Wyniki wyszukiwania

1

Functional eicosanoid test and typing [FET] of peripheral blood cells in eicosanoid related diseases

100%

Schafer D., Baenkler H. W.

Journal of Physiology and Pharmacology. Supplement

|

2005

|

tom 56

|

nr 5

103-118

Monitoring of eicosanoid synthesis in peripheral blood cells has significant potential for improving the diagnosis and therapy of many human diseases. The quantitative relation between concentrations of prostaglandins and leukotrienes is central to the physiologic function of the eicosanoid network. Here we show that this regulation, which we call the functional eicosanoid typing (FET), fluctuates dynamically in individual living blood cells from patients, thereby limiting the accuracy with which concentration circuits of eicosanoids can transfer metabolic information. Using living cells in functional cell testing, we characterised the eicosanoid pattern score (EPS). A novel technique based on binomial errors on lipid mediator partitioning enabled calibration of in vivo biochemical parameters in molecular units. We found that eicosanoid production rates fluctuate over a time scale of about twenty minutes, while intrinsic noise decays rapidly. Thus, biochemical eicosanoid parameters, noise, and slowly varying cellular states together determine the effective FET. These results can form a basis for quantitative modelling of natural eicosanoid circuits in diagnosis of eicosanoid related diseases and design of synthetic ones for the prediction other diseases.

2

Functional-eicosanoid-test (FET) and disease

80%

Baenkler H.-W.

Journal of Physiology and Pharmacology. Supplement

|

2006

|

tom 57

|

nr 12

65-72

Eicosanoids are involved in most cellular activities. Measurement of their levels in tissue or blood renders information about the function of activated cells. An extended analysis will improve the conclusions regarding eicosanoid-related diseases. Peripheral white blood cells (WBC) were used for the test. Stimulating or inhibiting substances to influence the generation and the metabolism of eicosanoids were separately added to the samples. Prostaglandins (PG) and leukotrienes (LT) were measured after incubation in culture medium for 20 minutes at room temperature. Healthy controls rendered normal data. Patients with intolerance to acetylsalicylic acid (ASS) showed an elevated output of PG and LT upon stimulation. Addition of ASS shifted from PG to LT. An altered pattern of eicosanoids also was found in patients suffering from gastroduodenal ulcer and in intestinal malignancy. The senstivity regarding the ASS-intolerance is >80% and the specifity in the same group >70%. We concluded that the FET is a suitable test for the demonstration and verification of intolerance to ASS. It also detects an imbalance of the eicosanoids in intestinal malignancy. This makes the FET a helpful tool for diagnosis and for the elucidation of pathogenic mechanisms.

3

Functional eicosanoid test and typing [FET] in acetylsalicylic acid intolerant patients with urticaria

80%

Velten F. W., Bayerl C., Baenkler H.-W., Schaefer D.

Journal of Physiology and Pharmacology. Supplement

|

2006

|

tom 57

|

nr 12

35-46

One of the common side effects of acetylsalicylic acid (ASA) is the induction of pseudoallergic reactions that range from urticarial wheals to anaphylactic shock. At present there is no reliable detection method available for the diagnosis of ASA-hypersensitivity and its relation to clinical symptoms. The purpose of the present study was to evaluate the functional eicosanoid typing (FET) score taking into account several parameters of the equilibrium between prostaglandins (PG) and peptido-leukotriens (pLT). A total eicosanoid pattern score (TEP) ranging from 0.0 to 3.0, was defined that exhibited significant differences (p 0.001) between ASA-intolerant patients and healthy subjects. In addition to the differentiation of both groups at a TEP cut-off value of 1.0, the increasing TEP values correlated with an increasing severity of clinical symptoms in ASA-intolerant patients. We conclude that the FET has the potential for the safe and reliable detection of ASA-intolerance and, probably, other eicosanoid-related pseudoallergic reactions.

Ograniczanie wyników

3 Journal of Physiology and Pharmacology. Supplement

2 Baenkler H.-W.

1 Baenkler H. W.

1 Bayerl C.

1 Schaefer D.

1 Schafer D.

1 Velten F. W.

2 2006

1 2005

Functional eicosanoid test and typing [FET] of peripheral blood cells in eicosanoid related diseases

Functional-eicosanoid-test (FET) and disease

Functional eicosanoid test and typing [FET] in acetylsalicylic acid intolerant patients with urticaria