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In this study we evaluated "in vitro" the efficacy of six chemical fungicides and their mixtures as a strategy for the control of Penicillium italicum. The antifungal efficacy against four P. italicum isolates of thirty-one concentrations (0.01-3000 μg/ml) of each of the tested fungicides (Vydan, Blin exa, Canvil, Ranvil, Benomyl and Topsin M), in addition to six combined concentrations from each of ten fungicide mixtures were tested using agar well diffusion method. Regression analysis, one way ANOVA, and Post Hoc Multiple comparisons were carried out to test the significance of these treatments. Our results showed that benomyl completely inhibited the growth of tested isolates (Pi.1; Pi.3; Pi.5; and Pi.6) with MIC values of: 1000; 300; 150 and 40 μg/ml respectively. Canvil as compared to Blin exa, Ranvil and Vydan (no complete inhibition) showed high efficacy against isolates Pi.1 and Pi.5 (MIC values of 5 and 25 μg/ml respectively). The mixtures of Blin exa/Vydan and Topsin M/Canvil were the only mixtures that generated synergistic effects against tested isolates at all tested concentrations. The above mentioned mixtures showed at the first four tested combined concentrations (50:50, 100:100, 100:500 and 500:1000 μg/ml) either the largest inhibition zones (in the range of 47±1.40 mm to 51±1.49 mm) or complete inhibition of fungal growth at the last two (1000:1000 and 1000:2000 μg/ml) combined concentrations. The mixtures of Blin exa/Canvil and Blin exa/Ranvil as compared to the effect of each as singles showed additive effects against tested isolates. The mixtures of Benomyl/Vydan; Benomyl/Ranvil; Benomyl/Blin exa; Topsin/Vydan and Topsin/Blin exa (i. e. mixture of benzimidazole and DMI members) all generated antagonistic effects against tested isolates.
This study was executed to investigate the potential of agar-agar, a nontoxic and non-degradable gelling agent, as a promising coating agent to improve and protect banana fruit against fungal postharvest diseases i.e., crown, finger, neck and flower end rots which are caused by fungal isolates of Colletotrichum musae and Fusarium moniliforme. Coated-banana fruit samples with different concentrations of agar-agar suspension particularly at 2.0 g · l−1 exhibited a significant reduction in incidence and severity of postharvest diseases compared to untreated fruit. Banana fruits dipped in agar suspension at 2.0 g · l−1 for 5, 10 and 15 min showed significant reduction in disease incidence and severity. Moreover, application of agar suspension as a coating agent at 2.0 g · l−1 significantly decreased weight loss (%), firmness loss (%), and soluble solid concentration of banana fruit for 15 days at 25 ± 2°C. Scanning electron microscopy observation confirmed that the fruit coated with agar colloid at 2.0 g · l−1 had significantly fewer cracks and showed smoother surfaces than untreated fruit. This explains the quality improvement in agar-coated fruit compared to uncoated fruit. Overall, agar colloid, a safe coating agent, could be used to protect banana fruit against postharvest rot diseases and extend fruit storage life during ripening and storage.
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