Wyniki wyszukiwania

1

Role of clathrin- and caveolae-mediated endocytosis in gene transfer mediated by lipo- and polyplexes

100%

Rejman J., Conese M.

Cellular and Molecular Biology Letters

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2005

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tom 10

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nr Suppl.

2

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Primary endosymbiosis: have cyanobacteria and Chlamydiae ever been roommates?

86%

Deschamps P.

Acta Societatis Botanicorum Poloniae

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2014

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tom 83

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nr 4

Eukaryotes acquired the ability to process photosynthesis by engulfing a cyanobacterium and transforming it into a genuine organelle called the plastid. This event, named primary endosymbiosis, occurred once more than a billion years ago, and allowed the emergence of the Archaeplastida, a monophyletic supergroup comprising the green algae and plants, the red algae and the glaucophytes. Of the other known cases of symbiosis between cyanobacteria and eukaryotes, none has achieved a comparable level of cell integration nor reached the same evolutionary and ecological success than primary endosymbiosis did. Reasons for this unique accomplishment are still unknown and difficult to comprehend. The exploration of plant genomes has revealed a considerable amount of genes closely related to homologs of Chlamydiae bacteria, and probably acquired by horizontal gene transfer. Several studies have proposed that these transferred genes, which are mostly involved in the functioning of the plastid, may have helped the settlement of primary endosymbiosis. Some of these studies propose that Chlamydiae and cyanobacterial symbionts coexisted in the eukaryotic host of the primary endosymbiosis, and that Chlamydiae provided solutions for the metabolic symbiosis between the cyanobacterium and the host, ensuring the success of primary endosymbiosis. In this review, I present a reevaluation of the contribution of Chlamydiae genes to the genome of Archaeplastida and discuss the strengths and weaknesses of this tripartite model for primary endosymbiosis.

3

Functional attributes of responding T cells in HCV infection: the recent advances in engineering functional antiviral T cells

86%

Pasetto A., Aleman S., Chen M.

Archivum Immunologiae et Therapiae Experimentalis

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2014

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tom 62

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nr 1

4

Transient expression assay for optimization of direct gene transfer into cucumber meristem protoplasts by electroporation

86%

Burza W., Wochniak P., Wroblewski T., Malepszy S.

Journal of Applied Genetics

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1995

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tom 36

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nr 1

The paper presents a new way of obtaining viable and very homogeneous cucumber protoplasts. Protoplasts from cells formed in the shoot tip meristem culture were isolated from suspension. Plasmid pBI121 was introduced using impulse electric field. Effectiveness of transformation process was determined by the transient expression of ß-glucuronidase (GUS) gene, controlled by promotor 35S. The activity of ß-glucuronidase enzyme as a product of GUS reporter gene was estimated by fluorimetric method (JEFFERSON 1987). Parameters of electroporation process were optimized. The transient expression of GUS gene was measured 24 h after electroporation. The highest effectiveness of transformation process was achieved using three electric impulses at the initial voltage of 250-350 V at 10-sec. intervals as a result of discharging a 140 µF capacitor and 50-70 µg × cm⁻³ plasmid DNA in the presence of 50 µg × cm⁻³ carrier DNA. The system presented is an effective method of exogenic DNA transfer, which is indicated by a high transient expression of the reporter gene. In comparison to Agrobacterium tumefaciens and A. rhizogenes, this alternative method of gene transfer can be used for obtaining transgenic cucumber plants.

5

Gene transfer into the central nervous system using Herpes Simplex Virus-1 vectors

86%

Tabbaa S., Goulah C., Tran R. K., Lis A., Korody R., Stachowski B., Horowitz J. M., Torres G., Stachowiak E. K., Bloom D. C., Stachowiak M. K.

Folia Morphologica

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2000

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tom 59

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nr 4

Manipulation of gene expression in developing or in mature central nervous systems (CNS) holds a promise for the resolution of many compelling neurobiological questions, including the feasibility of gene therapy to treat diseases of the brain. In this context, a number of viral vectors have been used in recent years to introduce and express genes into the CNS. This article discusses a gene transfer system based on the Herpes Simplex Virus-1 (HSV-1). We describe here the use of non-replicating, non-toxic HSV-1 vector, 8117/43, in a series of studies carried in our joint program. This vector proves further the utility of HSV-1 as a delivery vehicle to a number of distinct sites within the CNS.

6

Achradina pulchra, a unique dinoflagellate (Amphilothales, Dinophyceae) with a radiolarian-like endoskeleton of celestite (strontium sulfate)

72%

Gomez F., Kiriakoulakis K., Lara E.

Acta Protozoologica

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2017

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tom 56

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nr 2

7

Plastid origin: who, when and why?

72%

Ku C., Roettger M., Zimorski V., Nelsen-Sathi S., Sousa F. L., Martin W. F.

Acta Societatis Botanicorum Poloniae

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2014

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tom 83

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nr 4

The origin of plastids is best explained by endosymbiotic theory, which dates back to the early 1900s. Three lines of evidence based on protein import machineries and molecular phylogenies of eukaryote (host) and cyanobacterial (endosymbiont) genes point to a single origin of primary plastids, a unique and important event that successfully transferred two photosystems and oxygenic photosynthesis from prokaryotes to eukaryotes. The nature of the cyanobacterial lineage from which plastids originated has been a topic of investigation. Recent studies have focused on the branching position of the plastid lineage in the phylogeny based on cyanobacterial core genes, that is, genes shared by all cyanobacteria and plastids. These studies have delivered conflicting results, however. In addition, the core genes represent only a very small portion of cyanobacterial genomes and may not be a good proxy for the rest of the ancestral plastid genome. Information in plant nuclear genomes, where most genes that entered the eukaryotic lineage through acquisition from the plastid ancestor reside, suggests that heterocyst-forming cyanobacteria in Stanier’s sections IV and V are most similar to the plastid ancestor in terms of gene complement and sequence conservation, which is in agreement with models suggesting an important role of nitrogen fixation in symbioses involving cyanobacteria. Plastid origin is an ancient event that involved a prokaryotic symbiont and a eukaryotic host, organisms with different histories and genome evolutionary processes. The different modes of genome evolution in prokaryotes and eukaryotes bear upon our interpretations of plastid phylogeny.

8

Inhibition of tumor growth by interleukin 10 gene transfer in B16[F10] melanoma cells

72%

Walos S., Szary J., Szala S.

Acta Biochimica Polonica

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1999

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tom 46

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nr 4

Interleukin 10 (IL-10) is a potent immunosuppressive cytokine with an antitumor activity. The effect of IL-10 on tumor growth was tested in murine melanoma cells manipulated by gene transfer to secrete IL-10. In mice bearing B16(F10) tumors expressing IL-10 tumor growth was decreased depending on the amount of secreted IL-10.

9

The effect of TGF-beta1 and Smad7 gene transfer on the phenotypic changes of rat alveolar epithelial cells

72%

Xu G. P., Li Q. Q., Cao X. X., Chen Q., Zhao Z. H., Diao Z. Q., Xu Z. D.

Cellular and Molecular Biology Letters

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2007

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tom 12

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nr 3

The aim of this study was to investigate whether transforming growth factor-β1 (TGF-β1) could induce alveolar epithelial-mesenchymal transition (EMT) in vitro, and whether Smad7 gene transfer could block this transition. We also aimed to elucidate the possible mechanisms of these processes. The Smad7 gene was transfected to the rat type II alveolar epithelial cell line (RLE-6TN). Expression of the EMT-associated markers was assayed by Western Blot and Real-time PCR. Morphological alterations were examined via phase-contrast microscope and fluorescence microscope, while ultrastructural changes were examined via electron microscope. TGF-β1 treatment induced a fibrotic phenotype of RLE-6TN with increased expression of fibronectin (FN), α-smooth muscle actin (α-SMA) and vimentin, and decreased expression of E-cadherin (E-cad) and cytokeratin19 (CK19). After transfecting the RLE-6TN with the Smad7 gene, the expression of the mesenchymal markers was downregulated while that of the epithelial markers was upregulated. TGF-β1 treatment for 48 h resulted in the separation of RLE-6TN from one another and a change into elongated, myofibroblast-like cells. After the RLE-6TN had been transfected with the Smad7 gene, TGF-β1 treatment had no effect on the morphology of the RLE-6TN. TGF-β1 treatment for 48 h resulted in an abundant expression of α-SMA in the RLE-6TN. If the RLE-6TN were transfected with the Smad7 gene, TGF-β1 treatment for 48 h could only induce a low level of α-SMA expression. Furthermore, TGF-β1 treatment for 12 h resulted in the degeneration and swelling of the osmiophilic multilamellar bodies, which were the markers of type II alveolar epithelial cells. TGF-β1 can induce alveolar epithelialmesenchymal transition in vitro, which is dependent on the Smads signaling pathway to a certain extent. Overexpression of the Smad7 gene can partially block this process.

10

Immunotherapy of inflammatory bowel diseases: current concepts and future perspectives

72%

Neurath M. F.

Archivum Immunologiae et Therapiae Experimentalis

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2000

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tom 48

|

nr 2

11

Nonviral transfection of human umbilical cord blood dendritic cells is feasible, but the yield of dentritic cells with transgene expression limits the application of this method in cancer immunotherapy

72%

Markowicz S., Niedzielska J., Kruszewski M., Oldak T., Gajkowska A., Machaj E. K., Skurzak H., Pojda Z.

Acta Biochimica Polonica

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2006

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tom 53

|

nr 1

Dendritic cells (DC) generated from human umbilical cord blood might replace patients' DC in attempts to elicit tumor-specific immune response in cancer patients. We studied the efficiency of transfection of human cord blood DC with plasmid DNA carrying the enhanced version of green fluorescent protein (EGFP) as a reporter gene, to test if nonviral gene transfer would be a method to load DC with protein antigens for immunotherapy purposes. Cord blood mononuclear cells were cultured in serum-free medium in the presence of granulocyte-monocyte colony stimulating factor (GM-CSF), stem cell factor (SCF) and Flt-3 ligand (FL), to generate DC from their precursors, and thereafter transfected by electroporation. Maturation of DC was induced by stimulation with GM-CSF, SCF, FL and phorbol myristate acetate (PMA). Transfected DC strongly expressed EGFP, but transfection efficiency of DC, defined as HLA-DR+ cells lacking lineage-specific markers, did not exceed 2.5%. Expression of the reporter gene was also demonstrated in the DC generated from transfected, purified CD34+ cord blood cells, by stimulation with GM-CSF, SCF, FL, and tumor necrosis factor α (TNF-α). Transfection of CD34+ cells was very efficient, but proliferation of the transfected cells was much reduced as compared to the untransfected cells. Therefore, the yield of transgene-expressing DC was relatively low. In conclusion, nonviral transfection of cord blood DC proved feasible, but considering the requirements for immunotherapy in cancer patients, transfection of differentiated DC or generation of DC from transfected hematopoietic stem cells provide only a limited number of DC expressing the transgene.

12

In vivo gene transfer using cetylated polyethylenimine

72%

Sochanik A., Cichon T., Makselon M., Strozyk M., Smolarczyk R., Jazowiecka-Rakus J., Szala S.

Acta Biochimica Polonica

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2004

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tom 51

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nr 3

This report describes gene transfer in vitro as well as in vivo using cetylated low-molecular mass (600 Da) polyethylenimine (28% of amine groups substituted with cetyl moieties), termed CT-PEI. This compound is hydrophobic and has to be incorporated into liposomes in order to be suitable for gene transfer studies. Serum-induced plasmid DNA degradation assay demonstrated that CT-PEI-containing liposomal carriers could protect complexed DNA (probably via condensation). In vitro lu- ciferase gene expression achieved using medium supplemented with 10% serum was comparable to that achieved in serum-reduced medium and was highest for CT-PEI/cholesterol liposomes, followed by CT-PEI/dioleoylphosphatidylcholine liposomes and PEI 600 Da (uncetylated) carrier. In vivo systemic transfer into mice was most efficient when liposome formulations contained CT-PEI and cholesterol. Higher luciferase expression was then observed in lungs than in liver. In conclusion: liposomes containing cetylated polyethylenimine and cholesterol are a suitable vehicle for investigating systemic plasmid DNA transfer into lungs.

13

Evaluation of the possibility of horizontal gene transfer and accumulation of transgenic DNA from the diet in the bodies of rats

58%

Kosieradzka I., Vasko V., Szwacka M., Przybysz A., Fiedorowicz S.

Journal of Animal and Feed Sciences

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2010

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tom 19

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nr 2

307-315

The procedures for GMO safety tests include traceability of transgenic protein and transgenic DNA if the plant constitutes a component in the diet for an animal. This is due to the possibility of horizontal transfer of genes, accumulation of transgenic DNA in consumer’s organs, or induction of antibiotic resistance in gastrointestinal tract microflora. The last possibility is related to the use of marker genes in the process of transformation. In an in vivo experiment conducted on laboratory rats with the use of transgenic cucumbers expressing the pre-prothaumatin gene, the presence of transgenic DNA in the tissue of kidneys and liver was not detected. Resistance to neomycin of gastrointestinal tract microflora of the rats fed the GMO diet was not found, despite the use of marker genes (npt II) in the process of transformation of the investigated plants.

14

In vivo gene transfer to the brain cortex using a single injection of HSV-1 vector into the medial septum

58%

Tabbaa S., Corso T. D., Jenkins L., Tran R. K., Bloom D. C., Stachowiak M. K.

Folia Morphologica

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2005

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tom 64

|

nr 4

273-281

This study shows that an ICP4– replication-deficient herpes simplex virus containing the Moloney murine leukaemia virus LTR fused with the coding sequence for the β-galactosidase gene can be used as a very effective vector for delivering the β-galactosidase reporter gene into the rat brain septum. F344 rats received bilateral stereotaxic injections into the nucleus of the diagonal band and into the medial septum. The X-gal stain was used to detect the activity of the expressed β-galactosidase enzyme. The delivered reporter gene was expressed successfully not only in the neuronal cells of the injected areas but also in cells that project to the injection area such as cortex cells about 6mm away from the injection sites. Expression was visible at 1, 3 and 9 weeks following injection. We conclude that this vector can effectively deliver genes into different regions of the mature mammalian brain and also to areas distant from the injection site.

15

Insertion of the IL-2 gene decreases tumorigenicity of murine fibrosarcoma

58%

Kusnierczyk H., Zalecki P., Pajtasz-Piasecka E., Radzikowski C.

Archivum Immunologiae et Therapiae Experimentalis

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1998

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tom 46

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nr 4

16

Sequence, structural, and evolutionary analysis of prokaryotic ribosomal protein L11 methyltransferases

58%

Bujnicki J. M.

Acta Microbiologica Polonica

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2000

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tom 49

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nr 1

17

Species from Aegilops genera as the source of tolerance for high aluminium ion concentrations

58%

Stefanowska G., Maslowski J.

Genetica Polonica

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1994

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tom 35B

18

Odpornosc na maczniaka w kolekcji Triticum tauschii Coss.i synteza amfiploidow pomostowych z pszenica heksaploidalna

51%

Sodkiewicz T., Sodkiewicz W., Majewska M.

Zeszyty Problemowe Postępów Nauk Rolniczych

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2007

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tom 517

|

nr 2

885-892

The set consisting of 42 forms of Triticum tauschii (syn. Aegilops squarrosa) Coss. gathered in the National Small Grains Collection (USA) and 4 forms from the collection of the Institute of Plant Genetics PAS was investigated in terms of resistance to powdery mildew (Erysiphe graminis f. sp. tritici) under the artificial inoculation conditions. Out of all studied forms, 21 collection forms (45.6%) expressed resistance at the seedling stage and at the stage of vegetative development, but only in 12 forms (26%) resistance was expressed at the fully developed stage. Resistant forms formed clusters in terms of their origin from specific geographical regions. Under the infection promoting conditions, hexaploid wheat cultivars Igna, Omega and Tercja were already distinctly infected at the early development stages and were strongly infected at the fully developed stage. Two completely resistant forms of T. tauschii Coss. (151 PI, 152 PI) were crossed with a susceptible wheat cultivar Igna to introduce genetic material directly from genome Dt carrying resistance genes (omitting the generation of a synthetic hexaploid). The previously described crossing efficiency using T. tauschii Coss. as a maternal form was confirmed. Embryos from the non-endospermic hybrid cary-opses were cultured in vitro. The character limiting the potential of generating F₁ plants was high susceptibility of embryos to callus formation even at their considerably advanced developmental stages. Out of the produced F₁ plants as a result of the colchicine treatment allooctoploid genotypes were generated with the ABBDDDtDt genome, which may be used as bridging forms for the introgression of resistance genes into selected wheat cultivars.

19

Applications of recent advances at the Institute of Grassland and Environmental Research in cytogenetics of the Lolium-Festuca complex

51%

Humphreys M. W., Thomas H. M., King I. P., Morgan W. G., Meredith M. R., Harper J. A., Humphreys M. O., Bettany A. J. E., Dalton S. J., James A. R., Ougham H. J., Thomas H.

Journal of Applied Genetics

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1997

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tom 38

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nr 3

Recent advances at Institute of Grassland and Environmental Research (Aberystwyth, U.K.) in cytogenetics of the Lolium/Festuca complex places us in the advantageous position of being able to map genes of agronomic importance onto chromosome arms using fluorescence in situ hybridization (FISH). The ability to physically map genes leads to the capability for "dissecting" quantitative traits into their different components and will lead to better understanding of the complex physiological processes involved and the identification of their genetic control. By tagging genes of interest, using molecular and morphological markers, it will be possible to select and combine suites of desirable genes in a single genotype and thus produce novel cultivars by conventional breeding procedures. Programmes for introgression depend on the relationships between species and on levels of chromosome pairing. Phylogenetic relationships within the Lolium/Festuca complex are being determined using both genomic in situ hybridization (GISH) and FISH. With recent advances in genetic manipulation within the Lolium/Festuca complex, opportunities now arise for gene transfer from Lolium and Festuca species into other important agricultural crops.

20

The dynamic nature of plant mitochondrial genome organization

51%

Janska H., Woloszynska M.

Acta Biochimica Polonica

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1997

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tom 44

|

nr 2

The characteristic features of higher plant mitochondrial genomes: size, structure, recombination activity and evolutionary dynamics, are reviewed with the emphasis on the mitochondrial DNA (mtDNA) of Phaseolus vulgaris. Among all examined eukaryotic organisms, higher plants were found to contain the largest and most complex mitochondrial genomes. The plant mtDNA structure in vivo and mechanisms of evolution are controversial. We present the currently accepted models and how these models correspond to mitochondrial genomes of several common bean lines.

Ograniczanie wyników

Role of clathrin- and caveolae-mediated endocytosis in gene transfer mediated by lipo- and polyplexes

Primary endosymbiosis: have cyanobacteria and Chlamydiae ever been roommates?

Functional attributes of responding T cells in HCV infection: the recent advances in engineering functional antiviral T cells

Transient expression assay for optimization of direct gene transfer into cucumber meristem protoplasts by electroporation

Gene transfer into the central nervous system using Herpes Simplex Virus-1 vectors

Achradina pulchra, a unique dinoflagellate (Amphilothales, Dinophyceae) with a radiolarian-like endoskeleton of celestite (strontium sulfate)

Plastid origin: who, when and why?

Inhibition of tumor growth by interleukin 10 gene transfer in B16[F10] melanoma cells

The effect of TGF-beta1 and Smad7 gene transfer on the phenotypic changes of rat alveolar epithelial cells

Immunotherapy of inflammatory bowel diseases: current concepts and future perspectives

Nonviral transfection of human umbilical cord blood dendritic cells is feasible, but the yield of dentritic cells with transgene expression limits the application of this method in cancer immunotherapy

In vivo gene transfer using cetylated polyethylenimine

Evaluation of the possibility of horizontal gene transfer and accumulation of transgenic DNA from the diet in the bodies of rats

In vivo gene transfer to the brain cortex using a single injection of HSV-1 vector into the medial septum

Insertion of the IL-2 gene decreases tumorigenicity of murine fibrosarcoma

Sequence, structural, and evolutionary analysis of prokaryotic ribosomal protein L11 methyltransferases

Species from Aegilops genera as the source of tolerance for high aluminium ion concentrations

Odpornosc na maczniaka w kolekcji Triticum tauschii Coss.i synteza amfiploidow pomostowych z pszenica heksaploidalna

Applications of recent advances at the Institute of Grassland and Environmental Research in cytogenetics of the Lolium-Festuca complex

The dynamic nature of plant mitochondrial genome organization