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1

Carrier-state of D allele in ACE gene insertion deletion polymorphism is associated with coronary artery disease, in contrast to the C677-T transition in the MTHFR gene

100%
Zak I., Niemiec P., Sarecka B., Balcerzyk A., Ciemniewski Z., Rudowska E., Dylag S.
Acta Biochimica Polonica
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2003
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tom 50
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nr 2
Angiotensin I-converting enzyme (ACE), which plays an important role in blood pressure regulation, and methylenetetrahydrofolate reductase (MTHFR) involved in homocysteine metabolism belong to a large group of polypeptides which may be po­tential risk factors for atherosclerosis and coronary artery disease (CAD). To assess whether polymorphisms of the genes encoding these peptides are associated with CAD in Silesian we conducted a study among 68 individuals suffering from CAD (in­cluding 52 cases after myocardial infarction), 51 subjects with positive family history of CAD and 111 controls. We analysed the distribution of genotypes and allele fre­quencies of the insertion/deletion (I/D) polymorphism in the ACE gene using PCR am­plification, and the C677^T polymorphism in the MTHFR gene using PCR-RFLP analysis. We found that D allele frequency was significantly higher in CAD patients (61%) than in controls (43%) (P = 0.001, OR = 2.06). The D allele carriers (DD + ID geno­types) were more frequent in the CAD patients (85%) compared to control group (65%) (P = 0.003, OR = 3.14), whereas the familial CAD risk group shows the highest frequency of the ID genotype (57% vs 43% in controls). In contrast, the MTHFR polymorphism does not seem to be associated with the disease. Our data indicate that in Silesian CAD patients the disease is strongly associated with carrier-state of the ACE D allele, but not with the C677->T transition in the MTHFR gene.
2
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The angiotensin converting enzyme gene I/D polymorphism in Polish rowers

84%
Jastrzebski Z., Leonska-Duniec A., Kolbowicz M., Tomiak T.
Central European Journal of Sport Sciences and Medicine
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2014
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tom 05
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nr 1
Angiotensin converting enzyme gene (ACE) is the most frequently investigated genetic marker in the context of genetic conditioning of athletic predispositions. The product of the gene is a key component of the renin-angiotensin system (RAS) and the kallikrein-kinin system (KKS), mainly responsible for the regulation of blood pressure. The main aim of the study was to determine the possible interaction between the ACE l/D polymorphism and endurance athlete status in a group of Polish rowers in comparison with sedentary individuals. 121 male Polish rowers, members of academic sports clubs, and 115 unrelated volunteers, were recruited for the study. The PCR amplification of the insertion (I) or deletion (D) fragment of the ACEgene was performed. Compared with control group, the frequency of the I allele differ significantly from that found in rowers (57.4% vs. 44.3%; P = 0.013) and the ACE genotype frequency amongst the whole cohort of rowers (30.6% II, 53.7% ID, 15.7% DD) was also different from expected values (control group 19.1% II, 50.4% ID, 30.4% DD; P=0.017). Our investigation confirms a positive association of the I allele of the ACE gene with endurance athlete status in a group of Polish rowers.
3

Gene insertion into strawberry leaf disk regeneration system using particle bombardment

67%
Sawahel W., Saker M.
Cellular and Molecular Biology Letters
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1997
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tom 02
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nr 3
Strawberry has previously been transformed using Agrobacterium-mediated DNA transfer. In this paper, we present a process for delivering foreign genes into strawberry leaf disks using particle bombardment. Expression of foreign DNA into strawberry leaf disks (Fragaria X ananassa Duch.) was detected - using in situ GUS assay-following bombardment with tungsten particles coated with plasmid DNA (pBI221.23) that coded for the selectable (hygromycin phosphotransferase [hpt] and the screenable (β-glucuronidase [GUS]) marker genes. Both genes are under the control of the Cauliflower Mosaic Virus (CaMV) 35S promoter. The criteria of stability of phenotypes after the removal of selective pressure, Southern blot hybridization and segregation analysis were used to confirm the mitotic stability of the foreign gene and its stable integration into the strawberry genome progeny. The relative simplicity of this system recommends its future use for the production of genetically modified strawberry.
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