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Rapid evolutionary dynamics of the Pepino mosaic virus - status and future perspectives

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Minicka J., Hasiow-Jaroszewska B., Borodynko-Filas N., Pospieszny H., Hanssen I. M.

Journal of Plant Protection Research

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2016

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tom 56

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nr 4

Pepino mosaic virus (PepMV) has emerged as an important pathogen of greenhouse tomato crops and is currently distributed worldwide. Population genetic studies have revealed a shift in the dominant PepMV genotype from European (EU) to Chilean 2 (CH2) in North America and several European countries. New genetic variants are constantly being created by mutation and recombination events. Single nucleotide substitutions in different parts of the genome were found to affect on development of symptoms resulting in new pathotypes and accumulation of viral RNA. The variability of the PepMV population has a great impact on designing specific diagnostic tools and developing efficient and durable strategies of disease control. In this paper we review the current knowledge about the PepMV population, the evolutionary dynamics of this highly infective virus, methods for its detection and plant protection strategies.

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Animal cloning by nuclear transfer: state-of-the-art and future perspectives

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Dinnyes A., Szmolenszky A.

Acta Biochimica Polonica

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2005

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tom 52

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nr 3

Model organisms are essential to study the genetic basis of human diseases. Transgenic mammalian models, especially genetic knock-out mice have catalysed the progress in this area. To continue the advancement, further sophisticated and refined models are crucially needed to study the genetic basis and manifestations of numerous human diseases. Coinciding with the start of the new era of post-genomic research, new tools for establishment of transgenesis, such as nuclear transfer and gene targeting in somatic cells, have become available, offering a unique opportunity for the generation of transgenic animal models. The new technology provides important tools for comparative functional genomics to promote the interpretation and increase the practical value of the data generated in numerous mouse models. This paper discusses the state-of-the-art of the nuclear replacement technology and presents future perspectives.

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Current status and future perspectives of DNA vaccine delivery by attenuated intracellular bacteria

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Dietrich G.

Archivum Immunologiae et Therapiae Experimentalis

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2000

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tom 48

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nr 3

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Biodiversity of freshwater microorganisms - achievements, problems, and perspectives

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Weisse T.

Polish Journal of Ecology

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2006

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tom 54

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nr 4

The extent and significance of the diversity of freshwater microbes is at present controversially debated. Until 1980 it was assumed that there are no freshwater-specific bacteria and that the total number of bacterial species is low. The advent of molecular tools over the last ten years revealed that there is a bacterial freshwater assemblage which is phylogenetically different from soil and marine bacteria; secondly, it became obvious that the total number of cultured bacterial species (~5900) underestimates bacterial diversity by several orders of magnitude. The current debate centres on 1) how to define a bacterial species and 2) if there is a microbial biogeography. The latter relates to the issue of ubiquity and cosmopolitanism, which is controversially discussed primarily in relation to eukaryotic microorganisms, namely ciliates. Although solid evidence is scarce, many microbial ecologists assume, in accordance with Baas Becking’s famous 70-year old dictum – “everything is everywhere, the environment selects” – that freshwater microorganisms are easily dispersed and, therefore, potentially cosmopolitan. This review focuses on the often neglected second part of Baas Becking’s metaphor. Evidence is accumulating rapidly that the environment does not simply act as a filter sensu Gleason’s individualistic concept for widely dispersed microbes. Rather, prokaryotic and eukaryotic microorganisms have adapted to their specific habitat and perform better in this environment than newly invading congeners. There is an enormous ecophysiological diversity among closely related freshwater microbes which is neither obvious at the morphospecies level nor at the level of evolutionarily conserved genes, such as the small ribosomal RNA gene. Although this large diversity has been demonstrated for various groups of bacteria and protists, there is currently no measure available to compare microbial biodiversity across prokaryotic and eukaryotic domains. The current challenge is to link genetic divergence to ecophysiological diversity in the major taxa.

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A review of the in vitro propagation of Bauhinia spp.

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Teixeira da Silva J. A.

Journal of Horticultural Research

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2013

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tom 21

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nr 1

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Synthesis of aminophospholipids in Saccharomyces cerevisiae and Chinese hamster ovary cells. From mutagenesis to genes and cellular function

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Lenart J., Pikula S.

Cellular and Molecular Biology Letters

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1998

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tom 03

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nr 2

The purpose of this article is to provide a concise overview of the characterization of auxotrophic mutated cells to the precursors of lipid synthesis, and of the identification of specific genes encoding enzymatic proteins involved in this process. The focus is on enzymes catalyzing the synthesis of phosphatidylserine and phosphatidylethanolamine in Saccharomyces cerevisiae and Chinese hamster ovary cells, two cell types frequently used by investigators studying the mechanisms of genetic control of metabolic processes.

Ograniczanie wyników

1 Acta Biochimica Polonica

1 Archivum Immunologiae et Therapiae Experimentalis

1 Cellular and Molecular Biology Letters

1 Journal of Horticultural Research

1 Journal of Plant Protection Research

1 Polish Journal of Ecology

1 Borodynko-Filas N.

1 Dietrich G.

1 Dinnyes A.

1 Hanssen I. M.

1 Hasiow-Jaroszewska B.

1 Lenart J.

1 Minicka J.

1 Pikula S.

1 Pospieszny H.

1 Szmolenszky A.

1 Teixeira da Silva J. A.

1 Weisse T.

1 2016

1 2013

1 2006

1 2005