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  1. 4 Cellular and Molecular Biology Letters

  2. 3 Acta Biochimica Polonica

  3. 2 Herba Polonica

  4. 1 Acta Ichthyologica et Piscatoria

  5. 1 Acta Parasitologica

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  1. 3 Pawelczyk T.

  2. 3 Sakowicz M.

  3. 3 Zhang H.

  4. 2 Bogacz A.

  5. 2 Czerny B.

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  1. 2 2019

  2. 1 2017

  3. 2 2013

  4. 1 2012

  5. 3 2011

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1

The effect of calnexin deletion on the expression level of PDI in Saccharomyces cerevisiae under heat stress conditions

100%
Zhang H., He J., Ji Y., Kato A., Song Y.
Cellular and Molecular Biology Letters
|
2008
|
tom 13
|
nr 1
38-48
We cultured calnexin-disrupted and wild-type Saccharomyces cerevisiae strains under conditions of heat stress. The growth rate of the calnexin-disrupted yeast was almost the same as that of the wild-type yeast under those conditions. However, the induced mRNA level of the molecular chaperone PDI in the ER was clearly higher in calnexin-disrupted S. cerevisiae relative to the wild type at 37°C, despite being almost the same in the two strains under normal conditions. The western blotting analysis for PDI protein expression in the ER yielded results that show a parallel in their mRNA levels in the two strains. We suggest that PDI may interact with calnexin under heat stress conditions, and that the induction of PDI in the ER can recover part of the function of calnexin in calnexin-disrupted yeast, and result in the same growth rate as in wild-type yeast.
2
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Identification of new Arabidopsis thaliana pri-miRNAs and mature miRNAs responsive to drought conditions

100%
Barciszewska-Pacak M., Skorupa K., Bielewicz D., Dolata J., Jarmolowski A., Szweykowska-Kulinska Z.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
|
2013
|
tom 94
|
nr 3
3
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Association of alpha-A globin gene polymorphism with its expression level in racing pigeons

100%
Jedrzejczak-Silicka M., Dudaniec K., Dybus A.
Acta Scientiarum Polonorum. Zootechnica
|
2019
|
tom 18
|
nr 1
4

Expression level of TRPM7 channel in mammary epithelial cells selected to grow in very low [0.025 mM] and very high [45 mM] magnesium containing media

100%
Simonacci M., Trapani V., Boninsegna A., Cittadini A., Wolf F. I.
Journal of Elementology
|
2005
|
tom 10
|
nr 4 Suppl.1
97-98
5

Expression level of Ubc9 protein in rat tissues

100%
Golebiowski F., Szulc A., Sakowicz M., Szutowicz A., Pawelczyk T.
Acta Biochimica Polonica
|
2003
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tom 50
|
nr 4
Ubc9 is a homologue of the E2 ubiquitin conjugating enzyme and participates in the covalent linking of SUMO-1 molecule to the target protein. In this report we describe a simple and efficient method for obtaining pure human recombinant Ubc9 protein. The purified Ubc9 retained its native structure and was fully active in an in vitro sumoylation assay with the promyelocytic leukaemia (PML) peptide as a substrate. In order to better understand the physiology of Ubc9 protein we examined its levels in several rat tissues. Immunoblot analyses performed on tissue extracts revealed quantitative and qualitative differences in the expression pattern of Ubc9. The Ubc9 protein was present at a high level in spleen and lung. Moderate level of Ubc9 was detected in kidney and liver. Low amount of Ubc9 was observed in brain, whereas the 18 kDaband of Ubc9 was barely visible or absent in heart and skeletal muscle. In heart and muscle extracts the Ubc9 antibodies recognized a 38 kDa protein band. This band was not visible in extracts of other rat tissues. A comparison of the relative levels of Ubc9 mRNA and protein indicated that the overall expression level of Ubc9 was the highest in spleen and lung. In spleen, lung, kidney, brain, liver and heart there was a good correlation between the 18 kDa protein and Ubc9 mRNA levels. In skeletal muscle the Ubc9 mRNA level was unproportionally high comparing to the level of the 18 kDa protein. The presented data indicate that in the rat the expression of the Ubc9 protein ap­pears to have some degree of tissue specificity.
6
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Effect of Camellia sinensis extract on the expression level of transcription factors and cytochrome P450 genes coding phase I drug-metabolizing enzymes

84%
Bogacz A., Karasiewicz M., Bartkowiak-Wieczorek J., Ozarowski M., Seremak-Mrozikiewicz A., Kujawski R., Mikolajczak P. L., Mrozikiewicz-Rakowska B., Bobkiewicz-Kozlowska T., Czerny B., Grzeskowiak E., Mrozikiewicz P. M.
Herba Polonica
|
2013
|
tom 59
|
nr 4
Green tea (Camellia sinensis) is widely used as a popular beverage and dietary supplement that can significantly reduce the risk of many diseases. Despite the widespread use of green tea, the data regarding the safety as well as herb-drug interactions are limited. Therefore, the aim of our study was to assess the influence of standardized green tea extract (GTE) containing 61% catechins and 0.1% caffeine on the expression level of rat CYP genes and the corresponding transcription factors expression by realtime PCR. The findings showed that GTE resulted in a significant decrease of CYP2C6 expression level by 68% (p<0.001). In case of CYP3A1 and CYP3A2, the mRNA levels were also reduced by extract but in a lesser degree compared to CYP2C6. Simultaneously the significant increase in the mRNA level of CAR, RXR and GR factors was observed by 54% (p<0.05), 79% (p<0.001) and 23% (p<0.05), respectively after 10 days of green tea extract administration. In addition, there was noted a small increase of CYP1A1 expression level by 21% (p>0.05) was noted. No statistically significant differences were observed for CYP1A2 and CYP2D1/2. In the same study we observed an increase in amount of ARNT gene transcript by 27% (p<0.05) in the long-term use. However, green tea extract showed the ability to stimulate HNF-1α both after 3 and 10 days of treatment by 30% (p<0.05) and 80% (p<0.001), respectively. In contrast, no change was observed in the concentration of HNF-4α cDNA. These results suggest that GTE may change the expression of CYP enzymes, especially CYP2C6 (homologue to human CYP2C9) and may participate in clinically significant interactions with drugs metabolized by these enzymes.
7
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Impact of Curcuma longa extract on the expression level of brain transporters in in vivo model

84%
Bukowska M., Bogacz A., Wolek M., Mikolajczak P. L., Olbromski P., Kaminski A., Czerny B.
Herba Polonica
|
2019
|
tom 65
|
nr 1
Introduction: Blood brain barrier and multidrug resistance phenomenon are subjects of many investigations. Mainly, because of their functions in protecting the central nervous system (CNS) by blocking the delivery of toxic substances to the brain. This special function has some disadvantages, like drug delivery to the brain in neurodegenerative diseases. Objective: The aim of this study was to examine how natural and synthetic substances affect the expression levels of genes (Mdr1a, Mdr1b, Mrp1, Mrp2, Oatp1a4, Oatp1a5 and Oatp1c1) that encode transporters in the blood-brain barrier. Methods: cDNA was synthesized from total RNA isolated from rat hippocampus. The expression level of genes was determined using real-time PCR (RT-PCR) method. Results: Our findings showed that verapamil, as a synthetic substance, caused the greatest reduction of mRNA level of genes studied. The standardized extract of Curcuma longa reduced the expression level for Mrp1 and Mrp2, whereas the increase of mRNA level was observed for Mdr1b, Oatp1a5 and Oatp1c1. Conclusions: These results suggests that herbal extracts may play an important role in overcoming the blood brain barrier during pharmacotherapy
8

Autophagy favors Brucella melitensis survival in infected macrophages

84%
Guo F., Zhang H., Chen C., Hu S., Wang Y., Qiao J., Ren Y., Zhang K., Wang y., Du G.
Cellular and Molecular Biology Letters
|
2012
|
tom 17
|
nr 2
This study investigated the role of autophagy in the survival of the invasive bacterium Brucella melitensis strain 16M in murine macrophages. Here, Brucella melitensis 16M was found to trigger autophagosome formation, enhance autophagy flux and increase the expression level of the autophagy marker protein LC3-II. When autophagy was pharmacologically inhibited by 3-methyladenine (3-MA), Brucella replication efficiency was significantly decreased (p < 0.05). These results suggest that autophagy favors Brucella melitensis 16M survival in murine macrophages.
9

Comparative analysis of codon usage pattern and its influencing factors in Schistosoma japonicum and Ascaris suum

84%
Mazumder G. A., Uddin A., Chakraborty S.
Acta Parasitologica
|
2017
|
tom 62
|
nr 4
10

The effect of calnexin deletion on the expression level of binding protein [BiP] under heat stress conditions in Saccharomyces cerevisiae

84%
Zhang H., Hu B., Ji Y., Kato A., Song Y.
Cellular and Molecular Biology Letters
|
2008
|
tom 13
|
nr 4
621-631
We cultured calnexin-disrupted and wild-type Saccharomyces cerevisiae strains under conditions of heat stress. The growth rate of the calnexin-disrupted yeast was almost the same as that of the wild-type yeast under those conditions. However, the induced mRNA level of the molecular chaperone PDI in the ER was clearly higher in calnexin-disrupted S. cerevisiae relative to the wild type at 37°C, despite being almost the same in the two strains under normal conditions. The western blotting analysis for PDI protein expression in the ER yielded results that show a parallel in their mRNA levels in the two strains. We suggest that PDI may interact with calnexin under heat stress conditions, and that the induction of PDI in the ER can recover part of the function of calnexin in calnexin-disrupted yeast, and result in the same growth rate as in wild-type yeast.
11

Diminished expression of adenosine kinase in diabetic rats

84%
Sakowicz M., Pawelczyk T.
Cellular and Molecular Biology Letters
|
1999
|
tom 04
|
nr 3
12

Expression levels of sex hormone receptors in brains ofJapanese medaka, Oryzias latipes (Actinopterygii: Beloniformes: Adrianichthyidae)

67%
Ngamniyom A., Sasayama Y.
Acta Ichthyologica et Piscatoria
|
2011
|
tom 41
|
nr 1
Background. In teleost fishes, the brain is the target organ for sex steroid hormones. The actions of sex steroid hormones are mediated by their receptors and play an important role in the regulation of endocrine function in the brain. Japanese medaka, Oryzias latipes, is a species widely used in many fields of experimental biology, including neurobiology. In this study, we examined the mRNA expression levels of androgen and estrogen receptors in medaka brains. Materials and Methods. The brains of adult fish were separated into three parts (forebrain, midbrain and hindbrain). The expression levels of androgen receptor (AR) and estrogen receptor (ER) β from each part of the brain were determined using a semi-quantitative RT-PCR analysis. Results. AR and ERβ levels in males were higher in the forebrain and midbrain than in the hindbrain. In females, AR and ERβ levels were higher in the forebrain than in the midbrain and hindbrain. AR levels in the forebrain and midbrain of males were higher than those of females. Conversely, there was no difference in ERβ level between males and females. Conclusion. These data on hormone receptors provide the foundation for understanding the molecular basis of AR and ERβ mRNA expression levels in medaka brains. In addition, our results suggest that, in Japanese medaka, AR, but not ERβ, expression may exhibit sexual dimorphisms between males and females in the forebrains and midbrains.
13

Analysis of expression of AIF and PARP-1 in cancerogenesis of HPV-positive cervix

67%
Warowicka A., Kwasniewska A., Sobieraj M., Gozdzicka-Jozefiak A.
Bulletin of the Veterinary Institute in Puławy
|
2011
|
tom 55
|
nr 3
The mRNA expression of AIF and PARP-1 in HPV negative non-tumour epithelial cells and HPV-positive cervical cancer cells using the real-time PCR method was examined. An increased level of AIF and PARP-1 mRNA in cervical cancer cells in comparison to normal epithelial cells was demonstrated. These results suggest that changes in the mRNA expression level of AIF and PARP-1 might be involved in cervical cancer development. The analysis of these two factors may represent a new molecular tool for cervical cancer prevention in women with HPV persistent infection.
14

The effects of velvet antler polypeptides on the phenotype and related biological indicators of osteoarthritic rabbit chondrocytes

67%
Zhang Z., Liu X., Duan L., Li X., Zhang Y., Zhou Q.
Acta Biochimica Polonica
|
2011
|
tom 58
|
nr 3
Objective: To study the effects of velvet antler polypeptides (VAPs) on osteoarthritic chondrocytes (OCs) in rabbits. Methods: An osteoarthritic rabbit model was established according to Hulth's method. OCs were isolated and cultured for observation of the cell cycle. Cell proliferation was detected by MTT assay and the cell cycle was monitored by flow cytometry. The phenotype was determined by toluidine blue staining as well as immunohistochemical staining for collagen type II. The expression of MMP-1, MMP-3, MMP-13, TIMP-1, and collagen I and X mRNA by chondrocytes was assayed by RT-PCR. Results: The VAPs had no obvious proliferative effect on OCs and did not affect the cell cycle. However, they significantly reduced the proportion of early apoptotic cells in a dose-dependent manner. Further, VAPs inhibited the expression of collagen I and X mRNA and induced abnormal expression of MMP-1 and MMP-13 mRNA. VAPs had no significant effect on MMP-3 and TIMP-1 mRNA levels. The toluidine blue and collagen type II immunohistochemical staining intensities of VAP-treated chondrocytes were positively correlated with the concentration of VAPs used. Conclusion: VAPs had no significant effect on OC proliferation and the cell cycle, but did increase the glycosaminoglycan (GAG) and collagen type II expression levels in the extracellular matrix, and down-regulated collagen I and X mRNA expression. Treatment of cartilage cells with VAPs maintained their normal phenotype, inhibited matrix metalloproteinases (MMPs) secretion, kept the balance of cartilage matrix metabolism, and sustained an external environment where the cartilage cells could survive. Moreover, VAPs reduced the proportion of early apoptotic cells, suggesting that they may block the apoptotic pathway in OCs.
15

Effect of total or partial uterus extirpation on uterus-projecting neurons in porcine inferior mesenteric ganglion. B. Changes in expression of neuropeptide Y, galanin, vasoactive intestinal polypeptide, pituitary adenylate-cyclase activating peptide, somatostatin and substance P

67%
Wasowicz K.
Polish Journal of Veterinary Sciences
|
2003
|
tom 06
|
nr 2
16

Expression level of adenosine kinase in rat tissues. Lack of phosphate effect on the enzyme activity

67%
Sakowicz M., Grden M., Pawelczyk T.
Acta Biochimica Polonica
|
2001
|
tom 48
|
nr 3
In this report we describe cloning and expression of rat adenosine kinase (AK) in Esccherichaia coli cells as a fusion protein with 6xHis. The recombinant protein was purified and polyclonal antibodies to AK were generated in rabbits. Immunoblot anal­ysis of extracts obtained from various rat tissues revealed two protein bands reactive with anti-AK IgG. The apparent molecular mass of these bands was 48 and 38 kDa in rat kidney, liver, spleen, brain, and lung. In heart and muscle the proteins that react with AK antibodies have the molecular masses of 48 and 40.5 kDa. In order to assess the relative AK mRNA level in rat tissues we used the multiplex PCR technique with β-actin mRNA as a reference. We found the highest level of AK mRNA in the liver, which decreased in the order kidney > spleen > lung > heart > brain > muscle. Measure­ment of AK activity in cytosolic fractions of rat tissues showed the highest activity in the liver (0.58 U/g), which decreased in the order kidney > spleen > lung > brain > heart > skeletal muscle. Kinetic studies on recombinant AK as well as on AK in the cytosolic fraction of various rat tissues showed that this enzyme is not affected by phosphate ions. The data presented indicate that in the rat tissues investigated at least two isoforms of adenosine kinase are expressed, and that the expression of the AK gene appears to have some degree of tissue specificity.
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