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For a herbaceous species, the inverse of the fresh leaf surface density, the Hughes constant, is nearly conserved. We apply the Hughes constant to develop an absolute method of leafarea measurement that requires no regression fits, prior calibrations or oven-drying. The Hughes constant was determined in situ using a known geometry and weights of a sub-set obtained from the fresh leaves whose areas are desired. Subsequently, the leaf-areas (at any desired stratification level), were derived by utilizing the Hughes constant and the masses of the fresh leaves. The proof of concept was established for leaf-discs of the plants Mandevilla splendens and Spathiphyllum wallisii. The conservativeness of the Hughes constant over individual leaf-zones and different leaftypes from the leaves of each species was quantitatively validated. Using the globally averaged Hughes constant for each species, the leaf-area of these and additional co-species plants, were obtained. The leaf-area-measurement-by-mass was cross-checked with standard digital image analysis. There were no statistically significant differences between the leaf-area-measurement-by-mass and the digital image analysis measured leaf-areas and the linear correlation between the two methods was very good. Leaf-areameasurement- by-mass was found to be rapid and simple with accuracies comparable to the digital image analysis method. The greatly reduced cost of leaf-area-measurement-by-mass could be beneficial for small agri-businesses in developing countries.
The chemical composition of worm wood Artemisia annua L. (Astraceae), a medicinal plant, was studied by gas chromatography and gas chromatographymass spectrometry (GC-MS). About 60 compounds were identified, from which the major compounds were erythritol (50.30%), camphore (7.25%), pinocarveol (4.13%) and diethoxyethane (2.18%). The effect of crude extract on deterrency was maximal in 1000 μL/L treatment (only 4.66 ±1.05 insects were attracted to treated food) compared to control (12.6 ±0.96) The growth regulatory effect showed that 1600 μL/L treatment variously affected the weight of larvae, pupae and adult (0.48 ±0.003, 2.17 ±0.02 and 1.31 ±0.015, respectively) and finally the ovicidal effect and the number of adults appearing in F1 were similarly affected (38.5 ±1.7 and 33.75 ±2.31), comparing to control (99.25 ±1.727 and 92.5 ±1.35). The present result and previous results on this plant species indicate that it can be a good candidate for controlling stored product insects.
The fatty acid composition of waste products of Pistacia vera (pistachio tree) grown in Turkey was assayed. The waste products and various parts of the tree were classified as fresh leaves (FL), dried leaves (DL), stem (ST), branches, fresh skin of natural woody shell (unprocessed) (FSN), fresh kernel, and skin of processed woody shell. Gas chromatography-mass spectrometry data showed FSN, FL, DL and ST to be rich sources of fatty acids. In particular, FL contains a remarkable amount of linolenic acid (30.4±3.28%).
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