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Identyfication of Staphylococcus spp. strains isolated from milk samples of mastitic cows by PCR-RFLP analysis of gap gene

100%

Karakulska J., Sawicka I.

Advances in Agricultural Sciences

2008

tom 12

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Molecular typing of Staphylococcus aureus based on PCR-RFLP of coa gene and RAPD analysis

100%

Karakulska J., Pobucewicz A., Nawrotek P., Muszynska M., Furowicz A. J., Czernomysy-Furowicz D.

Polish Journal of Veterinary Sciences

2011

tom 14

nr 2

The aim of this study was molecular identification of S. aureus strains isolated from mastitic milk samples and establishing the genetic relationship between strains isolated from cows belonging to the same herd. In all 43 isolated strains the gap gene (930 bp) was amplified, which enabled their affiliation to the Staphylococcus genus to be established. PCR-RFLP with AluI endonuclease of the gap gene as well as nuc (450 bp) and coa (1130 bp) gene amplification allowed precise S. aureus species identification. One hundred percent of the genetic relationship between strains was established via RAPD-PCR and coa-typing.

Ograniczanie wyników

1 Advances in Agricultural Sciences

1 Polish Journal of Veterinary Sciences

2 Karakulska J.

1 Czernomysy-Furowicz D.

1 Furowicz A. J.

1 Muszynska M.

1 Nawrotek P.

1 Pobucewicz A.

1 Sawicka I.

1 2011

1 2008

Wyniki wyszukiwania

Identyfication of Staphylococcus spp. strains isolated from milk samples of mastitic cows by PCR-RFLP analysis of gap gene

Molecular typing of Staphylococcus aureus based on PCR-RFLP of coa gene and RAPD analysis