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1

Use of complementary feeding stuff Ichtio Hexan to control Spironucleus salmonis infections in rainbow trout

100%
Puk K., Guz L., Sopinska A.
Medycyna Weterynaryjna
|
2014
|
tom 70
|
nr 10
The aim of this study was to determine the efficacy of Ichtio Hexan (a complex blend of Allium sativum extract, Chelidonium majus extract, Origanum vulgare extract, carvacrol and cinnamic aldehyde) for the control of spironucleosis (hexamitosis) in rainbow trout fingerlings. For this purpose, three groups of rainbow trout naturally infected with S. salmonis were fed diets containing either no Ichtio Hexan or supplemented with Ichtio Hexan at a rate of 0.1 ml/kg bw (group 1) or 1 ml/kg bw (group 2) for 38 days. During this period mortalities were recorded and the dead fish were examined to confirm the isolations of S. salmonis. At the end of the experiment the fish were sacrificed, individually weighed and measured, their livers removed and weighed. In order to determine the prevalence and intensity of infection the intestinal contents were examined. The results of the present study showed that Ichtio Hexan in an amount of 0.1 ml/kg bw considerably reduced mortality caused by S. salmonis in rainbow trout fingerlings. The number of S. salmonis trophozoites in the digestive tract after administration of Ichtio Hexan at this dose for 38 days was significantly reduced and limited only to the posterior part of the intestine. On the other hand, in the 1 ml/kg bw fed group none of the fish were infected and the gain in the body weight was significantly increased; however, the mortality rate was similar to the control group. The experimental groups did not differ significantly from each other with regards to the condition factor and hepatosomatic index. Considering the above findings, Ichtio Hexan at a dose of 0.1 ml/kg bw can be successfully used in rainbow trout farms to reduce the mortality rate in S. salmonis infected fish.
2

Prevalence of genetically modified soybean in animal feedingstuffs in Poland

100%
Sieradzki Z., Mazur M., Krol B., Kwiatek K.
Journal of Veterinary Research
|
2021
|
tom 65
|
nr 1
3

In-house validation method for quantification of amoxicillin in medicated feedingstuffs with the use of HPLC-DAD technique

100%
Patyra E., Kwiatek K.
Journal of Veterinary Research
|
2020
|
tom 64
|
nr 3
4

Occurrence of tetracyclines in feedingstuffs – results of a two-year study within the official control of feed

100%
Przenioslo-Siwczynska M., Patyra E., Chylek-Purchala M., Kozak B., Kwiatek K.
Bulletin of the Veterinary Institute in Puławy
|
2015
|
tom 59
|
nr 4
The paper describes a microbiological method for the detection of antibacterial substances in feedingstuffs. The method allowed detection of the main antibiotic groups, including tetracyclines. In 2013-2014, a total of 171 feed samples were analysed to determine antibacterial substances. Among the analysed samples 84 (49.1%) were suspected to contain tetracyclines. Out of the 84 feeds analysed using chromatography, 28 (33.3%) contained undeclared tetracyclines, which were identified at concentrations ranging from 0.32 mg kg⁻¹ to 48.98 mg kg⁻¹.
5

Microbiological quality of animal feedingstuffs in Poland

100%
Wojdat E., Kwiatek K., Kozak M.
Bulletin of the Veterinary Institute in Puławy
|
2005
|
tom 49
|
nr 3
6

The evaluation of the efficacy of sodium carbonate as zearalenone destructor in feeding stuffs

84%
Polak M., Gajecki M., Kulik T., Luczynski M. K., Obremski K., Gora M., Gajecka M., Jakimiuk E., Zielonka L.
Polish Journal of Veterinary Sciences
|
2009
|
tom 12
|
nr 1
Contamination of feed with zearalenone (ZEA) is still a serious problem in farm animals feeding, especially in gilts, sensitive to this compound. The relative failure of current methods of decontamination and quality control lead us to look for new techniques. The commonly accepted method for breaking down ZEA was performed in controlled temperature and time conditions. Various sodium carbonate doses (0.5 – 4%) were added to feed naturally contaminated with ZEA (ZEA biosynthesis by F. graminearum isolates). These doses were found to be effective in in vitro studies. The addition of 2% sodium carbonate gave the best results in reducing the phytoestrogen in the feed.
7
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Detection and identification of banned processed animal protein in feedingstuffs by microscopic and PCR methods

84%
Weiner A., Golebiowska A., Paprocka I., Kwiatek K.
Polish Journal of Veterinary Sciences
|
2012
|
tom 15
|
nr 1
The aim of the study was to present the results of comparative evaluation of the usefulness of PCR and microscopic methods for the detection of Processed Animal Protein (PAP) in feedingstuffs. In the validation study, the limit of the detection for PCR was determined on 0.05% for beef, 0.1% for pork and 0.2% for poultry meat and bone meal (MBM). Among 62 doubtful samples of feedingstuffs examined by microscopic method 41 (66.13%) were found as positive. Based on the results obtained with the use of the microscopic and PCR methods it is possible to state that the molecular biology methods can, at present, be used as a supplementary method in PAP detection.
8

Occurrence of Clostridium perfringens in food chain

67%
Kukier E., Kwiatek K.
Bulletin of the Veterinary Institute in Puławy
|
2010
|
tom 54
|
nr 4
The occurrcnce of Clostridium perfringens in particular links of food chain and their toxic potential was studied. Compound feeding stuffs, poultry, swine, and bovine faeces, food of animal origin, and human faeces were examined microbiologically. The isolated anaerobes were analysed for presence of toxin genes (cpa, cpb, cpb2, etx, iap, and cpe). The presence of C. perfringens on level higher than 1.0 x 10¹ cfu/g were detected in 68% of feed samples, 36% of poultry faeces, 92% of swine faeces, 67% of bovine faeces, 4% of food samples, and in 67% of human faeces. The detected levels of type A C. perfringens strains did not exceed the number of these strains physiologically present in the intestines of birds and mammals. Food and feed samples also revealed good microbiological quality. The identification of toxin type and subtype revealed domination of type A strains and among them the percentage of subtype ß2 strains varied considerably. The second toxotype detected was toxotype E. There were no cpe-positive strains in the analysed food of animal origin.
9

Phenotypical and genotypical characterisation of Clostridium perfringens strains isolated from feedingstuffs

67%
Kukier E., Kwiatek K.
Bulletin of the Veterinary Institute in Puławy
|
2010
|
tom 54
|
nr 4
In this study, Clostridium perfringens strains isolated from feedingstuffs were characterised phenotypically (width of lecithinolysis and partial haemolysis zones, biochemical reactions and resistance to antibacterial substances) and genotypically (presence of toxin genes and genomic polymorphism analysis). C. perfringens toxin genes were detected by multiplex PCR and genomic polymorphism was analysed by pulsed field gel electrophoresis. It was demonstrated that C. perfringens strains type A, among which more than half belonged to subtype ß2, were the most commonly isolated strains. Strains containing cpb2 gene revealed higher diversity of their biochemical reactions, more often occurring antibiotic resistance, and lower genomic DNA profile similarity. Feedingstuffs may serve as a source of C. perfringens, the most important animal pathogen among anaerobic sporulating bacteria. Widely occurrence of cpb2 gene among C. perfringens isolates from feedingstuffs may suggest taking into account ß2 toxin in animal immunoprophylaxis. The knowledge gained in this study shows feedingstuffs as a source of C. perfringens ß2 toxin in food chain and sheds light on the animal immunoprophylaxis regarding diseases caused by this microorganism.
10

Zasady zywienia krow mlecznych systemem TMR

67%
Mroczek R.
Biuletyn Informacyjno-Handlowy. Ośrodek Doradztwa Rolniczego Boguchwała
|
2003
|
nr 12
13-14
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