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To estimate the current prevalence of gastrointestinal (GI) parasites in dogs and cats, a total of 105 fresh faecal samples were collected from rural areas in Peninsular Malaysia. Each faecal sample was examined for the presence of GI parasites by microscopic examination after formalin-ether concentration technique and for protozoa, trichrome and Ziehl-Neelsen staining were employed. The overall prevalence of GI parasitic infection was 88.6% (95% CI = 82.5–94.7) in which 88.3% of dogs and 89.3% of cats were infected with at least one parasites species, respectively. There were 14 different GI parasites species (nematodes, cestodes and protozoa) detected, including Ancylostoma spp. (62.9%), Toxocara spp. (32.4%), Trichuris vulpis (21.0%), Spirometra spp. (9.5%), Toxascaris leonina (5.7%), Dipylidium caninum (4.8%), Ascaris spp. (2.9%), Hymenolepis diminuta (1.0%) and others. General prevalence of GI parasites showed a significant difference between helminth (84.4%) and protozoa (34.3%) infections. Monoparasitism (38.1%) was less frequent than polyparasitism (46.7%). As several of these GI parasites are recognized as zoonotic agents, the results of this investigation revealed that local populations may be exposed to a broad spectrum of zoonotic agents by means of environmental contamination with dogs and cats faeces and this information should be used to mitigate public health risks. Prevention and control measures have to be taken in order to reduce the prevalence rates especially in socioeconomically disadvantaged communities where animals live in close proximity to people, poor levels of hygiene and overcrowding together with a lack in veterinary attention and zoonotic awareness.
Prey occurrence from stomach and fecal samples were compared in American martenMartes americana Turton, 1806, and fisherM. pennanti Erxleben, 1777. Diets were analyzed from stomach and fecal contents in four sex-age marten groups and four fisher groups, and dietary differences tested between species, sex, age and sample sources. Prey richness was compared between the two sample sources. Relative occurrence of dominant or co-dominant prey items did not differ between stomach and fecal samples in any of the groups. However, both series revealed inter-specific contrasts. Binomial correlations of prey occurrence between sample sources were significantly negative for most prey items. Correlations of prey richness between samples sources were not significant except in larger marten sample. Our results failed to reveal a bias in using fecal sample source. Thus we suggest that diet analyses using either sample source are valid when diets are assessed in a relatively large number of animals.
Biological material was taken from dogs with diarrhea. Faecal samples were taken from live animals white intestinal tract fragments (i.e. small intestine, and stomach) were taken from dead animals. In total, 18 specimens were investigated from dogs housed alone or in large groups. The samples were examined for presence of viral infections and concurrent bacterial and parasitic infestations. To test for the presence of the viral infection, latex (On Site Biotech, Sweden) and direct immunofluorescence tests were performed. At the same time to the presence of CPV infection, was conducted by the PCR method with primers complementary to a conservative region of VP1/VP2. In order to identify the bacterial strain, the material was inoculated onto appropriate media and identified with API tests, whilst parasitological examinations were performed with Fulleborn's method. CPV infection was accompanied by CCV and CAV infections, as well as bacterial ones, caused mostly by Escherichia coli.
Amoebiasis is one of the most important infectious diseases afflicting mainly tropical and subtropical countries. This study was carried out in the Sharjah Emirate, UAE in order to accurately detect and differentiate Entamoeba histolytica, Entamoeba dispar and E. moshkovskii in fecal samples collected from the Sharjah municipality public health clinic by ELISA and nested polymerase chain reaction (PCR). One hundred and twenty specimens were examined and the PCR was positive for E. histolytica, E. dispar and E. moshkovskii (collectively referred to as Entamoeba complex) in 19.2% (23 out of 120). Of those, 10% (12/120) were mono — infection with E. histolytica; 2.5% (3/120) with E. dispar; and 2.5% (3/120) E. moshkovskii. The nested PCR also detected mixed infections by both E. histolytica and E. dispar in 3.3% (4/120) and E. dispar and E. moshkovskii in 0.8% (1/120). The TechLab ELISA kit failed to detect E. histolytica in any of the E. histolytica PCR positive samples. Overall, the percentage of E. histolytica including those found in mixed infections was 13.3% (16/120). Compared to nested PCR, microscopy was found to have an overall sensitivity of 52.2% and a specificity of 75.2% for detection of Entamoeba complex. The present study indicates that E. histolytica is present in the UAE with an average incidence rate of 13.3%. However, larger studies need to be conducted in order to confirm these findings. We propose the use of PCR in both the routine diagnosis of amoebiasis and epidemiological survey in the UAE.
Prevalence of intestinal parasites in dogs and cats in Barcelona and surrounding areas was studied by analyzing 505 faecal samples of dogs and 50 of cats using a formol-ether diphasic method for helminths, a modified acid-fast technique for Cryptosporidium and other coccidian oocysts, and the Heindenhein technique for Giardia and Entamoeba trophozoites and cysts. Parasites were found in 26.9% of dogs and 34.0% of cats. Giardia duodenalis, Cryptosporidium sp., coccidian oocysts and Entamoeba sp. were detected in both dogs and cats. Taenia sp., Dipylidium caninum, Ancylostoma caninum, Toxocara canis, Toxascaris leonina, Trichuris vulpis and Uncinaria sp. were also found in dogs but only Ancylostoma tubaeforme and Toxocara cati in cats. There was a significant relationship for G. duodenalis and Cryptosporidium sp. with seasonality and for G. duodenalis with geographical origin in dogs. Significant differences were also found for T. canis between stray and domestic dogs and for T. vulpis between males and females.
This study was performed to determine the tapeworm infection of grazing cattle in 11 dairy herds in Lower Silesia and Lesser Poland (Galicia). Rectal faecal samples were examined microscopically for tapeworm eggs by Willis-Shlaaf’s flotation, Telemann’s sedimentation (for fatty stools) and decantation method. Out of 182 cattle, 10 (5.5%) were found to be infected with tapeworms. The prevalence in 5 of 11 examined herds varied from 15.5 to 30.5% and was highest for animal being in the first lactation. Tapeworm eggs were detected in animals being in the first and second lactation (about three- to four-years-old cows). All the qualitative methods were effective in the diagnosis of tapeworm infection in cattle.
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Giardia prevalence in wild cervids in Poland

58%
A total of 181 faecal samples were collected from wild cervids in two regions of Poland. Specimens were taken from 65 fallow deer (Dama dama), 61 red deer (Cervus elaphus), 50 roe deer (Capreolus capreolus), and five moose (Alces alces). Giardia cysts were detected in one faecal specimen from the red deer and in two samples from the roe deer. Although this study has demonstrated that Giardia infection is remarkably rare in wild cervids, it should be emphasized that there are large populations of these animals in Poland.
Cryptosporidium parvum is endemic to South Africa. In order to provide direction for prevention strategies, information on the dominant genotype involved in human infections is required. A combination of diethyl ether extraction, sodium chloride flotation and immuno-magnetic separation (IMS) was used for maximum recovery of oocysts from faecal samples. In order to improve the sensitivity of PCR-RFLP analysis of the Cryptosporidium oocyst wall protein (COWP) gene, a nested PCR was developed. RFLP analysis was done on a 553 bp segment obtained from the final nested PCR reaction to distinguish between C. parvum genotype 1 (human exclusive type) and genotype 2 (broad host range). Overall, genotype 2 was detected in 10 samples, whereas genotype 1 was detected in the remaining 40 samples. The high incidence of genotype 1 parasites (80%) implies hat direct and indirect human transmission plays a dominant role in the epidemiology of the disease caused by this parasite in the study area.
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