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Many ecological terms are very difficult to define, or to apply to some groups of organisms. In the case of fungi, it is unclear how to speak of individuals, populations and species. Relying on morphological characters often gives ambiguous results. However, in recent years new techniques have been developed, techniques that provide us with robust tools, helpful in recognizing specific groups in nature. This paper aims to give a short overview of how molecular methods are used to delimitate species, individuals and populations in fungi.
A biotic interaction between fungi from soil within and outside the rhizosphere of potato and fungi responsible for black scurf – Rhizoctonia solani Kühn and silver scurf – Helminthosporium solani (Dur., Mont.). It was found that fungi population connected with crop environment under investigation promoted the growth of Rhizoctonia solani, thus indicating no resistance of this environment to this pathogen. These fungi, however, inhibited the growth of Helminthosporium solani.
The aim of this work was 1) to investigate the genetic diversity of H. annosum s. str. populations, 2) to recognize genets ability of their aggressiveness to Scots pine. The genetic similarity among genets varied from 0% to 74%. Totally different genets were found in 17.9% of cases. The lowest similarity occurred among 20.7% of genets and it was calculated on 18%. Six months after inoculations of Scots pine stems by H. annosum s. str. the distance of wood colonization by mycelium was measured. Wood was colonized to a distance varying from 0.52 cm to 2.06 cm. The most aggressive genets were isolated from stumps, and the less aggressive from trees' stems. The infections in stands caused by H. annosum s. str. basidiospores and appearance of a new pathogen's organisms, which might distinguish by high aggressiveness, could increase a damages in these stands. There is still a need to use a biological control against root rot disease to reduce spreading of a new generations of pathogens.
The purpose of the studies conducted in the years 1996-1998 was to determine the quantitative composition of bacteria and fungi populations in the rhizosphere of spring wheat, winter wheat, potato and soybean, and in non-rhizosphere soil. Besides, the effect of root exudates of these plants on the formation of antagonistic microorganisms is presented. A microbiological analysis found out that 1 g of rhizosphere soil dry weight of the examined plants and non-rhizosphere soil contained from 4.24 x 10⁶ to 5.97 x 10⁶ bacteria colonies on average. The lowest number of bacteria was found in non-rhizosphere soil (4.24 x 10⁶ on average), and the highest in rhizosphere of potato (5.97 x 10⁶ on average). The fewest fungi colonies (28.59 x 10³ on average) were isolated from 1 g of dry weight of winter wheat rhizosphere, and the most (93.41 x 10³) from soybean rhizosphere. Antagonistic bacteria of genera Bacillus and Pseudomonas, and fungi of Gliocladium, Penicillium and Trichoderma genera dominated in winter wheat rhizosphere. Soybean roots exuded the greatest number of aminoacids (1.088 mg/ml of the solution), while spring wheat roots exuded the smallest amount (0.148 mg/ml of the solution). The percentage of aromatic and alkaline aminoacids was the lowest in potato root exudates, whilc the highest was found out in the exudates of winter wheat.
The formation of phenotypic structure of P. infestans population in Poland was determined by analyzing 1603 isolates collected from 1987 to 2001. The race complexity, low at the beginning of experiment, has been increasing from year to year and reached in 2001 a high level 7.2 virulence factors per isolate. The single and less composed races dominating first1y in the population were replaced gradually by more composed races. The virulence factors 1, 2, 3, 4, 7 and 11 occurred most frequent1y, but factors 5 and 8 were noted sporadically. The A2 mating type was detected in 1988 at first and since that time its occurrence has been noted in Polish population each year. The oospores were formed in potato tissues. Race diversity, low at the beginning of the investigation, reached a peak in 1996-2001. During 1987-1990 weakly and middly aggressive phenotypes dominated in Polish population. In the later period very aggressive isolates were more frequent. Phenotypic race similarity of P. infestans populations in 1987 and 2001 was very low. The race structure of 1987 population was totally different from the race structure of populations of the next years. it was probably due to migration of new pathotypes. On the other hand the variation in complexity, diversity and similarity of races, as well as in aggressiveness observed in later years of investigation can be caused by the presence of both mating types and sexual recombination.
The presence of pathogenic fungi at each stage of plant development may cause a reduction in grain quality and significant losses in yield. The aim of the presented study was to compare the population of the most commonly pathogens causing diseases of stem base of wheat in two important wheat-growing regions: in the Wielkopolska Province and the Warmia-Mazury Province. The material designed for investigating was collected in 2007–2008 and came from randomly chosen locations, most of which were individual farmlands. In order to identify pathogens, fragments of plants were taken (stem base) with symptoms of infestation by pathogenic fungi. Among the isolates obtained 66% constituted fungi of the genus Fusarium. But dominant pathogen in Wielkopolska was Rhizoctonia cerealis. Differences were found in populations of fungi on stem base of wheat in the explored areas.
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