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In this study the sequences of the long terminal repeat (LTR) of field isolates of the bovine leukaemia virus (BLV) were analysed. These isolates came from emerging cases of BLV infection in cattle from herds having BLV-free status. We found several sequence variations within regulatory motifs in the LTRs like GRE, DAS and interferon binding site. These mutations can possibly affect transcriptional activity of the virus, leading to its silencing.
Breeding endangered mammals for their conservation requires knowledge about the genetic architecture of the respective species. In taxa with tight genetic cohesion between populations, the definition of management units for captive breeding rarely poses problems, except if there are morphologically well differentiated subspecies grading into one another although they are hardly separate at the molecular level. Species with genetic diversity predominantly between populations can pose serious problems for breeders. Examples are discussed of mammalian species with complex genetic architectures, where decisions have to be drawn whether to select only certain populations for conservation, or to create an artificial taxon. Research into subspecific molecular taxonomy of rare zoo-living wildlife is frequently hampered by small sample sizes available for study, with the risk of spurious molecular taxonomie distances based on marker allele frequencies in populations influenced by genetic drift. "Typological" approaches are suggested for molecular systematics of such study material, with the haplotype organization of polymorphic MHC genes appearing particularly promising. Additional molecular approaches, not easily susceptible to sample size problems, are shortly presented. The implementation of breeding plans to achieve conservation genetic goals may interfere with the social structures of the animals. This group of problems includes the transfer of socially compatible individuals to form new groups, and the provision of suitable sexual partners for mate choice mechanisms to act in their species-specific manner. A survey of scientific research in European zoos is provided to recognize what is being done to fight the current ignorance about basic aspects of the conservation biology of many endangered mammals.
Pepino mosaic virus (PepMV) has emerged as an important pathogen of greenhouse tomato crops and is currently distributed worldwide. Population genetic studies have revealed a shift in the dominant PepMV genotype from European (EU) to Chilean 2 (CH2) in North America and several European countries. New genetic variants are constantly being created by mutation and recombination events. Single nucleotide substitutions in different parts of the genome were found to affect on development of symptoms resulting in new pathotypes and accumulation of viral RNA. The variability of the PepMV population has a great impact on designing specific diagnostic tools and developing efficient and durable strategies of disease control. In this paper we review the current knowledge about the PepMV population, the evolutionary dynamics of this highly infective virus, methods for its detection and plant protection strategies.
A sample of Betula nana from the Linie reserve near Dąbrowa Chełmińska, have been fingerprinted using random amplified polymorphic DNA (RAPD). The high level of genetic variation was detected. All individuals had unique genotypes, supporting the generally high resolving power of RAPD’s. For the conservation strategy, information about the distribution of the genetic variation within and among populations plays very important role. Thus, extensive study in other populations of dwarf birch is needed.
Ninety-seven tomato landraces collected from East Anatolian region of Turkey and North-West of Iran, along with three commercial cultivars were evaluated during two years. Experiment was carried out in an alpha lattice design at Agriculture and Natural Resources Research Center of West Azerbaijan, Iran. Analysis of variance revealed significant variation (P ≤ 0.01) among genotypes for all the experimental characters. Yield showed a positive and significant correlation with length and width of cotyledon leaf, length and width of true leaf, fruit weight, fruit length and diameter, pericarp thickness and fruit peduncle length. In principal component analysis, the first three components explained for 71.6% of total variations among genotypes. Since the first component determined 50% of total variations and yield had high significant coefficient with this component, thus it might be used as s selection criteria to identify genotypes with high yield in breeding programs. Cluster analysis using Ward method classified genotypes into five groups. Groups included: early maturing genotypes in group I, genotypes with high yield in group II, genotypes with large fruit in group III, late maturing and high total soluble solids (TSS) genotypes in group IV and genotypes with high acidity in group V.
The greater mouse-tailed bat (Rhinopoma microphyllum) possesses a large geographical range, covering most of the arid and warm areas of the Old World. We studied the genetic variability of this species using two mitochondrial markers (the cytochrome b gene and the control region), from several Israeli colonies and from over most of the species' range. Our results show that the cytochrome b sequences, unlike those of the control region, are too conserved to separate among R. microphyllum populations. Based on the control region sequences, a high level of sequence similarity was found within the Israeli population. Three clades were observed over the species' range: Oriental, Intermediate and Palaearctic. This division supports most of the traditional taxonomy of the species. The Israeli population, which belongs to the Palaearctic clade, was found to be isolated from the Oriental and Intermediate clades. We suggest that the colonization of the greater mouse-tailed bat in the Levant occurred from African populations during the late Pleistocene, when many Saharan plants and animals penetrated the northern part of the Great Rift Valley.
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