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Fumigant toxicity of the essential oil derived from Carum copticum C. B. Clarke (Apiaceae) were assessed against growth stages of Indian meal moth, Plodia interpunctella (Hubner) (Lepidoptera: Pyralidae). Seeds of the plant were collected from Tehran, Iran and hydrodistilled to extract their essential oil. The essential oil was analyzed by GC and GC-MS. Thymol (64.51%), γ-terpinene (17.52%) and p-cymene (16.16%) were the main components among the eight constituents characterized in the oil, representing 98.19% of the total components detected. All bioassay tests were conducted at 25 ± 1°C, 65±5% RH and photoperiod of 16:8 h (light:dark). After preliminary dose-setting experiments, LC50 and LC90 values of four growth stages including eggs, larvae, pupae and adults were calculated. Results showed that the toxicity on the growth stages of Indian meal moth was differed markedly. Adult insects were about 500 times (LC50 = 257.83 μl/m3 air and LC90 = 598.94 μl/m3 air) more susceptible than other growth stages. Moreover, last instar larvae (LC50 = 91.36 μl/l air and LC90 = 213.79 μl/l air) and pupae (LC50 = 105.69 μl/l air and LC90 = 203.24 μl/l air) were significantly more susceptible than eggs (LC50 = 184.61 μl/l air and LC90 = 435.32 μl/l air). These findings indicated that essential oil from seeds of C. copticum could have potential of practical value for application in management of the Indian meal moth in storage.
The Mediterranean flour moth, Ephestia kuehniella Zeller, is a major pest of stored grain products, particularly flour. There have been major concerns over the application of conventional insecticides in stored products, which have strongly demonstrated the need for applying such alternative safe compounds as essential oils. The aim of the present study is to investigate the chemical composition and fumigant toxicity of essential oil from Ziziphora clinopodioides Lam. against the eggs, larvae, and adults of E. kuehniella. All toxicity tests were carried out under laboratory conditions set at 26±1°C and 70% relative humidity (RH). The results of gas chromatography- -mass spectrometry (GC/MS) analysis indicated that the essential oil extracted from the leaves of Z. clinopodioides, is rich in pulegone (49.4%), piperitenone (10.7%), menthone (8.9%), and 1,8-cineol (6.9%). Based on the bioassay results, the LC50 value of the tested oil was estimated to be 54.61 μl · l–1 air for larvae and 1.39 μl · l–1 air for adults. Also, it is shown that increasing the oil concentration resulted in a significant increase in oviposition deterrency as well as a considerable reduction in the egg hatching percentage. These results suggest that Z. clinopodioides oil could be a potential candidate as a fumigant for managing E. kuehniella in stored products.
Plant secondary metabolites play an important role in plant-insect interactions and therefore such compounds may have insecticidal activity against insects. The chemical composition of the essential oil from leaves and flowers of Lavandula stoechas grown in Kashan, Iran, was studied by gas chromatography mass spectrometry (GC-MS). 1,8-Cineole (7.02%), γ-Cadinene (5.33%), T-Cadinol (5.07%), p-Mentha-1-en-8-ol (5.02%) and Caryophyllene (5.01%) were found to be the major constituents of the oil. In fumigant toxicity tests with the essential oil against adults of Tribolium castaneum Herbst, Lasioderma serricorne F. and Rhyzopertha dominica F. at 27±1°C and 60±5% RH, it was observed that L. serricorne (LC50 = 3.835 μl/l) were significantly more susceptible than R. dominica (LC50 = 5.66 μl/l) and T. castaneum (LC50 = 39.685 μl/l) 24 h after treatment. In all cases, considerable differences in mortality of insects to essential oil vapor were observed with different concentrations and times. Mortality increased as the doses of essential oils and exposure period increased and after 72 h fumigations, greatest percentages of mortality were obtained. The findings indicate the strong insecticidal activity of L. stoechas oil and it may be used in grain storage against insects L. serricorne, R. dominica and T. castaneum.
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