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Modified procedure for PCR detection of proviral DNA of bovine leukaemia virus

100%

Czarnik U., Kaminski S., Rulka J., Walawski K.

Bulletin of the Veterinary Institute in Puławy

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2000

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tom 44

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nr 2

PCR modification was performed by using two sets of primers specific for BLV gag gene and bovine kappa-casein gene (CASK) which show the possibility of disclosing BLV misdiagnosis. The results obtained indicate that the described PCR modification can be proposed as a simple and reliable method of BLV provirus detection.

2

Real defect of AMPD1 accompanied with myopathy: new missense mutations found in a Japanese patient

84%

Morisaki T., Morisaki H., Higuchi I., Osame M., Abe M.

Cellular and Molecular Biology Letters

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1999

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tom 04

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nr 3

3

Respiratory dysfunction in patients with Marfan Syndrome

67%

Baran S., Ignys A., Ignys I.

Journal of Physiology and Pharmacology. Supplement

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2007

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tom 58

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nr 5

4

Ghrelin gene products in acute and chronic inflammation

67%

Prodam F., Filigheddu N.

Archivum Immunologiae et Therapiae Experimentalis

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2014

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tom 62

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nr 5

5

Regulating cell division events during male gametophyte development

67%

Twell D., Oh S.-A., Durbarry A., Johnson A., Wardle T., Smertenko A., Hussey P., Rotman N., Faure J.-E., Berger F.

Acta Biologica Cracoviensia. Series Botanica. Supplement

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2005

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tom 47

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nr 1

6

Characteristics of gene 28 product, the constituent of the central part of bacteriophage T4 baseplate

67%

Nieradko J., Koszalka P., Krzywicka A.

Acta Microbiologica Polonica

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1998

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tom 47

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nr 3

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Molecular cloning and characterisation of phosphoglycerate kinase from Fasciola hepatica

51%

Jaros S., Wesolowska A., Jaros D., Zygner W., Wedrychowicz H.

Bulletin of the Veterinary Institute in Puławy

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2011

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tom 55

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nr 3

This paper is focused on cloning and bioinformatical as well as immunological characterisation of the new vaccine antigen candidate against fasciolosis - Fasciola hepatica phosphoglycerate kinase (FhPGK). The antigen was cloned from the adult fluke by the use of RACE-PCR method. It was found that FhPGK is not a secretory and not a stage specific protein. It is present in all kinds of parasite tissue, particularly in fluke intestine and tegumental as well as subtegumental layers. FhPGK is involved in production of the first ATP molecule in the glycolytic pathway and can be used in vaccination trials in which the strategy is to block fluke's energy metabolism. This is the first, to date, phosphoglycerate kinase cloned from F. hepatica.

Ograniczanie wyników

Modified procedure for PCR detection of proviral DNA of bovine leukaemia virus

Real defect of AMPD1 accompanied with myopathy: new missense mutations found in a Japanese patient

Respiratory dysfunction in patients with Marfan Syndrome

Ghrelin gene products in acute and chronic inflammation

Regulating cell division events during male gametophyte development

Characteristics of gene 28 product, the constituent of the central part of bacteriophage T4 baseplate

Molecular cloning and characterisation of phosphoglycerate kinase from Fasciola hepatica