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1

Computer studies of fluorescein-PE-interaction with the lipid bilayer

100%
Kubica K.
Cellular and Molecular Biology Letters
|
2002
|
tom 07
|
nr Suppl.
2
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Photoproduction of organic peroxides on the donor side of photosystem II after destruction of the water-oxidizing complex

72%
Khorobrykh A., Khorobrykh S., Yanykin D., Ivanov B., Klimov V., Mano J.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
|
2013
|
tom 94
|
nr 2
3

Estimation of the organotin compounds partition into phosphatidylcholine bilayers with a pH sensitive fluorescence probe

72%
Langner M., Kleszczynska H.
Cellular and Molecular Biology Letters
|
1997
|
tom 02
|
nr 1
The relative partition of trialkyltin and dialkyltin chlorides into the phosphatidylcholine membrane was estimated with a fluorescein dye attached to the phosphatidylethanolamine headgroup (Fluorescein-PE). Changes of pH in the vicinity of the membrane, caused by the adsorption of charged organotin compounds onto the surface, are measured with Fluorescein-PE. Measurements show that dialkyltin and trialkyltin chlorides at low concentrations (below 4 µM) adsorb onto the membrane surface in the charged form and that their relative partition depends on the length of the hydrocarbon residues. Tributyltin chloride was found to be the exception. Its effect on Fluorescein-PE fluorescence in the lipid bilayer was a complex dependence on its concentration in the sample, suggesting conformational changes in the lipid bilayer.
4

Interaction of selected anthocyanins with erythrocytes and liposome membranes

58%
Bonarska-Kujawa D., Pruchnik H., Kleszczynska H.
Cellular and Molecular Biology Letters
|
2012
|
tom 17
|
nr 2
Anthocyanins are one of the main flavonoid groups. They are responsible for, e.g., the color of plants and have antioxidant features and a wide spectrum of medical activity. The subject of the study was the following compounds that belong to the anthocyanins and which can be found, e.g., in strawberries and chokeberries: callistephin chloride (pelargonidin-3-O-glucoside chloride) and ideain chloride (cyanidin-3-O-galactoside chloride). The aim of the study was to determine the compounds’ antioxidant activity towards the erythrocyte membrane and changes incurred by the tested anthocyanins in the lipid phase of the erythrocyte membrane, in liposomes composed of erythrocyte lipids and in DPPC, DPPC/cholesterol and egg lecithin liposomes. In particular, we studied the effect of the two selected anthocyanins on red blood cell morphology, on packing order in the lipid hydrophilic phase, on fluidity of the hydrophobic phase, as well as on the temperature of phase transition in DPPC and DPPC/cholesterol liposomes. Fluorimetry with the Laurdan and Prodan probes indicated increased packing density in the hydrophilic phase of the membrane in the presence of anthocyanins. Using the fluorescence probes DPH and TMA-DPH, no effect was noted inside the hydrophobic phase of the membrane, as the lipid bilayer fluidity was not modified. The compounds slightly lowered the phase transition temperature of phosphatidylcholine liposomes. The study has shown that both anthocyanins are incorporated into the outer region of the erythrocyte membrane, affecting its shape and lipid packing order, which is reflected in the increasing number of echinocytes. The investigation proved that the compounds penetrate only the outer part of the external lipid layer of liposomes composed of erythrocyte lipids, DPPC, DPPC/cholesterol and egg lecithin lipids, changing its packing order. Fluorimetry studies with DPH-PA proved that the tested anthocyanins are very effective antioxidants. The antioxidant activity of the compounds was comparable with the activity of Trolox®.
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