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Physico-chemical characteristics of different genetic variants of bovine beta-casein, modified covalently by glucose, galactose and lactose

100%

Dziuba J., Darewicz M., Mioduszewska H.

Polish Journal of Food and Nutrition Sciences

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1998

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tom 07

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nr 2 Suppl.

2

Characterization of bovine serum albumin glycated with glucose, galactose and lactose

84%

Ledesma-Osuna A. I., Ramos-Clamont G., Vazquez-Moreno L.

Acta Biochimica Polonica

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2008

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tom 55

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nr 3

491-497

The non-enzymatic reaction between reducing sugars and proteins, known as glycation, has received increased attention from nutritional and medical research. In addition, there is a large interest in obtaining glycoconjugates of pure well-characterized oligosaccharides for biological research. In this study, glycation of bovine serum albumin (BSA) by d-glucose, d-galactose and d-lactose under dry-heat at 60°C for 30, 60, 120, 180 or 240 min was assessed and the glycated products studied in order to establish their biological recognition by lectins. BSA glycation was monitored using gel electrophoresis, determination of available amino groups and lectin binding assays. The BSA molecular mass increase and glycation sites were investigated by mass spectrometry and through digestion with trypsin and chymotrypsin. Depending on time and type of sugar, differences in BSA conjugation were achieved. Modified BSA revealed reduction of amino groups’ availability and slower migration through SDS/PAGE. d-Galactose was more reactive than d-glucose or d-lactose, leading to the coupling of 10, 3 and 1 sugar residues, respectively, after 120 minutes of reaction. BSA lysines (K) were the preferred modified amino acids; both K256 and K420 appeared the most available for conjugation. Only BSA-lactose showed biological recognition by specific lectins.

3

Involvement of envelope-glycoprotein glycans in HIV-1 biology and infection

67%

Raska M., Novak J.

Archivum Immunologiae et Therapiae Experimentalis

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2010

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tom 58

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nr 3

191-208

4

Similarity of vitamin D-binding protein (DBP) between various animal species in double immunodiffusion and western blotting

67%

Madej J. P., Boratynski J., Nowacki W.

Electronic Journal of Polish Agricultural Universities. Series Veterinary Medicine

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2007

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tom 10

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nr 3

5

The fermentation dynamics of sheep milk with increased proportion of whey proteins

67%

Lasik A., Pikul J., Dankow R., Cais-Sokolinska D.

Acta Scientiarum Polonorum. Technologia Alimentaria

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2011

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tom 10

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nr 2

Introduction. A new trend in food production is to use a whey proteins as a food addi- tives. The aim of this research project was to evaluate the impact of increased proportions of whey proteins in sheep milk on the course of its fermentation process. Material and methods. The materials used in the experiments was sheep and cow milk of natural ratio of whey to casein proteins (1:4) and sheep milk in which the ratio of whey to casein proteins amounted to 1:1 and 4:1. The dynamics of the fermentation process and the stability of final products was described on the basis of pH value, titratable acidity, lactose, galactose and lactic acid content. Results. Higher, in relation to natural, ratios of whey to casein proteins (1:1 and 4:1) in sheep milk increased the acidity of the final product after incubation and decreased the degree of fermented lactose. The cooling storage of fermented milks failed to affect changes in pH value, titratable acidity, galactose and lactic acid content of the final products. Conclusion. Changes in ratios of whey to casein proteins result in differences in dynamics of sheep milk fermentation but not influenced on final product's stability.

6

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Dietary fiber extraction from defatted corn hull by hot-compressed water

67%

Wang L., Liu H.-M., Xie A.-J., Zhu C.-Y., Qin G.-Y.

Polish Journal of Food and Nutrition Sciences

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2018

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tom 68

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nr 2

Corn hulls were abundant and inexpensive byproducts of the corn dry or wet milling processes, but most of them were discarded as agro-wastes. The aim of this study was to extract the dietary fiber by hot-compressed water (HCW) from defatted corn hull and to determine the chemical properties. Results showed that temperature and time played critical roles in extraction effi ciency; the maximal yield of dietary fiber A (DFA) extracted by HCW reached 33.0% at 150°C for 60 min. The yield of dietary fiber B (DFB) increased from 2.0% to 56.9% as the temperature increased from 110 to 180°C, while the yield of solid residue (SR) decreased from 88.7% to 27.7%. Fourier transform infrared spectroscopy (FT-IR) results demonstrated that C-H, O-H, C=O, COO- occurred in the DFA, SR and DFB. The dietary fiber polysaccharides consisted of arabinose, galactose, glucose, xylose and uronic acid.

7

Diseases of aberrant glycosylation

67%

Koscielak J.

Acta Biochimica Polonica

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1995

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tom 42

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nr 1

Recently a defective glycosylation of glycoconjugates has been implicated in the pathogenesis of a number of heritable or acquired diseases of humans. Herein I discuss them under the name of diseases of aberrant glycosylation. These are: congenital dyserythropoietic anemia type II, carbohydrate-deficient glycoprotein syndrome, I-cell disease, galactosemia in subjects on galactose-free diet, variants of leukocyte adhesion deficiency, and of Ehlers-Danlos syndrome, paroxysmal nocturnal hemoglobinuria, and Tn syndrome. Regarding the present views on the function of glycoconjugates it is probably significant that in most instances defective or missing glycoproteins (or proteoglycans,) but not glycosphingolipids, are probably involved in the pathogenesis of these diseases.

8

Expression of the yeast NAM9 gene coding for mitochondrial ribosomal protein

67%

Boguta M., Chacinska A., Murawski M., Szczesniak B.

Acta Biochimica Polonica

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1997

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tom 44

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nr 2

We studied expression of the NAM9 gene of Saccharomyces cerevisiae that was previously reported to code for a mitochondrial ribosomal protein. Increase in NAM9 gene dosage is accompanied by the increase in both mRNA and protein. The levels of the NAM9 transcript and protein are both reduced in cells growing on glucose as compared to cells growing on galactose as a carbon source. Nam9p accumulates to the same level in rho(o) and rho(+) cells. These results confirm previous data indicating diverse regulation of different mitochondrial ribosomal protein genes and suggest that expression of Nam9p is not co-ordinated with the expression of other mitochondrial ribosomal components.

9

Serum glycoproteins in patients with hypo- and hyperthyroidism

67%

Kucharz E. J., Olczyk K., Glowacki A., Duraj-Kassem M.

Acta Biochimica Polonica

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1993

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tom 40

|

nr 1

10

Loss of tolerance to desiccation in germinated Norway maple (Acer platanoides L.) seeds. Changes in carbohydrate content

67%

Pukacka S.

Dendrobiology

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2001

|

tom 46

Carbohydrates were analyzed in Norway maple embryo axes and cotyledons after imbibition, in the middle of cold stratification, before germination and during radicle protrusion to 8-10 mm and 20-25 mm. Simultaneously desiccation tolerance of seeds was determined by tetrazolium (TTC) test, after desiccation of seeds to 10-20% of water content. The cotyledons were tolerant to desiccation throughout all stratification and germination period. Embryo axes became sensitive to desiccation when hypocotyls-radicle protrusion reached 20-25 mm length. In this period the significant increase of monosaccharides: glucose, fructose and galactose in embryo axes occurred. This was not observed in cotyledons. During the germination period significant decrease of sucrose and raffinose content was noted in embryo axes and cotyledons. Relatively less changes appeared in stachyose content in embryo axes while in cotyledons it decreased evidently. The mass ratio of sucrose to oligosaccharides was higher in cotyledons of germinated seeds. The marked decrease of mass ratio of oligo to monosaccharides was observed in embryo axes in the last period of germination. The role of carbohydrates in loosing tolerance to desiccation in germinated Norway maple seeds is discussed.

11

Staphylococcal L-asparaginase: Catabolic repression of synthesis

67%

Rozalska M., Mikucki J.

Acta Microbiologica Polonica

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1992

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tom 41

|

nr 3-4

12

Alterations of Tamm-Horsfall protein immunoreactivity after partial desialylation and deglycosylation

67%

Grabska T., Baginski T., Kubicz A., Kokot M., Kokot F., Dulawa J.

Archivum Immunologiae et Therapiae Experimentalis

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1996

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tom 44

|

nr 4

13

A novel tetrameric lectin from Lycoris aurea with four mannose binding sites per monomer

67%

Liu J., Xu X., Liu J., Balzarini J., Luo Y., Kong Y., Li J., Chen F., Van Damme E., Bao J.

Acta Biochimica Polonica

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2007

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tom 54

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nr 1

The mannose-binding agglutinin from bulbs of Lycoris aurea (LAA) agglutinates rabbit but not human erythrocytes. The molecular mass of the monomer in SDS/PAGE is 12 kDa while the apparent molecular mass in gel filtration is 48 kDa, indicating that LAA is a homotetramer. The full-length cDNA of LAA contains 683 bp with an open reading frame encoding a protomer of 162 amino-acid residues. Hydrophobic Cluster Analysis and molecular modeling of the 109-residue mature polypeptide suggested a similar secondary and tertiary structure to those of Narcissus pseudonarcissus agglutinin (NPA). Molecular docking revealed that, besides the three mannose-binding sites common among Amaryllidaceae lectins, LAA also contains a fourth unique mannose-binding site formed by a tryptophan cluster. The existence of four mannose-binding sites in each monomer of LAA is very unusual and has only been reported for NPA earlier.

14

Zaburzenia metabolizmu galaktozy i fruktozy

51%

Wojcik M.

Żywność, Żywienie, Życie. Szkoła Główna Gospodarstwa Wiejskiego w Warszawie

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2002

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nr 1

27-28

15

Production and partial characterization of high molecular weight extracellular alpha-amylase from Thermoactinomyces vulgaris isolated from Egyptian soil

51%

Abou Dobara M., El-Sayed A., El-Fallal A., Omar N.

Polish Journal of Microbiology

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2011

|

tom 60

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nr 1

Optimizing production of α-amylase production by Thermoactinomyces vulgaris isolated from Egyptian soil was studied. The optimum incubation period, temperature and initial pH of medium for organism growth and enzyme yield were around 24 h, 55°C and 7.0, respectively. Maximum α-amylase activity was observed in a medium containing starch as carbon source. The other tested carbohydrates (cellulose, glucose, galactose, xylose, arabinose, lactose and maltose) inhibited the enzyme production. Adding tryptone as a nitrogen source exhibited a maximum activity of α-amylase. Bactopeptone and yeast extract gave also high activity comparing to the other nitrogen sources (NH₄Cl, NH₄NO₃, NaNO₃, KNO₃, CH₃CO2 NH₄). Electrophoresis profile of the produced two α-amylase isozymes indicated that the same pattern at about 135145 kDa under different conditions. The optimum pH and temperature of the enzyme activity were 8.0 and 60°C, respectively and enzyme was stable at 50°C over 6 hours. The enzyme was significantly inhibited by the addition of metal ions (Na⁺, Co²⁺ and Ca²⁺) whereas Cl⁻ seemed to act as activator. The enzyme was not affected by 0.1 mM EDTA while higher concentration (10 mM EDTA) totally inactivated the enzyme.

16

Metabolizm laktozy podczas wyrobu sera edamskiego

51%

Cichosz G., Rymaszewski J., Kujawski M.

Acta Academiae Agriculturae ac Technicae Olstenensis. Technologia Alimentorum

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1995

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tom 27

61-70

17

Acclimation of poplar trees to heavy metals in polluted habitats: I. Carbohydrate metabolism in fine roots of Populus deltoides

51%

Lorenc-Plucinska G., Stobrawa K.

Acta Societatis Botanicorum Poloniae

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2005

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tom 74

|

nr 1

Concentrations of total nonstructural carbohydrates (TNC), soluble carbohydrates, starch, sucrose, glucose, fructose, raffinose, galactose, stachyose, mannitol and specific activities of soluble acid (AI) and neutral (NI) invertases, sucrose synthase (SuSy), hexokinase (HK), fructokinase (FK), glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and glucose 6-phosphate dehydrogenase (G6PDH) were analyzed in fine roots of Populus deltoides Bartr. ex Marsh growing at a polluted site (near copper smelters) and a control site (free from heavy pollution). Also chemical properties of the soil from both sites were assessed. In comparison with the control, fine roots from the polluted site contained greater concentrations of TNC, soluble sugars, starch and sucrose but less hexoses, so they had higher values of sucrolysis index (sucrose/hexoses). The activity of AI, NI and SuSy declined insignificantly, while specific activities of HK, FK, GAPDH and G6PDH were significantly inhibited. The results suggest that a long-term heavy metal stress leads to an accumulation of carbohydrates and altering activities of glycolysis and the oxidative pentose phosphate pathway in fine roots.

18

Rye dietary fibre

51%

Raczynska-Bojanowska K., Rybka K., Madej L., Sitarski J., Osinski R., Rakowska M., Cyran M.

Hodowla Roślin, Aklimatyzacja i Nasiennictwo

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1994

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tom 38

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nr 5

Ograniczanie wyników

Physico-chemical characteristics of different genetic variants of bovine beta-casein, modified covalently by glucose, galactose and lactose

Characterization of bovine serum albumin glycated with glucose, galactose and lactose

Involvement of envelope-glycoprotein glycans in HIV-1 biology and infection

Similarity of vitamin D-binding protein (DBP) between various animal species in double immunodiffusion and western blotting

The fermentation dynamics of sheep milk with increased proportion of whey proteins

Dietary fiber extraction from defatted corn hull by hot-compressed water

Diseases of aberrant glycosylation

Expression of the yeast NAM9 gene coding for mitochondrial ribosomal protein

Serum glycoproteins in patients with hypo- and hyperthyroidism

Loss of tolerance to desiccation in germinated Norway maple (Acer platanoides L.) seeds. Changes in carbohydrate content

Staphylococcal L-asparaginase: Catabolic repression of synthesis

Alterations of Tamm-Horsfall protein immunoreactivity after partial desialylation and deglycosylation

A novel tetrameric lectin from Lycoris aurea with four mannose binding sites per monomer

Zaburzenia metabolizmu galaktozy i fruktozy

Production and partial characterization of high molecular weight extracellular alpha-amylase from Thermoactinomyces vulgaris isolated from Egyptian soil

Metabolizm laktozy podczas wyrobu sera edamskiego

Acclimation of poplar trees to heavy metals in polluted habitats: I. Carbohydrate metabolism in fine roots of Populus deltoides

Rye dietary fibre