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Functional and biochemical characterization of Arabidopsis calcium sensor SCS a potential regulator of SnRK2 protein kinases

100%

Bucholc M., Goch G., Ciesielski A., Anielska-Mazur A., Dobrowolska G.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2013

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tom 94

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nr 2

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Functional characterization of begging calls in Thin-billed Prions Pachyptila belcheri chicks

100%

Duckworth A., Masello J. F., Mundry R., Quillfeldt P.

Acta Ornithologica

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2009

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tom 44

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nr 2

Chicks beg to solicit food and care from their parents. Previous studies of nocturnally feeding Thin-billed Prions (Pachyptila belcheri) showed that chicks convey information about their condition to their parents by means of begging calls. However, those studies used tape-recorders with a limited recording duration, which precluded the recording of complete nights, so only the first begging sessions per night were analysed. Here we present data on begging call intensities and the acoustic parameters of begging elements obtained using digital voice recorders, which enabled complete nights to be recorded. Chicks used from one to five begging sessions per night. We found that the parameters of the first calling sessions did not reflect body condition, whereas the duration of begging sessions and the number of begging calls over the whole night was correlated with the chicks' body condition and the sizes of the meals delivered by the parents. The acoustic parameters of begging call elements were not correlated with body condition. Chicks did not change call frequencies according to their state of nutrition. All call parameters, including the acoustic parameters of chick begging calls, were highly chick-specific. We further tested for age effects and found strong correlations between call features and the age of chicks. The results of the present study show that some begging parameters, e.g. the duration of begging sessions and the number of begging calls over the whole night, are connected with condition, while others, such as acoustic parameters, are linked with individual chick recognition.

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Functional characterization of A. thaliana chloroplastic Prx II E

100%

Treffon P., Konig J., Dietz K.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2013

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tom 94

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nr 2

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Functional characterization of NRT1.2-like proteins in cucumber

100%

Warzybok A., Migocka M., Reda M., Klobus G.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2013

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tom 94

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nr 3

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Functional and transcriptomic characterization of a dye-decolorizing fungus from Taxus rhizosphere

100%

Hao D. C., Song S. M., Cheng Y., Qin Z. Q., Ge G. B., An B. L., Xiao P. G.

Polish Journal of Microbiology

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2018

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tom 67

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nr 4

We isolated three laccase-producing fungus strains from Taxus rhizosphere. Myrotheium verrucaria strain DJTU-sh7 had the highest laccase activity of 216.2 U/ml, which was increased to above 300 U/ml after optimization. DJTU-sh7 had the best decolorizing effect for three classes of reactive dyes. The DJTU-sh7-containing fungal consortium displayed the robust decolorizing ability. Both color removal efficiency and chemical oxygen demand were increased in the consortium mediated biotransformation. Transcriptome changes of M. verrucaria elicited by azo dye and phenolic were quantified by the high throughput transcriptome sequencing, and the activities of the selected oxidases and reductases were determined. The possible involvement of oxidases and reductases, especially laccase, aryl alcohol oxidase, and ferric reductase in the biotransformation of dye and phenolic compounds was revealed at both transcriptomic and phenotypic levels. Revealing the transcriptomic mechanisms of fungi in dealing with organic pollutants facilitates the fine-tuned manipulation of strains in developing novel bioremediation and biodegradation strategies.

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Expression analysis and functional characterization of a cold-responsive gene COR15A from Arabidopsis thaliana

100%

Liu D., Li W., Cheng J., Hou L.

Acta Physiologiae Plantarum

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2014

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tom 36

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nr 09

The cold-responsive (COR) genes play an important role in cold acclimation of higher plants. Here, a tight correlation between chloroplast functionality and COR15A expression, and the functional characterization of Arabidopsis COR15A involved in salt/osmotic stress, were revealed. COR15A gene is light inducible and expressed in light-grown seedlings. The expression level of COR15A was reduced when chloroplasts were damaged by norflurazon treatment. By using several albino mutants, seca1, secy1, and tic20, all of which exhibited severe defects in both structure and function of chloroplast, it was shown that the accumulation of COR15A mRNA depends on chloroplast functionality. Real-time RT-PCR and GUS-staining assays demonstrated that COR15A was induced by salt/osmotic stress partially via ABA. Overexpression of COR15A in Arabidopsis resulted in the seedlings displaying hypersensitivity to salt/osmotic stress. All these results suggest that plant acquire the ability to fully express COR15A only after the development of functional chloroplasts, COR15A may be involved in response to salt/ osmotic stress during early stages of plant development.

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Data structures in models of informatively-administrative architecture of coal-mining enterprises

100%

Shevchenko S.

Teka Komisji Motoryzacji i Energetyki Rolnictwa

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2013

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tom 13

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nr 3

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Establishing and functional characterization of an HEK-293 cell line expressing autofluorescently tagged beta-actin [pEYFP-actin] and the neurokinin type 1 receptor [NK1-R]

100%

Hrovat A., Zavec A. B., Pogacnik A., Frangez R., Vrecl M.

Cellular and Molecular Biology Letters

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2010

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tom 15

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nr 1

55-69

This study focused on establishing and making a comprehensive functional characterization of an HEK-293-transfected cell line that would coexpress the enhanced yellow fluorescent protein-actin (pEYFP-actin) construct and the neurokinin type 1 receptor (NK1-R), which is a member of the seven transmembrane (7TM) receptor family. In the initial selection procedure, the cloning ring technique was used alone, but failed to yield clones with homogenous pEYFP-actin expression. Flow cytometry sorting (FCS) was subsequently used to enrich the pEYFP-actin-expressing subpopulation of cells. The enzyme-linked immunosorbent assay (ELISA), FCS and quantitative real-time reverse transcription/polymerase chain reaction (RT-PCR) were then employed to monitor the passage-dependent effects on transgene expression and to estimate the total β-actin/pEYFP-actin ratio. NK1-R was characterized via radioactive ligand binding and the second messenger assay. The suitability of the pEYFP-actin as a marker of endogenous actin was assessed by colocalizing pEYFP-actin with rhodamine-phalloidine-stained F-actin and by comparing receptor- and jasplakinolide-induced changes in the actin cytoskeleton organization. These experiments demonstrated that: i) both constructs expressed in the generated transfected cell line are functional; ii) the estimated pEYFP-actin: endogenous β-actin ratio is within the limits required for the functional integrity of the actin filaments; and iii) pEYFP-actin and rhodamine-phalloidine-stained F-actin structures colocalize and display comparable reorganization patterns in pharmacologically challenged cells.

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Functional characterisation of tissue specific acclimation to oxidative stress

80%

Spruyt L., Hoeberichts F., Muhlenbock P., Van Breusegem F.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2013

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tom 94

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nr 2

Ograniczanie wyników

Functional and biochemical characterization of Arabidopsis calcium sensor SCS a potential regulator of SnRK2 protein kinases

Functional characterization of begging calls in Thin-billed Prions Pachyptila belcheri chicks

Functional characterization of A. thaliana chloroplastic Prx II E

Functional characterization of NRT1.2-like proteins in cucumber

Functional and transcriptomic characterization of a dye-decolorizing fungus from Taxus rhizosphere

Expression analysis and functional characterization of a cold-responsive gene COR15A from Arabidopsis thaliana

Data structures in models of informatively-administrative architecture of coal-mining enterprises

Establishing and functional characterization of an HEK-293 cell line expressing autofluorescently tagged beta-actin [pEYFP-actin] and the neurokinin type 1 receptor [NK1-R]

Functional characterisation of tissue specific acclimation to oxidative stress