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  1. 20 Progress in Plant Protection

  2. 7 Zeszyty Problemowe Postępów Nauk Rolniczych

  3. 5 Acta Agrobotanica

  4. 2 Acta Mycologica

  5. 2 Journal of Plant Protection Research

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  1. 4 Pieczul K.

  2. 4 Piwoni A.

  3. 4 Trzewik A.

  4. 3 Jedryczka M.

  5. 3 Korbas M.

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  1. 1 2018

  2. 1 2017

  3. 1 2016

  4. 14 2010

  5. 2 2009

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1
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Morphological characterization of biocontrol isolates of Trichoderma to study the correlation between morphological characters and biocontrol efficacy

100%
Kumar M., Sharma P.
International Letters of Natural Sciences
|
2016
|
tom 55
The morphological characterization was carried out for 5 isolates of Trichoderma harzianum and 7 isolates of Trichoderma viride and tested for their biocontrol efficacy. The isolates belonging to T.harzianum were analogous in colony colour, culture smell, mycelial colour, conidiation, conidial shape, conidial wall and conidial colour. Correspondingly the isolates of T.viride showed certain similarity in colony colour, colony edge, culture smell, conidiophore branching, conidial wall, conidial colour and chlamydospores. Inter specific differences through cluster analysis based on morphological characters grouped the twelve isolates into three major clusters where all the isolates of T.harzianum formed a single cluster while the isolates of T.viride were bifurcated into two groups. The clustering was substantiated by similarity index which showed maximum similarity among T.harzianum isolates with only less than 20% variation among themselves. Similarly the clusters having isolates of T.viride also had less variation within them. The biocontrol efficacy of these twelve isolates of Trichoderma was experimented by dual culture test under laboratory condition and there existed some relation between the biocontrol efficacy of these isolates and morphology.
2

Compatibility of Metarhizium anisopliae [Ascomycota: Hypocreales] with several insecticides

84%
Rashid M., Baghdadi A., Sheikhi A., Pourian H.-R., Gazavi M.
Journal of Plant Protection Research
|
2010
|
tom 50
|
nr 1
22-27
The entomopathogenic fungus conidia viability and sporulation may be affected by different environmental factors or by bio-pesticides and chemical products used to protect cultivated plants. In this research, we investigated the compatibility of Metarhizium anisopliae DEMI 001 isolates with three insecticides: fipronil, pyriproxyfen and hexaflumuron. The effect of fipronil, pyriproxyfen and hexaflumuron on sporulation, vegetative growth and conidial germination of the fungus was studied based on measuring the vegetative growth and sporulation. SDA medium was mixed with fipronil (10, 40 and 120 ppm), pyriproxyfen (10, 500 and 1 000 ppm), hexaflumuron (20, 50 and 120 ppm). SAD medium excluding insecticides was designated as blank. To determine the sporulation rate, spores was counted on surface area and vegetative growth was measured on the basis of colony diameter. The results showed that hexaflumuron at the concentration of 120 ppm reduced the vegetative growth to 0% showing the highest reduction effect compared to pyriproxyfen (24.59%) and fipronil (24.31%). All three insecticides reduced drastically the conidial germination at the highest concentrations (0-15%). Hexaflumuron treatment with 0% germination at all three concentrations and fipronil and pyriproxyfen with 32.36% and 9.7% germination at 200 ppm were not significantly different. Regarding the highest negative effect of hexaflumuron on germination at all three concentrations, this insecticide should not be applied together with the entomopathogenic fungus.
3

Production and characterization of alpha-amylase from Aspergillus niger JGI 24 isolated in Bangalore

84%
Varalakshmi K. N., Kumudini B. S., Nandini B. N., Solomon J., Suhas R., Mahesh B., Kavitha A. P.
Polish Journal of Microbiology
|
2009
|
tom 58
|
nr 1
29-36
Five fungal isolates were screened for the production of α-amylase using both solid-state and submerged fermentations. The best amylase producer among them, Aspergillus niger JGI 24, was selected for enzyme production by solid-state fermentation (SSF) on wheat bran. Different carbon and nitrogen supplements were used to enhance enzyme production and maximum amount of enzyme was obtained when SSF was carried out with soluble starch and beef extract (1 % each) as supplements. Further attempts to enhance enzyme production by UV induced mutagenesis were carried out. Survival rate decreased with increase in duration of UV exposure. Partial purification of the enzyme using ammonium sulphate fractionation resulted in 1.49 fold increase in the enzyme activity. The enzyme showed a molecular weight of 43 kDa by SDS-PAGE. Metal ions Ca²⁺ and Co²⁺ increased the enzyme activity. The enzyme was optimally active at 30°C and pH 9.5.
4

Effects of foliar fungicides on the mycoflora of glumes of Triticum aestivum

84%
Blaszkowski J.
Acta Mycologica
|
1995
|
tom 30
|
nr 1
5
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Development of thermotolerant isolates of Beauveria bassiana (Bals.-Criv.) Vuill. with ethyl methanesulfonate

67%
Wongwanich Y., Cobelli P., Boonchuay D., Wangsomboondee T.
Journal of Plant Protection Research
|
2017
|
tom 57
|
nr 4
Beauveria bassiana is an entomopathogenic fungus that is widely used in Thailand to control pest insects. However, the increasing temperature has influenced the insect control efficiency of the fungus. Therefore, determination of thermotolerant isolates of B. bassiana that can grow and remain pathogenic at higher temperatures than its current optimum temperature may be a better way to control pest insects in a high temperature environment. Three isolates of B. bassiana obtained from the Rice Department, Thailand were selected for mutagenesis using ethyl methanesulfonate (EMS) with subsequent screening at high temperatures (33 and 35°C). In addition, the recovery of fungal growth after exposure to a high temperature for a period of time (5–15 days) and then transferring to 25°C was evaluated. No isolates were found that grew at 35°C but one mutant isolate (BCNT002MT) produced larger diameter colonies and more spores than the corresponding wild type (WT) at 33°C. Growth and spore production of the BCNT002MT isolate were greater than its WT when incubated at 25°C for 14 days following exposure to 33°C for 7 days. In addition, the spore germination level (%) of BCNT002MT was significantly higher than its WT during culture at 25°C after prior exposure to 33°C for 5, 10 and 15 days. The pathogenicity against the brown planthopper, Nilaparvata lugens (Stål), of this mutant isolate was also prominent.
6

Begonia semperflorens – nowy żywiciel dla Phytophthora cactorum w Polsce

67%
Orlikowski L. B., Ptaszek M.
Progress in Plant Protection
|
2010
|
tom 50
|
nr 2
In 2009 on Begonia semperflorens stem base, brown conical spots spreading upwards to the first branching were observed. Affected plants wilted slowly and died. From diseased tissues Phytophthora cactorum was mainly isolated. Botrytis cinerea, Fusarium avenaceum and F. culmorum occurred only sporadically. In laboratory trials P. cactorum colonized leaves of Begonia semperflorens and Begonia × hybrida with significantly faster development of necroses on the first species. Among 4 cultivars of begonia significantly faster colonization of leaf petioles was observed on Willem Kusters. In greenhouse trial the pathogen colonized leaves and stems of B. sempervirens.
7

Patogenicznosc izolatow Fusarium avenaceum [Fr.] Sacc. w stosunku do cebul i korzeni tulipana

67%
Piwoni A.
Progress in Plant Protection
|
2003
|
tom 43
|
nr 2
872-875
8

Patogeniczność izolatów Botrytis narcissicola Kleb. w stosunku do 5 odmian narcyza w warunkach pędzenia

67%
Piwoni A.
Progress in Plant Protection
|
2009
|
tom 49
|
nr 1
9

Patogenicznosc izolatow Botrytis narcissicola Kleb. w stosunku do narcyza odmiany Carlton w warunkach pedzenia

67%
Piwoni A.
Progress in Plant Protection
|
2008
|
tom 48
|
nr 2
504-507
10

Utajony wirus oliwki 1 (Olive latent virus 1) z pomidora

67%
Pospieszny H., Borodynko N., Hasiow-Jaroszewska B.
Zeszyty Problemowe Postępów Nauk Rolniczych
|
2010
|
tom 554
W roku 2008, z liści pomidora z objawami nekroz, rosnącego w szklarni w województwie wielkopolskim, wyizolowano wirusa, którego oznaczono jako CM1. Wirus posiada cząstki sferyczne o średnicy około 30 nm i genom w postaci jednoniciowego RNA, o wielkości 3700 nukleotydów. Wirus przeniesiony mechanicznie na rośliny testowe porażał szeroki zakres różnych gatunków roślin, na których powodował objawy lokalne z wyjątkiem Nicotiana benthamiana i N occidentalis, które porażał także systemicznie. RT-PCR ze starterami wiążącymi się niespecyficznie do matrycy (ang. random hexamer primers) oraz sekwencjonowanie uzyskanych produktów pozwoliły na wstępną identyfikację utajonego wirusa oliwki 1 (Olive latent virus 1, OLV-1). Na podstawie sekwencji OLV-1 dostępnych w Banku Genów zaprojektowano startery amplifikujące gen kodujący białko płaszcza (CP) wirusa. Porównanie uzyskanych sekwencji CP potwierdziło identyfikację i wykazało istotne różnice pomiędzy izolatami z pomidora a pozostałymi, i tak: podobieństwo z sekwencją izolatu OLV-1 z tulipana wynosiło 92,5%, z izolatem z oliwki 91,8% i z drzewa cytrusowego 89,5%. Jest to zarówno pierwsza identyfikacja OLV-1 w Polsce, jak i stwierdzenie nowego jego gospodarza jakim jest pomidor.
11

Charakterystyka polskich izolatow grzyba Sclerotinia sclerotiorum z porazonych roslin rzepaku ozimego

67%
Lewartowska E., Jedryczka M., Frencel I.
Progress in Plant Protection
|
1996
|
tom 36
|
nr 2
179-181
12

Wpływ olejku herbacianego na wzrost grzybni kilku krajowych izolatów grzybów rodzaju Trichoderma w warunkach in vitro

67%
Siwulski M., Sobieralski K., Gorski R.
Progress in Plant Protection
|
2010
|
tom 50
|
nr 4
The studies were conducted to determine the effect of tea tree oil on in vitro mycelium growth of some Trichoderma isolates. Seven isolates of T. harzianum (Th1) and seven isolates of T. atroviride (Th3) were tested. The growth of Trichoderma isolates was studied on PDA media with tea tree oil at the concentration of: 0.05; 0.1; 0.2 and 0.3%. The studies proved that the applied tea tree oil was effective in mycelium growth inhibition of T. harzianum and T. atroviride at the concentration of 0.2 and 0.3%.
13

Metody stosowane do charakteryzowania izolatów Phytophthora infestans, organizmu powodującego zarazę ziemniaka

67%
Sliwka J.
Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin
|
2002
|
tom 223-224
337-349
Praca jest przeglądem literatury dotyczącej rodzajów polimorfizmu występujących wśród izolatów Phytophthora infestans, organizmu powodującego ważną chorobę ziemniaka — zarazę ziemniaka. Skupiono się głównie na metodach wykrywania poszczególnych rodzajów polimorfizmu. Szczegółowo omówione zostały metody określania: typu kojarzeniowego, odporności na metalaksyl, wirulencji, agresywności, patogeniczności, izoenzymów peptydazy i izomerazy glukozo-6-fosforanu, polimorfizmu długości fragmentów restrykcyjnych DNA jądrowego (sonda RG-57) oraz haplotypu mitochondrialnego. Wspomniano o testach opartych na metodzie PCR pozwalających wykrywać typ kojarzeniowy i polimorfizm w obrębie znanych sekwencji P. infestans, a także o markerach RAPD.
14

Ocena zmienności morfologicznej wybranych izolatów Fusarium culmorum [W.G. Smith] Sacc., Fusarium nivale [Fr.] Ces [Microdochium nivale Samuels et Halett] i Fusarium avenaceum [Corda ex Fr.] Sacc

67%
Koczowska I., Packa D., Wiwart M.
Acta Academiae Agriculturae ac Technicae Olstenensis. Agricultura
|
1993
|
tom 56
31-52
Badano zmienność dynamiki wzrostu i sporulacji 12 izolatów należących do gatunków Fusarium culmorum, F. nivale (Microdochium nivale) i F. avenaceum. Wzrost grzybni przebiegał na trzech pożywkach (PDA, SNA i Czapek-Doxa). Zaobserwowano znaczne różnice w intensywności zarodnikowania, natomiast zróżnicowanie dynamiki wzrostu izolatów było niewielkie. Pożywką najlepiej różnicującą tę cechę okazała się SNA.
15

Physiological and biochemical changes induced by osmolarity in halotolerant Aspergilli

67%
Mahdy H. M., El-Sheikh H. H., Ahmed M. S., Refaat B. M.
Acta Microbiologica Polonica
|
1996
|
tom 45
|
nr 1
16
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Use of random amplified polymorphic DNA [RAPD] assay for differentiation among isolates of Stagonospora spp. and Septoria tritici

67%
Czembor P. C., Arseniuk E.
Journal of Applied Genetics
|
1996
|
tom 37
|
nr 3
The genetic similarity of three species: Septoria tritici, Stagonospora nodorum and Stagonospora avenae f. sp. triticea - important pathogens in many cereal production areas worldwide was assessed by random amplified polymorphic DNA (RAPD) assay. In preliminary research DNA of 14, 9, and 7 monopyenidios- pore isolates of S. nodorum, S. tritici, and S. a. tritícea, respectively, were amplified by PCR with four primers. Afterwards the research was focused on three mono- pyenidiospore isolates from each species studied. The isolates of each species selected for the study varied in pathogenicity and were diverse geographically. PCR with the set of 14 selected primers resulted in 99 different bands, ranged from 180 to 2500 base pairs in length. Most primers in PCR (especially RAD11, RAD31, RAD32, RAD33) revealed uniform bands for isolates of S. a. tritícea, that allow to identify this species among the others. The cluster analysis using Unweighed Pair-Group Method with Averaging (UPGMA) revealed interspecies disagreement among the isolates ranging from 32 to 53%. The intraspecies disagreement ranges were 17-20%, 38-43%, 42-44% for S. avenae f. sp. triticea, S. nodorum and S. tritici, respectively. Cluster analysis classified isolates into three homogeneous clusters. Each cluster grouped isolates of one species according to their current taxonomie ranks based on spore size, colony morphology and host ranges. In addition, two of the clusters represented by isolates of S. nodorum and S. a. tritícea were distinctly separated at a lower linkage distance from the third one comprising isolates of S. tritici. A slight inconsistency found in grouping some isolates indicates that such groupings should be done with caution. The present study indicates that the PCR- RAPD assay is of a potential use in taxonomy of Stagonospora spp. and Septoria tritici as well as in molecular identification of casual disease agents.
17

Patogeniczne grzyby szparaga [Asparagus officinalis L.] z regionu Wielkopolski w 2007 roku

59%
Stepniewska-Jarosz S., Pukacka A.
Progress in Plant Protection
|
2008
|
tom 48
|
nr 2
541-543
18

Ksztaltowanie sie fenotypowej struktury populacji Phytophthora infestans w Polsce w ciagu 15-lecia 1987-2001

59%
Zarzycka H., Sobkowiak S., Lebecka R., Tatarowska B.
Acta Agrobotanica
|
2002
|
tom 55
|
nr 1
389-400
The formation of phenotypic structure of P. infestans population in Poland was determined by analyzing 1603 isolates collected from 1987 to 2001. The race complexity, low at the beginning of experiment, has been increasing from year to year and reached in 2001 a high level 7.2 virulence factors per isolate. The single and less composed races dominating first1y in the population were replaced gradually by more composed races. The virulence factors 1, 2, 3, 4, 7 and 11 occurred most frequent1y, but factors 5 and 8 were noted sporadically. The A2 mating type was detected in 1988 at first and since that time its occurrence has been noted in Polish population each year. The oospores were formed in potato tissues. Race diversity, low at the beginning of the investigation, reached a peak in 1996-2001. During 1987-1990 weakly and middly aggressive phenotypes dominated in Polish population. In the later period very aggressive isolates were more frequent. Phenotypic race similarity of P. infestans populations in 1987 and 2001 was very low. The race structure of 1987 population was totally different from the race structure of populations of the next years. it was probably due to migration of new pathotypes. On the other hand the variation in complexity, diversity and similarity of races, as well as in aggressiveness observed in later years of investigation can be caused by the presence of both mating types and sexual recombination.
19

Sposob utrzymywania wysokiej agresywnosci izolatu Fusarium sulphureum w stosunku do ziemniaka

59%
Sobkowiak S.
Progress in Plant Protection
|
2005
|
tom 45
|
nr 2
1087-1090
20

Ocena patogenicznosci odmiennych genetycznie izolatow Fusarium culmorum pochodzacych z podstawy zdzbla pszenicy ozimej

59%
Filoda G., Pieczul K., Korbas M.
Progress in Plant Protection
|
2008
|
tom 48
|
nr 1
168-171
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