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In vitro effects of compounds isolated from Sideritis brevibracteata on bovine kidney cortex glutathione reductase

100%

Tandogan B., Guvenc A., Calis I., Ulusu N. N.

Acta Biochimica Polonica

2011

tom 58

nr 4

Glutathione reductase (GR, E.C 1.6.4.2) is a flavoprotein that catalyzes NADPH-dependent reduction of oxidized glutathione (GSSG) to reduced glutathione (GSH). The aim of this study was to investigate in vitro effects of phenolic compounds isolated from Sideritis brevibracteata on bovine kidney GR. The Sideritis species are widely found in nature and commonly used as medicinal plants. 7-O-glycosides of 8-OH-flavones (hypolaetin, isoscutellarein and 3'-hydroxy-4'-O-methylisoscutellarein) were isolated from aerial parts of Sideritis brevibracteata. These compounds inhibited bovine kidney cortex GR in a concentration-dependent manner. Kinetic characterization of the inhibition was also performed.

The ability of new formamidine sugar-modified derivatives of daunorubicin to stimulate free radical formation in three enzymatic systems: NADH dehydrogenase, NADPH cytochrome p450 reductase and xanthine oxidase

100%

Pawlowska J., Tarasiuk J., Borowski E., Wasowska M., Oszczapowicz I., Wolf C. R.

Acta Biochimica Polonica

2000

tom 47

nr 1

Some sterically hindered N-substituted derivatives of daunorubicin are known to be poor substrates for NADH dehydrogenase, NADPH cytochrome P450 reductase and xanthine oxidase. In consequence, poor oxygen radical generation by these compounds is observed. In this study we examined a new family of sugar-N-substituted derivatives of daunorubicin bearing a bulky substituent introduced on the nitrogen atom through the amidine spacer. These compounds were found to be very active in radical formation catalyzed by all three studied enzymes. Thus, the introduction of a heterocyclic ring, even if it is bulky but flexible, on the nitrogen atom of daunosamine moiety through the one-atom spacer (amidine group), does not induce the steric hindrance effect on the interaction of daunorubicin derivatives with these flavoprotein enzymes.

Ograniczanie wyników

2 Acta Biochimica Polonica

1 Borowski E.

1 Calis I.

1 Guvenc A.

1 Oszczapowicz I.

1 Pawlowska J.

1 Tandogan B.

1 Tarasiuk J.

1 Ulusu N. N.

1 Wasowska M.

1 Wolf C. R.

1 2011

1 2000

Wyniki wyszukiwania

In vitro effects of compounds isolated from Sideritis brevibracteata on bovine kidney cortex glutathione reductase

The ability of new formamidine sugar-modified derivatives of daunorubicin to stimulate free radical formation in three enzymatic systems: NADH dehydrogenase, NADPH cytochrome p450 reductase and xanthine oxidase