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The role of plasminogen activation system in cancer cell biology

100%

Zekanowska E. B.

Acta Biochimica Polonica

2007

tom 54

nr Supplement 4

Decreased expression of the human carbonyl reductase 2 gene HCR2 in hepatocellular carcinoma

84%

Liu S., Ma L., Huang W., Shai Y., Ji X., Ding L., Liu Y., Yu L., Zhao S.

Cellular and Molecular Biology Letters

2006

tom 11

nr 2

Altered gene expression was associated with the induction and maintenance of hepatocellular carcinoma (HCC). To determine the significance of HCR2 in HCC, here we compare the expression levels of HCR2 in carcinoma and in paired non-carcinoma tissues using semiquantitative reverse-transcription polymerase chain reaction (RT-PCR), Western blot analysis, and immunohistochemical staining. The expression ratio (ER) of HCR2 between the tumor and paired tumor-free tissues was calculated for each case and the data was clinicopathologically analyzed. The expression of HCR2 mRNA was found to be significantly decreased in HCC tissues compared with paired normal tissues (P < 0.001). HCR2 was downregulated in 58% (n = 22) of 38 HCC patients. The ER of HCR2 was higher in Edmondson’s grade I/II carcinomas than that in Edmondson’s grade III/IV carcinomas (P < 0.05). Western blot analysis showed HCR2 to be notably depressed in carcinoma tissues in 3 out of 4 HCC patients. Immunohistochemical staining indicated most HCR2 protein accumulated in non-carcinoma cells. These results suggested that altered HCR2 expression might play roles in the carcinogenesis and progression of HCC, and it could be a clinical marker for prognosis, and a molecular target for screening potential anti-HCC drugs.

Quantitative analysis of the level of p53 and p21 WAF1 mRNA in human colon cancer HT-29 cells treated with inositol hexaphosphate

67%

Weglarz L., Molin I., Orchel A., Parfiniewicz B., Dzierzewicz Z.

Acta Biochimica Polonica

2006

tom 53

nr 2

The aim of this study was to analyze the molecular mechanism of inositol hexaphosphate (InsP6) action through which it may inhibit proliferation of colon cancer cells and cell cycle progression. A kinetic study of p53 and p21WAF1 mRNA increase was performed on human colon cancer HT-29 cells after treatment with 1, 5 and 10 mM InsP6 for 6, 12, 24 and 48 h. Real-time-QPCR based on TaqMan methodology was applied to analyze quantitatively the transcript levels of these genes. The transcription of β-actin and GAPDH genes was assessed in parallel to select the control gene with least variability. The 2-ΔΔCt method was used to analyze the relative changes in gene transcription. InsP6 stimulated p53 and p21WAF1 expression at the mRNA level, with the highest increase in p21WAF1 mRNA occurring at 24 h, i.e., following the highest increase in p53 mRNA observed at 12 h. Based on these studies it may be concluded that the ability of InsP6 to arrest the cell cycle may be mediated by the transcriptional up-regulation of the p53-responsive p21WAF1 gene.

Ograniczanie wyników

2 Acta Biochimica Polonica

1 Cellular and Molecular Biology Letters

1 Ding L.

1 Dzierzewicz Z.

1 Huang W.

1 Ji X.

1 Liu S.

1 Liu Y.

1 Ma L.

1 Molin I.

1 Orchel A.

1 Parfiniewicz B.

1 Shai Y.

1 Weglarz L.

1 Yu L.

1 Zekanowska E. B.

1 Zhao S.

1 2007

2 2006

Wyniki wyszukiwania

The role of plasminogen activation system in cancer cell biology

Decreased expression of the human carbonyl reductase 2 gene HCR2 in hepatocellular carcinoma

Quantitative analysis of the level of p53 and p21 WAF1 mRNA in human colon cancer HT-29 cells treated with inositol hexaphosphate