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The inhibition of 23 strains of actinomycetes by 7 strains of fluorescent pseudomonads and vice versa, on 4 different media, was examined. The antagonism of pseudomonads was more frequent and more intense than that of actinomycetes. Inhibition was observed in pseudomonads and actinomycetes of both kinds of strains, antagonistic and non-antagonistic to Fusarium oxysporum. Some media were more favorable to the an-tagonism of pseudomonads and others to that of actinomycetes, hence in identical environmental conditions mutual antagonism between the two counter-partners occurred only rarely.
Nonfluorescent pseudomonads have been studied by estimating their numbers in different soils and hortical substrates as well as some physiological properties and antagonistic relationships between them and fluorescent pseudomonads and actinomycetes. Nonfluorescent pseudomonads, depending on the studied soil or substrate, constituted 7-10% of the total number of bacteria from genus Pseudomonas, and the dominant among them were pectinolytic psychrotrophs. Antagonism of nonfluorescent pseudomonads was less frequent and less intense than that of fluorescent pseudomonads as counter-partners. On the other hand, in the system of interrelationships with actinomycetes, the antagonism of nonfluorescent pseudomonads was relatively more frequent, though its intensity was lower.
The ability of fluorescent pseudomonads of cotton rhizosphere of induction of systemic resistance (ISR) against bacterial blight of cotton was investigated. Of the 21 isolates tested, 19 were observed to increase the resistance of plants. This was demonstrated by a lower percentage of infected leaf area. On the basis of growth promotion and ISR induction ability, isolates 148, 35Q, 16Q and 113 were selected for further investigations. All isolates increased the resistance of plants, and per cent of infected area on leaves of these treatments were lower than those control of plants. Levels of peroxidase (PO) and phenylalanine ammonia lyase (PAL) activity in the leaves of bacterized plants with selected isolates were similar to that in control plants, but after inoculation of leaves with the pathogen, the amount of these enzymes increased in bacterized plants to high levels. The increase of enzyme activity in control plants was low after inoculation.
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