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  1. 3 Acta Biochimica Polonica

  2. 3 Folia Histochemica et Cytobiologica

  3. 3 Journal of Physiology and Pharmacology

  4. 1 Acta Biologica Cracoviensia. Series Botanica. Supplement

  5. 1 Archivum Immunologiae et Therapiae Experimentalis

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  1. 3 Kurpisz M.

  2. 2 Fraczek M.

  3. 2 Janeczek A.

  4. 2 Kucharzewska P.

  5. 2 Rozwadowska N.

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  1. 1 2020

  2. 2 2018

  3. 1 2015

  4. 3 2014

  5. 1 2012

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1

Differentiation of sclerotomes in human embryos aged 5 weeks

100%
Grzymislawska M., Lupicka J., Wozniak W., Bruska M., Skorzewska A.
Acta Biologica Cracoviensia. Series Botanica. Supplement
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2014
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tom 56
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nr 1
2
Content available remote

Biological properties of human skeletal myoblasts genetically modified to simultaneously overexpress the pro-angiogenic factors vascular endothelial growth factor-A and fibroblast growth factor-4

100%
Zimna A., Janeczek A., Rozwadowska N., Fraczek M., Kucharzewska P., Rucinski M., Mietkiewski T., Kurpisz M.
Journal of Physiology and Pharmacology
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2014
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tom 65
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nr 2
3

FGF2-FGFR2 binding domain complex as a model system for studies of electromagnetic field effects on brain cells

100%
Estkowska J., Peplowski L., Nowak W.
Current Topics in Biophysics. Supplement
|
2011
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tom 34
|
nr A: Posters
4

Muscle stem/progenitor cells and mesenchymal stem cells of bone marrow origin for skeletal muscle regeneration in muscular dystrophies

84%
Klimczak A., Kozlowska U., Kurpisz M.
Archivum Immunologiae et Therapiae Experimentalis
|
2018
|
tom 66
|
nr 5
5

Tumor models for prostate cancer exemplified by fibroblast growth factor 8-induced tumorigenesis and tumor progression

84%
Tuomela J., Harkonen P.
Reproductive Biology
|
2014
|
tom 14
|
nr 1
6

FGF binding by extracellular matrix components of Wharton's jelly

84%
Malkowski A., Sobolewski K., Jaworski S., Bankowski E.
Acta Biochimica Polonica
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2007
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tom 54
|
nr 2
Our earlier paper has reported that Wharton's jelly is a reservoir of several peptide growth factors, including acidic and basic fibroblast growth factors (aFGF and bFGF, respectively). Both can be extracted by buffered salts solutions in the form of high molecular mass complexes, probably with a component(s) of the extracellular matrix. Both aFGF and bFGF from such extracts hardly penetrate 10% polyacrylamide gels during electrophoresis. Pre-treatment of Wharton's jelly with hyaluronidase slightly increased the extractability of aFGF, but did not affect the extractability of bFGF. In contrast, the pre-treatment of tissue homogenate with bacterial collagenase (2000 U/ml, 37°C, 18 h) increased the extractability of bFGF. The presence of β-mercaptoethanol in the extracting solutions increased the extractability of both FGFs, but did not release FGFs in their free form, despite reducing the molecular mass of the FGF-containing complexes. We conclude that both aFGF and bFGF are bound through disulphide bonds to a protein component of Wharton's jelly. We propose that ground substance composed mainly of collagen fibrils and hyaluronate molecules, which surrounds the cells of Wharton's jelly, prevents the access of the extracting solution to aFGF and bFGF. Although hyaluronate and collagen do not bind aFGF or bFGF directly, they may constitute a barrier which prevents the dispersion of FGFs in Wharton's jelly. Thus, the high concentration of FGFs around the cells of Wharton's jelly may facilitate the interaction of these factors with membrane receptors, thereby resulting in stimulation of cell division and differentiation, as well as of the synthesis of extracellular matrix components.
7

Assessment of viral and non-viral gene transfer into adult rat brains using HSV-1, calcium phosphate and PEI-based methods

67%
Corso T. D., Torres G., Goulah C., Roy I., Gambino A. S., Nayda J., Buckley T., Stachowiak E. K., Bergey E. J., Pudavar H., Dutta P., Bloom D. C., Bowers W. J., Stachowiak M. K.
Folia Morphologica
|
2005
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tom 64
|
nr 3
130-144
CNS gene transfer could provide new approaches to the modelling of neurodegenerative diseases and devising potential therapies. One such disorder is Parkinson’s disease (PD), in which dysfunction of several different metabolic processes has been implicated. Here we review the literature on gene transfer systems based on herpes simplex virus type 1 (HSV-1) and non-viral polyethyleneimine (PEI) and calcium phosphate nanoparticle methods. We also assess the usefulness of various CNS gene delivery methods and present some of our own data to exemplify such usefulness. Our data result from vectors stereotaxically introduced to the substantia nigra (SN) of adult rats and evaluated 1 week and/or 1 month post injection using histochemical methods to assess recombinant β-galactosidase enzyme activity. Gene transfer using PEI or calcium phosphate-mediated transfections was observed for both methods and PEI was comparable to that of HSV-1 amplicon. Our data show that the amplicon delivery was markedly increased when packaged with a helper virus and was similar to the expression profile achieved with a full-size replication-defective HSV-1 recombinant (8117/43). We also examine whether PEI or HSV-1 amplicon-mediated gene transfer could facilitate assessment of the biological effects induced by a dominant negative FGF receptor-1 mutant to model the reduced FGF signalling thought to occur in Parkinson’s disease.
8
Content available remote

Local regulators of seasonal reproduction processes in uterus masculinus of an adult male European bison (Bison bonasus, Linnaeus 1758)

67%
Tabecka-Lonczynska A., Mytych J., Solek P., Abrachamowicz A., Welz M., Koziorowski M.
Journal of Physiology and Pharmacology
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2018
|
tom 69
|
nr 5
9
Content available remote

Transient and stable transfections of mouse myoblasts with genes coding for pro-angiogenic factors

67%
Bialas M., Krupka M., Janeczek A., Rozwadowska N., Fraczek M., Kotlinowski J., Kucharzewska P., Lackowska B., Kurpisz M.
Journal of Physiology and Pharmacology
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2011
|
tom 62
|
nr 2
Cardiomyocyte loss in the ischaemic heart can be the reason of many complications, eventually being even the cause of patient's death. Despite many promises, cell therapy with the use of skeletal muscle stem cells (SMSC) still remains to be modified and improved. Combined cell and gene therapy seems to be a promising strategy to heal damaged myocardium. In the present study we have investigated the influence of a simultaneous overexpression of two potent pro-angiogenic genes encoding the fibroblast growth factor-4 (FGF-4) and the vascular endothelial growth factor-A (VEGF-A) on a myogenic murine C2C12 cell line. We have demonstrated in in vitro conditions that myoblasts which overexpressed these factors exhibited significant changes in the cell cycle and pro-angiogenic potential with only slight differences in the expression of the myogenic genes. There was not observed the influence of transient or stable overexpression of FGF-4 and VEGF on cell apoptosis/necrosis in standard or oxidative stress conditions comparing to non transfected controls. Overall, our results suggest that the possible transplantation of myoblasts overexpressing pro-angiogenic factors may potentially improve the functionality of the injured myocardium although the definite proof must originate from in situ conducted pre-clinical studies.
10
Content available remote

Modulatory effect of gastric pentadecapeptide BPC 157 on angiogenesis in muscle and tendon healing

67%
Brcic L., Brcic I., Staresinic M., Novinscak T., Sikiric P., Seiwerth S.
Journal of Physiology and Pharmacology. Supplement
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2009
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tom 60
|
nr 7
11

Search of reference biomarkers reflecting orbital tissue remodeling in the course of Graves’ orbitopathy

67%
Pawlowski P., Poplawska I., Mysliwiec J., Dik W. A., Eckstein A., Berchner-Pfannschmidt U., Milewski R., Lawicki S., Dzieciol-Anikiej Z., Rejdak R., Reszec J.
Folia Histochemica et Cytobiologica
|
2020
|
tom 58
|
nr 1
12

mRNA and protein expression of FGF-1, FGF-2 and their receptors in the porcine umbilical cord during pregnancy

67%
Chrusciel M., Rekawiecki R., Ziecik A., Andronowska A.
Folia Histochemica et Cytobiologica
|
2010
|
tom 48
|
nr 4
13

Properties of B16-F10 murine melanoma cells subjected to metabolic stress conditions

67%
Mitrus I., Bryndza E., Kazura M., Smagur A., Sochanik A., Cichon T., Szala S.
Acta Biochimica Polonica
|
2012
|
tom 59
|
nr 3
Neoplastic cells which co-form tumors are usually subjected to various stress factors, mainly hypoxia and shortage of nutrient factors. Such cells employ different strategies that permit their survival under such conditions. Experiments in vitro are usually carried out in the presence of 21% oxygen and medium supplemented with 10% FBS. Altering these parameters can approximate the in vivo conditions found within tumor mass. The present paper reports certain properties (especially ability to metastasize) of B16-F10 cells able to grow upon exposure to altered growth conditions (medium supplemented with 0.06% FBS or presence of 1% oxygen for 24 or 72 hours). These properties were compared with those of control cells cultured in the presence of 21% oxygen and in medium supplemented with 10% FBS. Some properties of the cells exposed to medium supplemented with 0.06% FBS differ from those of cells cultured under low oxygenation conditions (ability to form metastases, to migrate, or to express various proteins). Only the partial deprivation of oxygen did increase both the number of migrating cells and the number of metastases formed. Serum deficiency enhanced only the cell ability to metastasize, but not to migrate. It appears that cultured B16-F10 cells employ different adaptation strategies under conditions of oxygen shortage and those of serum deficiency. Under oxygen deprivation, such cells most likely undergo an epithelial-mesenchymal transition, whereas serum deficiency ("starvation"), while increasing the tumorigenicity of B16-F10 cells, does not induce the epithelial-mesenchymal transition.
14

Metallothioneins in the lung cancer

67%
Werynska B., Pula B., Kobierzycki C., Dziegiel P., Podhorska-Okolow M.
Folia Histochemica et Cytobiologica
|
2015
|
tom 53
|
nr 1
15

Growth factor/growth factor receptor loops in autocrine growth regulation of human prostate cancer DU145 cells

59%
Ligeza J., Ligeza J., Klein A.
Acta Biochimica Polonica
|
2011
|
tom 58
|
nr 3
Autocrine growth factors produced by epithelial cells mediate the development and proliferation of neoplastic human prostate tissue. Various approaches have been used to down-regulate neoplastic growth of prostate cancer using natural flavonoids, soluble receptors, pseudo-ligands, monoclonal antibodies and tyrosine kinase inhibitors (tyrphostins). Selected growth factor/growth factor receptor loops (mainly TGFα/EGFR and IGFs/IGFIR) have been proposed as regulators of prostate cancer cell growth. We have previously determined that blockade of IGFIR or VEGF2R signaling pathways by tyrphostin AG1024 and SU1498 inhibits autocrine growth and viability of DU145 cells in vitro. Recently, we compared the activity of AG1024 and SU1498 with the inhibiting effect of tyrphostin A23 (a selective inhibitor of EGFR). The results described in this paper confirm that DU145 cells do not produce IGFI or EGF. In contrast, DU145 cells produce a great amount of VEGF, much more than TGFα (about 60-fold), and VEGF may be the real autocrine growth factor of the investigated cells. The results indicate that the growth of DU145 may be regulated by at least three autocrine loops: TGFα/EGFR, IGFII/IGFIR and VEGF/VEGFR2. Neither AG1024 nor SU1498 affected the production of TGFα substantially, which excludes the possibility that IGFRs or VEGFR2 inhibitors arrest the growth of these cells by inhibition of synthesis and/or secretion of TGFα. The obtained data indicate that all tree investigated tyrphostins (AG1024, SU1498 and A23) inhibit signal transmission by Akt (PKB), ERK(1/2), Src and STAT in a similar manner. A comparison of the effects of the investigated tyrphostins indicates that TGFα, IGFII and VEGF stimulate cell growth by affecting the same signaling pathway. The hypothesis was confirmed by the effect of the investigated tyrphostins on activation of EGFR. All these inhibitors decreased phosphorylation of EGFR to the same extent, and after the same time of incubation with cell culture. These results strongly suggest that stimulation of EGFR kinase is the main step in the initiation of mitogen signaling in DU145 cells, regardless of the type of ligand (TGFα, IGFs or VEGF) and their specific receptors.
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