. An average seed setting in the F₂-F₄ generations after cross-pollination amounted to 22.2%, 43.6% and 12.9%, respectively; after self-pollination it was 25.2%, 27.6% and 1.9%. In the F₂ generation all the plants had 2n=32 chromosomes. In the next generations there appeared aneuploids, among which 30-chromosome individuals were predominant.
Based on previously published electrophoretic data on genetic variability in 31 roe deer Capreolus capreolus Linnaeus, 1758 populations, the proportion of loci polymorphic (P), average heterozygosity (H), and the inbreeding coefficient (FIS) were examined for relationships with the social structure displayed in the various populations. Our hypothesis was that genetic variability is lower and FIS-values are more positive in populations where males maintain a stable pattern of territories during the rutting season (forest dwelling roe deer) than in those characterized by pronounced fluctuations in population structure, both within and among seasons (field or mountain dwelling roe deer). P and H did not show differences among those two groups. FIS was significantly more positive in the 'forest' roe deer than in the more migratory 'type', but only when populations subjected to high culling rates were excluded from the analysis. Highly negative FIS-values in forest populations with high culling rates suggested that considerable perturbations of population structure may be caused by hunting. In conclusion, the 'forest' roe deer and the 'field' roe deer do not represent two distinct ecotypes with a particular genetic integrity, but rather reflect the considerable behavioural plasticity of the species.
The experiment was performed on 121 Jersey cows in the years 2003/2004. The aim of this study was to determine the yields of ECM, milk fat and milk protein as well as milk fat content and milk protein content during four successive 305-day lactations as dependent on the genotypes and polymorphic variants of milk proteins. Depending on their genotype, the cows were divided into the following groups: AA, AB, BB and BC. Four major milk proteins were obtained as a result of separation, i.e. LGB, CSN1S1, CSN2 and CSN3. The yields of ECM, milk fat and milk protein increased during four successive 305-day lactations. Cows with the polymorphic fractions LGB AA and AB, CSN1S1 BC, CSN2 AB and CSN3 AA were characterised by the highest productivity. The highest increase in fat content (0.63%) was recorded between the first and second lactation. The LGB AA genotype contributed to an increase in protein concentration, by 0.14%, 0.12% and 0.19% in the second, third and fourth lactation, respectively. In the case of the other fractions these values were as follows: CSN1S1 BC - 0.13%, 0.07% and 0.11% in the second, third and fourth lactation, respectively, CSN2 AA - 0.09%, 0.20%, 0.08% and 0.04% in the first, second, third and fourth lactation, respectively, and CSN3 BB - 0.11%, 0.16% and 0.10% in the second, third and fourth lactation, respectively.
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