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After years of scientific and public discussion concerning a temperature rise resultant from human activity and the on-going industrialisation, the global warming has become an evident fact. The effect of the temperature rise and the climate change will surely alter epidemiological aspects of some infectious diseases. In this review, we try to analyse various data concerning the impact of global warming on the spread of infectious diseases caused by bacterial, viral and protozoan agents. Certainly, it is extremely important for veterinarians and public health that some diseases have altered in their epidemiological aspects and distribution. Some new diseases may emerge; others, previously endemic, may migrate to new geographical regions. The review is focused on pathogens important to both humans and livestock, such as malaria, dengue, bluetongue, West Nile virus, tick-born diseases and infectious diarrhoeas. There are still few scientific papers on the subject, because of numerous difficulties involved in conducting such studies, such as their long time of duration, multiple factors involved in such predictions, and complicated mathematical models containing climate and epidemiological data.
Biosensor techniques are based on biospecific interaction between the biological parts of biosensor with the analyte. In biosensor construction, antibodies are usually used for the detection of analytes such as microorganism, because of very strong and highly specific interaction. The disadvantages of this assay are a long time needed for antibody isolation and purification as well as difficult regeneration of biosensor chip. The use of lectins instead of antibodies could solve these problems because a several hundred lectins are commercially available and their stability in standard buffers is better compared to monoclonal antibodies. While antibody can only be used to detect that antigen it was designed for, lectin as low affinity molecule may bind several different pathogens. Using the discriminative effect of an artificial neural network the application of a lectin array will compensate for the lower specificity. Microbial surfaces bear many of the sugar residues capable of interacting with lectins. The ability of lectins to react with microbial glycoconjugates means that it is possible to employ them as probes and sorbents for whole cells, mutants and numerous cellular constituents and metabolites, and it makes them useful tools for identification or typing of bacteria. Lectins are attractive reagents for the clinical diagnostic laboratory because of their diverse specificity, commercial availability, a wide range of molecular weights, and their stability in standard buffers. The construction of lectin biosensor could be an advantage method for detection of pathogenic bacteria.
Escherichia coli O157:H7 is well known for many foodborne outbreaks that lead to fatal infections in human being worldwide. The objective of this study was to develop a rapid and sensitive method for detection of EHEC O157:H7 from ground beef using a method that combined immunomagnetic separation (IMS) with loop-mediated isothermal amplifi cation (LAMP). The EHEC O157:H7 cells were separated with Dynabeads coated with antiEHEC O157:H7 after a short enrichment for 4 h. Then, EHEC O157:H7 was identifi ed by LAMP assay for amplifying and detecting the rfbE gene, which is highly conserved in all EHEC O157:H7 strains and exhibits strain-specific gene expression. The LAMP method results analyzed with real time turbidity measurements showed a high specificity and sensitivity, with a positive detection rate of amplifi cation of EHEC O157:H7 DNA diluted to a minimum equivalent concentration of 1.8 × 101 CFU/mL, which was 10 times more sensitive than the conventional PCR assay. The IMS followed with LAMP could capture and detect a bacterial concentration as low as 3×101 CFU/mL from the meat samples, which was close to the sensitivity of LAMP assay with pure culture. IMS combined with realistic LAMP method is a simple, rapid, highly specific gene amplifi cation technology that is suitable for implementing as a screening assay in basic laboratory and field test for detecting food contamination.
The occurrence of Campylobacter in poultry meat and subsequent antibiotic resistance profiles of the isolates were investigated. The prevalence of Campylobacter in 101 samples was 87.1%. Of these samples, 54.5% contained Campylobacter coli and 45.5% were contaminated with Campylobacter jejuni. Among the strains tested, resistance to ciprofloxacin and nalidixid acid was the most common, followed by tetracycline and streptomycin. On the other hand, all analysed isolates were susceptible to erythromycin and gentamycin. Moreover, the prevalence of several virulence marker genes among Campylobacter isolates was estimated. All strains showed the presence of the flaA and cadF factors, whereas the iam was identified only in C. jejuni, while the cdtA, cdtB, and cdtC genes were amplified almost in all C. coli isolates.
Campylobacter species are among the most frequently identified bacterial causes of human gastroenteritis. Because Campylobacter spp. harbored by cattle can be transmitted to humans, in this study we investigated antimicrobial resistance of thermophilic Campylobacter isolated from cows. Our study included 150 strains of Campylobacter (143 strains of C. jejuni and 7 strains of C. coli) isolated from cows in South-Western Poland. The minimal inhibitory concentration (MIC) to ciprofloxacin, erythromycin, gentamicin and tetracycline were determined using the agar dilution methodology. All strains of C. coli were susceptible to all four drugs studied. The most frequently detected resistance of C. jejuni was to ciprofloxacin (26 strains 18.2%). Resistance to tetracycline was observed in 5 strains (3.5%). All strains of C. jejuni were susceptible to erythromycin and gentamicin.
Significant increase in foodborn disease incidences has been reported in countries using food monitoring system. This not only poses a serious hazard to human life but also incur significant economic consequences. Therefore the European Commission has undertaken activities aimed at securing food safety and high quality standards in Europe. However, to assure food safety it will be necessary to improve contacts at the local, national and international levels among the responsible for food safety, environmental protection and pollution control.
Two hundred and forty clean, sound, unwashed eggs originating from the same farm were used a few hours after they were laid. S. enteritidis broth culture were accordingly diluted in 0.1% peptone water, and 0.1 mL of the suspension containing 104 colony forming units (CFU) was transferred to the egg surface and spread widely on the shell. The eggs were then investigated after their storage in the refrigerator or incubator at 2°, 20° or 30°C for 0, 7,14 and 21 days. Results indicate that survival of S. enteritidis on the egg shells depends, to a great extent, on the time and temperature of storage. The organisms survived better at 2°C than at 20°C and 30°C.
The occurrence of multidrug-resistant Staphylococcus aureus is an important causative agent of mastitis in cattle and of foodborne diseases. It is a worldwide concern, making it essential to develop alternative treatments to fight against the bacteria. Thus, the aim of this study is to determine the ability of carvacrol to inhibit the growth of S. aureus isolated from bulk tank milk in Turkey’s Burdur Province. All strains (n = 31) were used to investigate the antimicrobial activity of carvacrol, including the methicillin-resistant S. aureus and strains from the American Type Culture Collection and England’s National Collection of Type Cultures. The minimum inhibitory concentration (MIC) values were determined via a microdilution method, and the antimicrobial susceptibility profiles via a disk diffusion method. Antibiotic resistance was detected in 20 strains (64.5%). Multidrug resistance was observed in 8 strains (25.8%). Carvacrol exhibited strong antimicrobial activity, with MIC value at 0.058-0.234 mg/ml, in the microdilution method. Inhibition zones of carvacrol were in the range of 19 to 45 mm. The results of this study emphasize the promising role of carvacrol among new antibacterial agents that can combat S. aureus strains.
The Dsb family of redox proteins catalyzes disulfide bond formation and isomerization. Since mutations in dsb genes change the conformation and stability of many extracytoplasmic proteins, and since many virulence factors of pathogenic bacteria are extracytoplasmic, inactivation of dsb genes often results in pathogen attenuation. This study investigated the role of 2 membrane-bound oxidoreductases, DsbB and Dsbl, in the Campylobacter jejuni oxidative Dsb pathway. Campylobacter mutants, lacking DsbB or Dsbl or both, were constructed by allelic replacement and used in the human intestinal epithelial T84 cell line for the gentamicin protection assay (invasion assay) and chicken colonization experiments. In C. coli strain 23/1, the inactivation of the dsbB or dsbl gene separately did not significantly affect the colonization process. However, simultaneous disruption of both membrane-bound oxidoreductase genes significantly decreased the strain's ability to colonize chicken intestines. Moreover, C. jejuni strain 81-176 with mutated dsbB or dsbI genes showed reduced invasion/intracellular survival abilities. No cells of the double mutants (dsbB⁻ dsbI⁻) of C. jejuni 81-176 were recovered from human cells after 3 h of invasion.
This study describes a multiplex PCR assay developed for the detection of Staphylococcus aureus enterotoxin types: SEA, SEB, SEC, SED, and SEE presented in the literature as classical. The method was then used to analyse the presence of genes encoding these enterotoxins in .S aureus strains isolated from raw milk. A total of 237 raw milk samples were used in the study and 77 (32.5%) of them were found to be contaminated with S. aureus. Among them, five isolates were harbouring the genes encoding staphylococcal enterotoxins - type C (three strains) and type A (two strains). These results show that raw milk can potentially be a source of staphylococcal food poisoning.
Diarrhoea, dysentery and other diseases due to other enteric bacteria have reportedly been found to resist chemotherapeutic treatment in some West African communities with fatal consequences in some cases. This study was carried out to determine multidrug resistance patterns of Enterobacteria isolates from processed ready-to-eat foods. Indigenously processed food samples of different types were collected from two Francophone and two Anglophone countries in the West African sub-region during the wet and dry seasons of a sampling period of two years. Enterobacteria were isolated from the samples using standard techniques. Amplification of chromosomal DNA of the isolates using the Polymerase Chain Reaction was carried out. The results obtained were subjected to statistical analyses. All isolates showed resistance to cefuroxime (90.7%), nitrofurantoin (90.6%), augmentin (86.1%) and ampicillin (51.2%) while all were sensitive to gentamycin and ciprofloxacin. There was amplification indicating the presence of invA gene at a position of 240 bp. There was no amplification at all for the spvC gene in any of the isolates tested. Multidrug resistant enteric bacteria in these foods containing the invA gene could lead to infections with uncontrolled antibiotic use. The presence of enteric bacteria in the foods analyzed which provide undeniable evidence of the poor microbiological quality of these foods could form the basis of a useful databank in formulation of food-borne disease control and prevention strategies.
The aim of the study was isolation of staphylococci from milk and cream sold at the Kars market and detection of their enterotoxigenicity and possible risk for public health. A total of 160 staphylococci from 40 milk samples and 52 staphylococci from 30 cream samples were isolated. 22 (13.75%) isolates from 160 milk isolates and 9 (17.3%) isolates from 52 cream isolates were determined using human plasma as coagulase-positive staphylococci. From all the milk isolates, 4 (2.5%) of them were resistant to methycilline and one of them was resistant to vancomycin. Similarly, from all the cream samples 6 (11.54%) of them were resistance to methycilline and one (1.92%) of them was resistant to vancomycin. A total of 60 staphylococci isolates were investigated for their enterotoxigenicity. From 38 milk isolates and 22 cream isolates, 6 isolates and only one isolate respectively were found to produce staphylococcal enterotoxin (SE). One staphylococci isolated from cream samples produced only SEA, one staphylococci produced SEA, SEB, SEC, and one staphylococci produced SEA, SEB, SEC and SEE isolated from milk samples. Two isolates produced SEE and one isolate produced SEA from milk samples. In conclusion, investigated milk and cream samples are potentially the cause of staphylococcal food poisoning and can be dangerous for public health. For this reason, milk and cream should be supplied from healthy animals and milk and cream production, transporting this product to the market and keeping it their has to be done in a cold condition accordance to hygiene rules. At the same time milk producers and workers should be informed and educated of possible health risks.
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