Wyniki wyszukiwania

1

Epigenetic gene silencing in Arabidopsis: genetic triggers and molecular consequences

100%

Mittelsten Scheid O., Paszkowski J.

Journal of Applied Genetics

|

1996

|

tom 37A

2

The ts111 mutation of Paramecium tetraurelia affects a member of the protein palmitoylation family

86%

Prajer M., Tarcz S.

Folia Biologica

|

2015

|

tom 63

|

nr 3

3

Effects of alpha-enolase gene silencing on reproductive-related hormone receptor expression and steroid hormone synthesis of primary granulosa cells from goose F1 follicles

86%

Ji H., Niu C.-Y., Zhang H.-L., Guo J. R., Zhen L., Lian S., Yang C., Yang H.-M., Wang J.-F.

Journal of Veterinary Research

|

2020

|

tom 64

|

nr 1

4

Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik

Changes in DNA methylation in maize under herbicide stress conditions

86%

Tyczewska A., Gracz J., Szymkowiak J., Twardowski T.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

|

2015

|

tom 96

|

nr 1

5

Selected technologies to control genes and their products for experimental and clinical purposess

86%

Alexander H. K., Booy E. P., Xiao W., Ezzati P., Baust H., Los M.

Archivum Immunologiae et Therapiae Experimentalis

|

2007

|

tom 55

|

nr 3

6

RNA interference and its role in the regulation of eukaryotic gene expression

86%

Szweykowska-Kulinska Z., Jarmolowski A., Figlerowicz M.

Acta Biochimica Polonica

|

2003

|

tom 50

|

nr 1

Several years ago it was discovered that plant transformation with a transcribed sense transgene could shut down the expression of a homologous endogenous gene. Moreover, it was shown that the introduction into the cell of dsRNA (double-stranded RNA) containing nucleotide sequence complementary to an mRNA sequence causes selective degradation of the latter and thus silencing of a specific gene. This phenomenon, called RNA interference (RNAi) was demonstrated to be present in almost all eukaryotic organisms. RNAi is also capable of silencing transposons in germ line cells and fighting RNA virus infection. Enzymes involved in this process exhibit high homology across species. Some of these enzymes are involved in other cellular processes, for instance developmental timing, suggesting strong interconnections between RNAi and other metabolic pathways. RNAi is probably an ancient mechanism that evolved to protect eukaryotic cells against invasive forms of nucleic acids.

7

Interference of nematode parasitism by silencing of plant genes

72%

Koter M., Matuszkiewicz M., Baranowski L., Sobczak M., Wisniewska A., Dabrowska-Bronk J., Swiecicka M., Skowron W., Filipecki M.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

|

2013

|

tom 94

|

nr 3

8

Can CYP1A1 siRNA be an effective treatment for lung cancer?

72%

Mohammed K., Shervington A.

Cellular and Molecular Biology Letters

|

2008

|

tom 13

|

nr 2

240-249

Abstract: Previously, we identified a novel correlation between the upregulated expression of telomerase (hTERT) and cytochrome P450 1A1 (CYP1A1) in A549 human lung cancer cell line. The expression correlation was confirmed by silencing CYP1A1 expression using siRNA technology and observing a silencing of hTERT transcription. Furthermore, silencing CYP1A1 and subsequently downregulating hTERT resulted in the reduction of cancer cell viability by more than 40%, which appeared as early as 24 hours after the treatment. The concomitant downregulation of CYP1A1 and hTERT resulted in rapid cell death. This finding can be further exploited to develop new molecular targets for the treatment of lung cancer.

9

RNA interference: a new strategy in the evolutionary arms race between human control strategies and insect pests

72%

Machado V., Rodriguez-Garcia M. J., Sanchez-Garcia F. J., Galian J.

Folia Biologica

|

2014

|

tom 62

|

nr 4

10

A study on the fundamental factors determining the efficacy of siRNAs with high C-G contents

72%

Liao J. Y., Yin J. Q., Chen F., Liu T.-G., Yue J.-C.

Cellular and Molecular Biology Letters

|

2008

|

tom 13

|

nr 2

283-302

Although there are many reports about the efficacy of siRNAs, it is not clear whether those siRNAs with high C/G contents can be used to silence their target mRNAs efficiently. In this study, we investigated the structure and function of a group of siRNAs with high C/G contents. The results showed that single siRNAs against the Calpain, Otoferlin and Her2 mRNAs could induce different silencing effects on their targets, suggesting that the accessibility to target sequences influences the efficacy of siRNA. Unexpectedly, a single siRNA could target its cognate sequence in the 3’UTR of EEF1D or the 5’UTR of hTRF2 or CDC6. Their interaction induced different modes of gene silencing. Furthermore, the introduction of mutations into the 3’ end of the passenger strand showed that the position and number of mutated nucleotides could exert some influence on the efficacy of siRNA. However, these mutations did not completely block the passenger strand from exerting its RNAi effect. Interestingly, our findings also indicated that the target mRNA might play essential roles in maintaining or discarding the guide strand in RISCs. Thus, the conclusion could be drawn that favorable siRNA sequences, accessible target structures and the fast cleavage mode are necessary and sufficient prerequisites for efficient RNAi.

11

Celastrol overcomes Hsp72 gene silencing-mediated muscle atrophy and induces myofiber preservation

72%

Gwag T., Park K., Park J., Lee J.-H., Nikawa T., Choi I.

Journal of Physiology and Pharmacology

|

2015

|

tom 66

|

nr 2

12

MiRNA - a novel functional component of food?

72%

Wojciechowska K.

Towaroznawcze Problemy Jakości. Polskie Towarzystwo Towaroznawcze

|

2013

|

nr 2

13

DNA methylation and regulation of transcriptional gene silencing in Arabidopsis

72%

Paszkowski J.

Biological Letters

|

2005

|

tom 42

|

nr 2 Spec.Vol.

14

Brome mosaic virus [BMV] and barley stripe mosaic virus [BSMV] - based vectors: application in gene silencing in plants

72%

Barciszewska-Pacak M., Pacak A., Florek B., Alejska M., Lacomme C., Szweykowska-Kulinska Z., Figlerowicz M., Jarmolowski A.

Biological Letters

|

2005

|

tom 42

|

nr 2 Spec.Vol.

15

Transgenes in plants: protection against viruses and insects

72%

Augustyniak J., Dabert M., Wypijewski K.

Acta Physiologiae Plantarum

|

1997

|

tom 19

|

nr 4

Protective transgenes introduced into plants can be classified as directed against insects, fungi, bacteria or viruses. Mechanisms by which they protect plants in some cases are relatively simple and understood while in most cases they present only the field of rapidly progressing investigations. A brief review of the recent concepts of the resistance induced in plants against viruses by virus-derived transgenes is presented with emphasising the RNA mediated resistance. The RNA mediated resistance seems to operate in Nicotiana benthamiana plants transformed in our laboratory with cDNA of the PPV CP gene: both translatable and untranslatable versions of the cDNA made the transformed plants resistant against PPV. The resistant plants contained more than one copy of the transgene. To protect against insects plants were in our laboratory transformed with potato proteinase inhibitor II gene (PPI-II). The PPI-II gene expressed in model plants inhibited trypsin activity to an expected level.

16

siRNAs and miRNAs: small RNA molecules for big tasks

72%

Lesicka J., Piontek P., Szweykowska-Kulinska Z., Jarmolowski A.

Acta Physiologiae Plantarum

|

2004

|

tom 26

|

nr 3

17

Structural and functional characteristics of two novel members of pathogenesis-related multigene family of class from yellow lupine

58%

Handschuh L., Femiak I., Kasperska A., Figlerowicz M., Sikorski M. M.

Acta Biochimica Polonica

|

2007

|

tom 54

|

nr 4

783-796

PR-10 proteins (pathogensis-related), ubiquitous within the plant kingdom, are usually encoded by multigene families. To date we have identified 10 homologous pr-10 genes in a yellow lupine cDNA library. Here, the structure and expression of two newly identified yellow lupine pr-10 genes (LlYpr10-2b and LlYpr10-2f) are presented. Many potential regulatory sites were found in both gene promoters including common ones as well as those unique for each gene. However, promoter deletion analysis in transgenic tobacco plants revealed similar patterns of reporter gene (gus) expression. Shortened fragments of both gene promoters studied caused high GUS activity in leaves (along vascular bundles), stamen stigma, anthers and pollen grains. When conjugated with longer LlYpr-10.2 promoter fragments, GUS was additionally present in petal edges. Only a long fragment of the LlYpr10-2b gene promoter caused GUS expression in the stem. In yellow lupine the pr-10.2 genes are present in all studied organs, but their level of expression depends on the stage of development and is affected by wounding, oxidative stress and salicylic acid treatment. Silencing of the Llpr-10.2b gene in 4-week-old yellow lupine plants did not lead to any visible symptoms, which suggests that the function of the silenced gene is supplemented by its close homologues, still present in the studied plants.

18

A new BSMV-based vector with modified beta molecule allows simultaneous and stable silencing of two genes

58%

Kawalek A., Dmochowska-Boguta M., Nadolska-Orczyk A., Orczyk W.

Cellular and Molecular Biology Letters

|

2012

|

tom 17

|

nr 1

Virus-induced gene silencing is an important tool for functional gene analysis and the vector based on Barley stripe mosaic virus (BSMV) is widely used for the purpose in monocots. Of the tripartite BSMV genome, currently the BSMV:γMCS molecule is used to clone a fragment of a target gene. As an alternative, the BSMV:β molecule was engineered with a unique BamHI site between the open reading frame of βc (ORF βc) and poly(A). The mixture of RNA particles α, βBamHI and γMCS was fully infectious. Barley phytoene desaturase and wheat phospholipase Dα fragments were cloned to βBamHI and γMCS. Delivery of the target gene fragment in γMCS induced stronger silencing, while delivery in βBamHI yielded more stable transcript reduction. A quantitative analysis (qRT-PCR) of the transcripts showed that the silencing induced with a fragment carried in both particles was stronger and more stable than that from a fragment placed in one particle. The modification of β enables simultaneous silencing of two genes. Quantifying the β and γ particles in virus-inoculated plants revealed a 2.5-fold higher level of γ than β, while the stability of the insert was higher in β compared with γ. The possible influence of the relative quantity of β and γ particles in virus-inoculated plants on insert stability and gene silencing efficiency is discussed.

19

RNAi-mediated gene silencing in legumes

58%

Handschuh L., Florek B., Sikorski M. M., Figlerowicz M.

Biological Letters

|

2005

|

tom 42

|

nr 2 Spec.Vol.

Ograniczanie wyników

Epigenetic gene silencing in Arabidopsis: genetic triggers and molecular consequences

The ts111 mutation of Paramecium tetraurelia affects a member of the protein palmitoylation family

Effects of alpha-enolase gene silencing on reproductive-related hormone receptor expression and steroid hormone synthesis of primary granulosa cells from goose F1 follicles

Changes in DNA methylation in maize under herbicide stress conditions

Selected technologies to control genes and their products for experimental and clinical purposess

RNA interference and its role in the regulation of eukaryotic gene expression

Interference of nematode parasitism by silencing of plant genes

Can CYP1A1 siRNA be an effective treatment for lung cancer?

RNA interference: a new strategy in the evolutionary arms race between human control strategies and insect pests

A study on the fundamental factors determining the efficacy of siRNAs with high C-G contents

Celastrol overcomes Hsp72 gene silencing-mediated muscle atrophy and induces myofiber preservation

MiRNA - a novel functional component of food?

DNA methylation and regulation of transcriptional gene silencing in Arabidopsis

Brome mosaic virus [BMV] and barley stripe mosaic virus [BSMV] - based vectors: application in gene silencing in plants

Transgenes in plants: protection against viruses and insects

siRNAs and miRNAs: small RNA molecules for big tasks

Structural and functional characteristics of two novel members of pathogenesis-related multigene family of class from yellow lupine

A new BSMV-based vector with modified beta molecule allows simultaneous and stable silencing of two genes

RNAi-mediated gene silencing in legumes