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Detection of Flavobacterium psychrophilum in fry of rainbow by RT-PCR

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Vardic I., Kapetanovic D., Teskeredzic Z., Teskeredzic E.

Medycyna Weterynaryjna

2006

tom 62

nr 09

1005-1006

The paper describes the occurence of Flavobacterium psychrophilum as a concurrent pathogen with infectious hematopoietic necrosis virus in rainbow trout fry in Croatia. A molecular technique was applied for F. psychrophilum detection due to the failure of standard bacterial identification on agar plates. As the tissue material had already been used up for RNA extraction and detection of RNA virus, classical, well-described PCR could not be performed. Consequently, one step RT-PCR was adapted for F. psychrophilum identification. This method proved to be a valuable diagnostic tool for F. psychrophilum detection in mixed co-infections with RNA viruses.

Ograniczanie wyników

1 Medycyna Weterynaryjna

1 Kapetanovic D.

1 Teskeredzic E.

1 Teskeredzic Z.

1 Vardic I.

1 2006

Wyniki wyszukiwania

Detection of Flavobacterium psychrophilum in fry of rainbow by RT-PCR