Explants of young leaves and hypocotyls of M. crystallinum were cultured on a Murashige and Skoog's (MS) medium containing auxins, cytokinins and auxin-cytokinin combinations. Callus was obtained from the medium supplemented with kinetin and 2,4-D. Callus induction was more readily obtained from hypocotyls than from leaves. Media containing zeatin (ZT), kinetin (KT), 6-benzylaminopurine (BA) and 2,4-dichlorophenoxyacetic acid (2.4-D) or a-naphthalene acetic acid (NAA) were used for bud multiplication and shoot differentiation. Shoot formation required cytokinins. The combination of cytokinins with low concentrations of auxin improved bud multiplication and shoot formation. The regenerated shoots developed roots on growth regulator free MS medium. Complete plantlets grew in the greenhouse to maturity after transfer to soil, and seeds could be collected.
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