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The advantage of the clonal plants over the nonclonal species in colonization of all biomes and biogeographical regions primarily is due to occurrence of two modes of reproduction. The creating of vegetative propagules with high vitality and survivability, enables the spread in the newly colonized site, while seed production increases the chances for successful recruitment of seedlings, ensuring the genetic diversity of the population. The differences in allocation into vegetative reproduction and generative propagation in relation to rising height of adjacent plants was investigated in populations of Serratula tinctoria L., occurring in Molinietum caeruleae meadows localized in Southern Poland. Each locality was represented by randomly established three permanent study plots: dominated by small meadow species (LOW), prevailed by large-tussock grasses (INTER) and overgrown by shrubs and trees (HIGH). In the years 2009–2011 the number of leaf rosettes and length of the longest leaf in rosette (as a measure of allocation in vegetative propagation), as well as height of generative stems, number of capitula per stem and number of seeds per capitulum (as indicators of investment in generative reproduction) were observed. The allocation in vegetative reproduction decreased along the gradient of vegetation height. The gradually decline of number of rosettes might be caused by lack of free space suitable for establishment of new ramets, whereas the small leaf size could be due to drastically augmentation of shading. The allocation in generative reproduction increased along the gradient of vegetation height. The placement of structures necessary for generative propagation in higher layers of herbaceous canopy, as well as considerable number of capitula and seed production might increase the chances for successful recruitment of seedlings. On the basis of obtained results combining with published data it should be assumed, that the substantial allocation in vegetative reproduction and the slight investment in generative propagation seems to be sufficient for persistence of Serratula tinctoria populations in patches dominated by small meadow species, while the considerable allocation in generative reproduction could be crucial for maintenance of population viability under competitive conditions.
The effect of two sterilization substances (calcium hypochlorite and sodium hypochlorite) on the germination rate, and the effect of nitrogen and growth regulators on seedling growth and development, was studied in three critically endangered species of terrestrial orchids (Dactylorhiza incarnata subsp. serotina, Dactylorhiza maculata subsp, maculata, Liparis loeselii). Surface sterilization of mature seeds using 7.2% calcium hypochlorite (until decolorization from brown to ivory) stimulated the germination rate. Addition of peptone at 1 g l-1 concentration or auxin 3-indoleacetic acid (IAA; 1.43 µM) with cytokinin zeatin (0.72 µM) and 1-naphthylacetic acid (NAA; 1.34 µM) to the culture medium significantly increased the growth parameters of seedlings after 12 months.
Defence reactions: 02- generation, superoxide dismutase, catalase, guaiacol peroxidase and ascorbate peroxidase activities after B. cinerea infection in tomato plants propagated in vitro and grown in vivo have been compared. Infection resulted in rapid 02- generation. Superoxide dismutase activity increase was slower than 02- response. In plants propagated in vitro catalase and guaiacol peroxidase activities after infection were induced less strongly than in plants grown in vivo. K2HP04 pretreatment of plants grown in vitro enhanced significantly the activities of catalase and guaiacol peroxidase after infection. Slight restriction of B. cinerea infection development in in vitro propagated plants pretreated with K2HP04 was observed. Porównanie reakcji obronnych po infekcji Botrytis cinerea u roślin pomidora otrzymanych techniką in vitro i rozmnażanych z nasion.
Badania wysiewu i regulacje siewników prowadzono na stanowisku laboratoryjnym, gdzie określono liczbę poprawnie napełnionych otworów, podwójnych napełnień, przepustów oraz parametry podciśnienia i wpływ prędkości obrotowej tarczy na jakość napełniania otworów tarcz nasionami, ustalono też odpowiednie dla wysiewanych nasion wymiary otworów tarcz wysiewających. Po dobraniu parametrów pracy siewnika przeprowadzone będzie badanie wysiewu w warunkach polowych.
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