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Physical mapping of genes by fluorescence in situ hybridization (FISH) seems to be out of fashion in species whose assembled genome sequences are available. However, in this work we evidence the existence of errors in gene location in the Btau_4.0 assembly. We show that DFNA5 and CHCHD6 genes are located on BTA4 and BTA22, respectively, instead of BTA10 and BTA3, as displayed by Btau_4.0. This report emphasizes the need to verify the data on physical localization of genes in the cattle genome (at least by taking into account comparative data reported in available papers) and the need to improve the cattle genome assembly. Our results indicate that FISH mapping in cattle is still useful.
R-spondins constitute a recently discovered small family of growth factors, and the evidence of their role in several developmental pathways is growing fast. In this work we describe the chromosomal location of the four RSPO genes in the donkey. Using horse BACs, we localized RSIPO1 on EAS 5q23, RSPO2 on EAS 12q13, RSPO3 on EAS 24q26, and RSPO4 on EAS 15p13. Moreover, RSPO2, RSPO3, and RSPO4 are the first genes mapped on donkey chromosomes 12, 24, and 15, respectively.
Expressed Sequence Tags (ESTs) are short, usually unedited sequences obtained by single-pass sequencing of cDNA clones from any cDNA library. Analyzing and comparing ESTs can provide information on gene expression, function and evolution. Large-scale EST sequencing has become an attractive alternative to plant genome sequencing. Currently, plant EST coltections comprise over 3.8 miliion sequences from about 200 species. They have proved to be a valuable tool for gene discovery and plant metabolism analysis. Several plant-specific EST databases have been created which provide access to sequence data and bioinformatics-based tools for data mining. Searching EST coltections allows pre-selection of genes for preparing cDNA arrays, targeted to bring maximum information on specialized processes, like stress response, symbiotic nitrogen fixation etc. Also, EST-based molecular markers such as SNP, SSR, and indels are fast developing tools for breeders and re searchers.
The search for genes with that positively affect physical fitness is a difficult process. Physical fitness is determined by numerous genes, and its genetic determinants are modified by environmental factors. The map of candidate genes that can potentially affect physical fitness becomes larger every year, and currently it contains more than 200 genes associated with such aspects as respiratory and cardiovascular stability; body build and composition – especially muscle mass and strength; carbohydrate and lipid metabolism; response to training; and exercise intolerance. The inclusion of the genetic component in physiological and biochemical studies would permit drawing a representation of predispositions for each athlete interested in practicing high performance sports and would be a valuable coaching aid in the process of training individualization.
Location of the loci that control preharvest sprouting and alpha-amylase activity in rye was studied based on intercross S120×S76, consisting of 110 genotypes of F2 and F3 progenies. The genetic map currently consists of 141 loci distributed in 11 linkage groups, covering a distance of 506.4 cM, and was enriched during this study with 24 sequence-specific markers (7 SCARs, 7 SSRs, and 10 STSs). The extended map was applied for composite interval mapping of the loci controlling preharvest sprouting and a-amylase activity, revealing 3 significant QTLs for preharvest sprouting, located on chromosomes 3R, 5R and 6R (in 1999), and one QTL for a-amylase activity found on chromosome 2R (in 2000).
W pracy przedstawione są wstępne wyniki badań prowadzonych na podstawie linii uzyskanych z kombinacji krzyżówkowych Wt11238 × Wt3557 i Wt10245 × Wt11238 grochu Pisum sativum L., zmierzających do opisu działania loci determinujących cechy związane z plonowaniem. Podane są rezultaty doświadczenia jednorocznego prowadzonego w dwu środowiskach. Opisana jest zastosowana metoda statystyczna mapowania loci cech ilościowych, która uwzględnia sposób otrzymania linii i pozwala na ocenę efektów addytywnego działania genów oraz dominacji.
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