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Enzyme activity in forest peat soils

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The aim of the study was to determine the activity of dehydrogenases and urease in forest peat soils of different fertility. There were selected 23 experimental plots localised in central and northern Poland. The research was conducted on forest fens, transition bogs and raised bogs. The biggest differences in soil physical and chemical properties were detected between fen and raised bog soils while raised bog soils and transition bog soils differed the least. Statistically significant differences between particular subtypes of peat soils were observed for soil pH-H2O, pH-KCl, C/N ratio as well as the content of organic carbon, nitrogen, calcium and potassium. The highest average dehydrogenase activity in the soil surface level was observed in fen soils, and the lowest – in raised bogs soils. The results obtained on urease activity were similar. Differences in urease activity in the studied soil types were shown. Dehydrogenase activity did not reveal statistically significant diversity. The activity of urease was negatively correlated with the content of carbon, C/N ratio, hydrolytic acidity and moisture. Also the increase in enzymatic activity accompanied by the increase in pH has been observed.
Theaimof the present paper was to define the qualitative and quantitative composition of phenolic compounds in the extracts of fulvic acids and hydrolyzates of humic acids isolated from forest soil. Forest soil was sampled from the organic horizon (5-3.5 cm – sub-horizon Ol and 3.5-0 cm– sub-horizon Ofh) and mineral horizon every 10 cm (0-50 cm). Phenoliccompounds in fulvic acids (FAs) extracts and humic acids (HAs) hydrolyzates were identified by high performance liquid chromatography (HPLC). The recorded chromatograms showed differences in the composition of phenolic compounds between humic and fulvic acids fractions. The content of those compounds both in extracts of fulvic acids and hydrolyzates of humic acids and V+S+C parameter values decreased together with the sampling depth. The highest values of V+S+C parameter were calculated for extracts of fulvic acids and humic acids hydrolyzates isolated from the organic horizon of forest soil. The share of the vanillyl type in the extracts of humus acids (HAs+FAs) of the subhorizon Ol was close to that of the sum share of the syringyl and cinnamyl type. The greater the depth, the greater the share of vanillyl compounds in the pool of the share of syringyl, cinnamyl and vanillyl compounds.
Soil iron and manganese fractionation is evaluated in the soil profile sampled from a pine-covered area of Wielkopolski National Park (mid-western Poland), that for years has been exposed to acid rain. The soils studied are sands and loamy sands with a pH of 3.3-4.4. The content of iron and manganese was analyzed by sequential extraction in the following fractions: exchangeable, acid extractable, reducible, oxidizable, and residual. The soil profile displays a lithogenically dichotomous structure that is reflected in the iron and manganese content, especially in the Fe residual fraction and Mn oxidizable fraction. In terms of the lability of manganese, it can be stated that the amount of manganese in reducible fractions (Mnred) is higher than in exchangeable and acid extractable ones (except in the surface layer), while the amounts of manganese in fractions in which it is poorly available (Mnox) and unavailable (Mnres) are lower than that of Mnred. For iron, this sequence looks as follows: Feex
The aim of the study was to compare the feeding activity of soil fauna in seven temperate forests with the application of the bait-lamina method. Seven types of temperate forests located throughout Poland (East-Central Europe) were tested, ranging from dry pine forest with a typical poor quality soil to eutrophic riparian fresh deciduous forest. Each forest type was represented by five stands and all stands altogether represented natural gradient of soil fertility, texture and vegetation diversity. Despite clear diversification between the studied forest types according to a range of soil physicochemical properties and vegetation characteristics in addition to applying recommended measurement conditions for the method, we determined that the feeding activity of soil fauna did not differ between forest types. The activity of soil fauna did not depend on site botanical characteristics or any soil physical or chemical features, indicating that the bait lamina method was useless in measuring the feeding activity of soil fauna in temperate forest soils. Differences in the feeding activity of soil fauna might result from other environmental factors that influence soil fauna feeding activity in forest stands that were not measured here, i.e., soil temperature and humidity. The differences could also be attributable to the attractiveness of the bait substrate to soil fauna, which may be different in various soil conditions.
Taking into account the progressive degradation of soils it is important to assess their quality. Soil quality depends on a large number of physical, chemical, biological and biochemical properties. In the publications available, there are presented three approaches regarding the use of soil properties to estimate soil quality: (1) the use of individual properties, (2) the use of simple indexes and (3) the use of complex indexes derived from combinations of different properties. The purpose of this study was to investigate the possibility to use enzymes as indicators of forest soil quality. Experimental plots (43) were located in central Poland. The study was carried out in a number of diverse fresh forest sites. To assess the quality of forest soils dehydrogenase and urease activity and the degree of base saturation were used. One of the final conclusions point out that enzymatic activity indicates current site condition as well as the changes that occur in soil better than soil physical and chemical properties. In other words, in comparison to soil enzymatic activity, soil physico-chemical properties constitute a less sensitive indicator of soil changes.
Dehydrogenases are exclusively intracellular enzymes, which play an important role in the initial stages of oxidation of soil organic matter. One of the most frequently used methods to estimate dehydrogenase activity in soil is based on the use of triphenyltetrazolium chloride as an artificial electron acceptor. The purpose of this study was to compare the activity of dehydrogenases of forest soils with varied physicochemical properties using different triphenyltetrazolium chloride assays. The determination was carried out using the original procedure by Casida et al., a modification of the procedure which involves the use of Ca(OH)2 instead of CaCO3, the Thalmann method, and the assay by Casida et al. without addition of buffer or any salt. Soil dehydrogenase activity depended on the assay used. Dehydrogenase determined by the Casida et al. method without addition of buffer or any salt correlated with the pH values of soils. The autoclaved strongly acidic samples of control soils showed high concentrations of triphenylformazan, probably due to chemical reduction of triphenyltetrazolium chloride. There is, therefore, a need for a sterilization method other than autoclaving, ie a process that results in significant changes in soil properties, thus helping to increase the chemical reduction of triphenyltetrazolium chloride.
The aim of this study was to assess the possibility of using biological and biochemical parameters in the evaluation of forest soil quality and changes caused by land use. The study attempted to determine a relationship between the enzymatic activity of soil, the number of earthworms and soil physico-chemical properties. The study was carried out in central Poland in adjoining Forest Districts (Przedbórz and Smardzewice). In soil samples taken from 12 research plots, basic physico-chemical properties, enzyme activity (dehydrogenase, urease) and density and biomass of earthworms were examined. Enzyme activity showed a large diversity within the forest site types studied. The correlations between the activity of the enzymes studied and C/N ratio indicated considerable importance of these enzymes in metabolism of essential elements of organic matter of forest soils. Urease and dehydrogenase activity and earthworm number showed susceptibility to soil pH, which confirmed relationships between enzyme activity and abundance of earthworms and soil pH in H2O and KCl.
The aim of the present paper was to determine the content of phenolic compounds in the organic and mineral horizons of forest soils under different tree species. The study involved taking samples from the organic and mineral horizons of forest soils located in the area of the Arboretum in Mlynany, in Slovakia. Phenolic compounds were identified with the chromatographic method (HPLC). The results showed that the content of phenolic compounds identified in the forest soils depended both on the tree species and the soil sampling depth. The Ol subhorizon on the oak stand showed a similar share of vanillyl compounds to the total of syringyl and cinnamyl compounds (52:15:33), while the Ol subhorizons on the spruce and thuja stands identified a considerable advantage of vanillyl compounds. The value of parameter V+S+C, applied as the decomposition indicator of the plant material in the soil, decreased with soil profile depth and thus decreased with an increase in the degree of organic matter humification.
Assessment of the possibility of applying selected pedotransfer functions for indicating the retention of forest soils in Poland. Landscape retention influences the water balance of a catchment and the course of flooding events. The degree of retention capabilities of forest soils are much less known than the retention of land used for agriculture. Soil retention capacity influences the formation of a given forest habitat type, floristic species composition, as well as the type and quality of the stand of trees. The analysis was carried out for a regression model dedicated to selected forest soils within the area of Poland, as well as van Genuchten’s and Wösten’s, and Varallyay’s and Mironienko’s models. In order to assess the fit of the models, an independent series of forest soils were used. The models prepared for soils used for agricultural purposes do not result in statistically acceptable fit when it comes to the analyzed forest soils. The analysis of measured and calculated values of total available water indicate good fit of the regression model developed for the analyzed group of podzolic and brown forest soils.
The paper presents seasonal changes in the concentrations of ammonia, nitrate and phosphate ions in podzolic soils under the Vaccinio uliginosi–Betuletum pubescentis and Empetro nigri-Pinetum plant communities. It results from the conducted investigations that the distribution and accumulation of mineral forms of nitrogen and phosphorus are considerably affected by atmospheric conditions such as temperature, humidity and precipitation. Analysed soils were characterised by a strongly acid reaction, which decreases with the depth of the soil profiles. A statistically significant correlation was found between the content of biogenic substances in soil and litterfall. The highest concentration of biogenic substances was observed in spring and summer months. Among analysed ions the ammonia form predominated, which mean content remained within the range from 0.12 to 6.69 mg/100 g d.m. Concentration of nitrates ranged from 0.05 to 0.13 mg/100 g d.m., while that of phosphates – from 0.04 to 3.09 mg/100 g d.m. The predominance of the ammonia and phosphate forms over the nitrate form in the organic horizons may result from their retention by the sorption complex. Nitrate ions exhibit greater mobility and are leached deeper into the analysed soil profiles.
To evaluate the small-scale (100 m²) spatial variability of selected soil biological properties in a beech stand and the influence of soil properties on soil microbial activity, we collected soil samples on April 2, July 2 and October 30 (2002) from three (9 ¥ 10 m) plots, distributed on a 1 ¥ 1 m grid, from the top 10 cm of the mineral soil (A horizon). In soil samples, soil respiration and catalase activity were measured. Within-plot coefficients of variation of catalase activity varied from 19 to 23%, those of soil respiration rate ranged from 37 to 54%. Catalase activity exhibited highly significant correlations with soil reaction, whereas soil respiration rate correlated with moisture and humus content. Soil respiration rates appear to be randomly distributed over the sampling area. On the other hand, catalase activity exhibits a rather distinct patchy structure. No relationships between the position of trees and soil respiration rates were found. For catalase activities, the highest values were observed on transsections of perimeters of dominant-tree crowns.
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