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Laboratory studies were carried out on the effects of an emulsifiable concentrate (EC) formulation of Azadirachtin (AZ) (6 g a.s./l EC) on four major cotton pests in Senegal: Spodoptera littoralis Boisduval (Lepidoptera: Noctuidae), Helicoverpa armigera Hϋbner (Lepidoptera: Noctuidae), Aphis gossypii Glover (Homoptera: Aphididae) and Tetranychus urticae Koch (Acari: Tetranychidae). In both S. littoralis (SL) and H. armigera (HA), larval weight loss was observed 48 h after treatment. Appreciable weight gain was only observed in SL larvae at dosages of 0.05 and 0.1 g a.s. /l AZ, 72 h after treatment. In both SL and HA, there was a significant difference (p < 0.05) between the control larvae and those treated with 0.1 to 1.5 g a.s. /l AZ. At a dosage of 1.5 g a.s./l, AZ caused 60% mortality in SL and 0% mortality in HA, 72 h after treatment. The treatment with AZ at dosages of 0.05 and 1.5 g a.s./l resulted in 50 and 30% malformation in SL and HA pupae respectively. The duration of development from the IV instar larva to pupa in both lepidopterans increased progressively with AZ dosage. SL was more susceptible to AZ than HA. No growth disrupting effects were observed in A. gossypii (AG) and T. urticae (TU) where EC50 values of 1.49 and 1.36 g a.s./l AZ respectively were obtained. The implications of the effects of AZ on further field trials against cotton pests are discussed.
The potential of immature zygotic embryos to produce embryogenic tissue was tested using culture media differed in inorganic salt composition as well as plant growth regulator content. Explants cultured on DCR as well as MLV media gave the highest initiation percentages with maximum values of 8.8% (MLV) to 10.4% (DCR). Plant growth regulators content (standard or reduced) had no profound effect on embryogenic tissue initiation. Somatic embryo maturation as well as germination was dependent on the cell line and salt composition of the medium. Plantlet regeneration occurred in three cell lines out of five tested. During the maturation process profound changes occurred in the internal organisation of somatic embryos such differentiation of root meristem in precotyledonary somatic embryos and formation of provascular strands in cotyledonary somatic embryos.
The wettable powder was prepared on the basis of aerial conidia of two isolates of the entomopathognic fungus, Beauveria bassiana. Viability and pathogenicity of conidia products were evaluated against the second-instar larva of Thrips tabaci in four cases; Conidial-product Maintained in Refrigerator (CMR), Conidial-product Maintained in Laboratory (CML), New Formulated Conidia (NFC) and New Conidia without formulation (NC). Analysis of corrected seven-day total mortality data revealed that there were significant differences among these product-cases in their pathogenicity to thrips larvae. Recorded mortality rates for CMR, CML, NFC and NC showed that the pathogenicity of CML was lower compared to three other cases for both isolates. In the next step, inorganic salts (MgCl2, NH4PO4, KH2PO4, MgSO4 and NaCl) were added at a rate of 0.1 M into the both CMR and CML products. Bioassay results indicated that caused total mortality of thrips larvae increased with adding of salts. Our results showed that applied carriers and salts have positively effected preserving of conidia viability and pathogenicity to the second-instar larva of the onion thrips.
Enhanced social concern towards the ill effects of synthetic agrochemicals, their residual toxicity and resistance development in the target pests created a necessity of exploration of alternatives. Fungal biocontrol agents have been used as herbicides over two decades and research continues to enhance their efficacy comparable to synthetic herbicides. Combinations of synthetic herbicides with the fungal biocontrol agents was an approach adopted to enhance mycoherbicidal activity but had limited acceptance due to negative effects of their synthetic components. Hence a necessity for development of the formulations having least toxicity or being benign to non-target organisms was felt. The present study brings forth a new dimension of holistic and ecofriendly bioherbicidal formulations comprising of crude phytotoxins and spores of Alternaria alternata LC# 508 which enhances outstandingly its mycoherbicidal potential. Spent broth of Alternaria alternata (LC#508) exhibited toxic activity to its spores (autotoxicity) at a concentration of 50μg/ml which was used for developing five formulations FN1 to FN5. Formulations No. 5 was the best of all the formulations when evaluated by pot trails compared to control and other formulations. Disease onset and mortality was observed in 3.5 days and 5 days respectively in case of formulation No 5 (50 μg of crude spent broth + 1x 106 spores/ml) when compared to spore spray alone. Thus the formulation of crude spent broth and fungal spores could be developed as a cost effective and efficacious bioherbicide formulation when compared to the spores alone.
Herbicidal potential of CFCF (Cell free culture filtrate) of Sclerotium rolfsii against Parthenium hysterophorus was determined by shoot cut, seedling and detached leaf bioassays. Maximum phytotoxicity was obtained from 14 day's old fermented broth with sucrose + 0.5% Tween 20 as formulating agent. Significant reduction in chlorophyll and protein contents of host was also noticed.
The new regulations of the European Parliament and European Commission (Resolution 1107/2009 and Directive 2009/128/WE) give a priority to the integrated approach to plant protection. As biological methods are essential part of such approach, their further development is important to future success in plant protection. The main changes recorded in various areas of biological control, with special reference to species of biological control agents used in practice, technologies of mass production and stability of the product, methods of formulation, biopesticides available on the market, areas of the use of biological control methods, methods of application, registration requirements, and promotion, distribution, and, advisory system are reviewed.
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