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Gastric mucin secretion in response to beta-adrenergic G protein-coupled receptor activation is mediated by SRC kinase-dependent epidermal growth factor receptor transactivation

100%

Slomiany B. L., Slomiany A.

Journal of Physiology and Pharmacology

2005

tom 56

nr 2

In many systems, the integration of converging regulatory signals that relay on G protein-coupled receptor (GPCR) activation into functional cellular pathways requires the involvement of receptor tyrosine kinase. In this report, we provide evidence that activation of GPCR by ß-adrenergic agonist leading to stimulation in gastric mucin secretion requires epidermal growth factor receptor (EGFR) participation. Using [3H]glucosamine-labeled gastric mucosal cells, we show that stimulatory effect of ß-adrenergic agonist, isoproterenol, on mucin secretion was inhibited by EGFR kinase inhibitor, PD153035, as well as wortmannin, a specific inhibitor of PI3K. Both inhibitors, moreover, blunted the mucin secretory responses to ß-adrenergic agonist-generated second messenger, cAMP as well as adenylate cyclase activator, forskolin. The gastric mucin secretory responses to isoproterenol, furthermore, were inhibited by PP2, a selective inhibitor of tyrosine kinase Src responsible for ligand-independent EGFR autophosphorylation, but not by ERK inhibitor, PD98059. The inhibition of ERK, moreover, did not cause attenuation in mucin secretion in response to cAMP and forskolin. The findings underline the role of EGFR as a convergence point in gastric mucin secretion triggered by ß-adrenergic GPCR activation, and demonstrate the requirement for Src kinase in EGFR transactivation.

Application of aqueous hydrazine solution for beta-elimination of O-glycans from gastric mucin

86%

Kisiel D., Gindzienski A., Gadek A.

Acta Biochimica Polonica

1999

tom 46

nr 3

O-Glycans from pig gastric mucin were released by β-elimination in 0.2 M triethylamine and 50% aqueous hydrazine water solution. The released glycan hydrazides were isolated using Centricon 10 separators, brought to their reducing form and reductive by labelled with p-aminobenzoic acid ethyl ester (ABEE). Labelled products were fractionated into neutral and acid fractions on a Bio-Gel P4 column, calibrated with a mixture of dextran oligosaccharides, labelled according to the same procedure.

Dietary threonine prevented stress-related mucosal diseases in rats

58%

An J. M., Kang E. A., Han Y. M., Kim Y. S., Hong Y. G., Hah B. S., Hong S. P., Hahm K. B.

Journal of Physiology and Pharmacology

2019

tom 70

nr 3

Ograniczanie wyników

2 Journal of Physiology and Pharmacology

1 Acta Biochimica Polonica

1 An J. M.

1 Gadek A.

1 Gindzienski A.

1 Hah B. S.

1 Hahm K. B.

1 Han Y. M.

1 Hong S. P.

1 Hong Y. G.

1 Kang E. A.

1 Kim Y. S.

1 Kisiel D.

1 Slomiany A.

1 Slomiany B. L.

1 2019

1 2005

1 1999

Wyniki wyszukiwania

Gastric mucin secretion in response to beta-adrenergic G protein-coupled receptor activation is mediated by SRC kinase-dependent epidermal growth factor receptor transactivation

Application of aqueous hydrazine solution for beta-elimination of O-glycans from gastric mucin

Dietary threonine prevented stress-related mucosal diseases in rats