Wyniki wyszukiwania

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The role of focal adhesion kinase in the emigration of cells from confluent cultures

100%

Trembacz H., Miloszewska J., Szaniawska B., Malecki M., Przybyszewska M., Skorski T., Janik P.

Cellular and Molecular Biology Letters

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2002

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tom 07

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nr 4

We studied the effect of the modification of focal adhesion kinase (FAK) on the growth, migration and adhesion of C3H 10T1/2 cells. Cells transfected with plasmid coding for antisense FAK displayed a low level of FAK protein. Interestingly, the transfected cells achieved a higher saturation density at confluence, and displayed reduced adhesion and enhanced emigration from a confluent layer of cells when stimulated with fibronectin. In conclusion, it can be postulated that FAK plays an important role in the mechanism of contact inhibition.

2

The C-terminal region of the focal adhesion kinase F1 domain binds akt1 and inhibits pressure-induced cell adhesion

86%

Basson M. D., Zeng B., Wang S.

Journal of Physiology and Pharmacology

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2017

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tom 68

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nr 3

3

Akt1 binds focal adhesion kinase via the Akt1 kinase domain independently of the pleckstrin homology domain

86%

Basson M. D., Zeng B., Wang S.

Journal of Physiology and Pharmacology

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2015

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tom 66

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nr 5

4

The interaction between protein kinase C epsilon and focal adhesion kinase

86%

Toton E., Hofmann J., Rybczynska M.

Cellular and Molecular Biology Letters

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2005

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tom 10

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nr Suppl.2

5

Opioids, neutral endopeptidase, its inhibitors and cancer: Is there a relationship among them?

72%

Mizerska-Dudka M., Kandefer-Szerszen M.

Archivum Immunologiae et Therapiae Experimentalis

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2015

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tom 63

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nr 3

6

alpha2beta1 integrin-mediated mechanical signals during osteodifferentiation of stem cells from the Wharton’s jelly of the umbilical cord

72%

Witkowska-Zimny M., Wrobel E., Mrowka P.

Folia Histochemica et Cytobiologica

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2014

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tom 52

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nr 4

7

Signal transduction in confluent C3H 10T1-2 cells. The role of focal adhesion kinase

72%

Miloszewska J., Trembacz H., Janik P.

Acta Biochimica Polonica

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2001

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tom 48

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nr 1

The activities of extracellular signal-regulated kinases (ERK1/ERK2) is required for proliferation of several types of cells. The performed analysis showed stimulation of ERK's by fetal calf serum (FCS) or fibronectin in the C3H 10T1/2 cell cultures at logarithmic phase of growth. The ERKs activity was not stimulated in confluent cells. This could not be accounted for a partial down regulation of ERK since its level was stable in both types of cells regardless of their density and kind of stimulation. Searching for ERK upstream elements we studied the integrin receptor gene transcript by RT-PCR and focal adhesion kinase (FAK) by Western blotting and phosphorylation assays. It was found that FCS and fibronectin stimulated phosphorylating activity of FAK in the cells at the logarithmic phase of growth, but were inefficient in the confluent cells. RT-PCR showed the presence of α5 and β1 integrin transcripts, and p125FAK was at the same level regardless of the type of stimulation. These data indicate that the ability of FAK to be activated plays an important role in ERK regulation and, in consequence in proliferation and growth inhibition during confluence.

8

Integrin receptors play a role in the internalin B-dependent entry of Listeria monocytogenes into host cells

58%

Auriemma C., Viscardi M., Tafuri S., Pavone L. M., Capuano F., Rinaldi L., Della Morte R., Iovane G., Staiano N.

Cellular and Molecular Biology Letters

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2010

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tom 15

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nr 3

496-506

Listeria monocytogenes enters non-phagocytic cells by binding its surface proteins inlA (internalin) and inlB to the host’s E-cadherin and Met, respectively. The two internalins play either separate or cooperative roles in the colonization of infected tissues. Here, we studied bacterial uptake into HeLa cells using an L. monocytogenes mutant strain (ΔinlA) carrying a deletion in the gene coding for inlA. The ΔinlA mutant strain showed the capability to invade HeLa cells. The monoclonal anti-β3- and anti-β1-integrin subunit antibodies prevented bacterial uptake into the cells, while the anti-β2- and anti-β4-integrin subunit antibodies failed to affect L. monocytogenes entry into HeLa cells. Three structurally distinct disintegrins (kistrin, echistatin and flavoridin) also inhibited bacterial uptake, showing different potencies correlated to their selective affinity for the β3- and β1-integrin subunits. In addition to inducing Met phosphorylation, infection of cells by the L. monocytogenes ΔinlA mutant strain promoted the tyrosine phosphorylation of the focal adhesion-associated proteins FAK and paxillin. Our findings provide the first evidence that β3- and β1-integrin receptors play a role in the inlB-dependent internalization of L. monocytogenes into host cells.

9

SHP-2 and PTP PEST induction during Rb-E2F associated apoptosis

58%

Morales L. D., Pena K., Kim D. J., Lieman J. H.

Cellular and Molecular Biology Letters

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2012

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tom 17

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nr 3

Apoptosis is intimately connected to cell cycle regulation via the Retinoblastoma (Rb)-E2F pathway and thereby serves an essential role in tumor suppression by eliminating aberrant hyperproliferative cells. Upon loss of Rb activity, an apoptotic response can be elicited through both p53-dependent and p53-independent mechanisms. While much of this apoptotic response has been attributed to the p19ARF/p53 pathway, increasing evidence has supported the role of protein tyrosine phosphatases (PTPs) in contributing to the initiation of the Rb-E2F-associated apoptotic response. One protein tyrosine phosphatase, PTP-1B, which is induced by the Rb-E2F pathway, has been shown to contribute to a p53-independent apoptotic pathway by inactivating focal adhesion kinase. This report identifies two additional PTPs, SHP-2 and PTP-PEST, that are also directly activated by the Rb-E2F pathway and which can contribute to signal transduction during p53-independent apoptosis.

Ograniczanie wyników

The role of focal adhesion kinase in the emigration of cells from confluent cultures

The C-terminal region of the focal adhesion kinase F1 domain binds akt1 and inhibits pressure-induced cell adhesion

Akt1 binds focal adhesion kinase via the Akt1 kinase domain independently of the pleckstrin homology domain

The interaction between protein kinase C epsilon and focal adhesion kinase

Opioids, neutral endopeptidase, its inhibitors and cancer: Is there a relationship among them?

alpha2beta1 integrin-mediated mechanical signals during osteodifferentiation of stem cells from the Wharton’s jelly of the umbilical cord

Signal transduction in confluent C3H 10T1-2 cells. The role of focal adhesion kinase

Integrin receptors play a role in the internalin B-dependent entry of Listeria monocytogenes into host cells

SHP-2 and PTP PEST induction during Rb-E2F associated apoptosis