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1

In vitro expression analysis of R68G and R68S mutations in phenylalanine hydroxylase gene

100%
Zekanowski C., Perez B., Desviat L. R., Wiszniewski W., Ugarte M.
Acta Biochimica Polonica
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2000
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tom 47
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nr 2
Phenylketonuria (PKU), an autosomal recessive disorder caused be a deficiency of hepatic phenylalanine hydroxylase (PAH), is clinically very heterogeneous. At the molecular level, more than 400 mutations in the PAH gene are known to date, which in different genotype combinations could account for biochemical and clinical variability of symptoms. In vitro expression studies on R68G and R68S mutations causing mild phenylketonuria are presented.
2
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Molecular cloning and preliminary expression analysis of complete cDNA homologue LOX2 gene in Lupinus luteus

84%
Wilmowicz E., Kucko A., Frankowski K., Kesy J., Marciniak K., Glazinska P., Banach M., Wojciechowski W., Kopcewicz J., Tretyn A.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
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2013
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tom 94
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nr 3
3

Molecular cloning, characterization and expression analysis of PtrHOS1, a novel gene of cold responses from trifoliate orange [Poncirus trifoliata (L.) Raf.]

84%
Liu D.-C., He L.-G., Wang H.-L., Xu M., Sun Z.-H.
Acta Physiologiae Plantarum
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2010
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tom 32
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nr 2
High expression of osmotically responsive genes 1 (HOS1) encodes an ubiquitin E3 ligase that promotes the degradation of transcription factor Inducer of CBF Expression 1 (ICE1). Inactivation of ICE1 reduces CBF-induced activation of many cold-responsive genes, and thus, HOS1 act as a negative regulator of cold-responsive genes. In this paper, a novel HOS1 gene, designated PtrHOS1 (Genebank accession number FJ844367), was cloned by RT-PCR and RACE-PCR from trifoliate orange [Poncirus trifoliata (L.) Raf.]. The full length of PtrHOS1 is 3,434 bp with an open reading frame of 2,922 bp, encoding a protein of 974 amino acids with a molecular weight of 110.2 kDa and a theoretical isoelectric point of 5.55. Sequence alignment showed that PtrHOS1 protein had a conserved RING finger domain in its N-terminal region and shared high identity with other plant species HOS1-like proteins. Semi-quantitative RT-PCR analysis revealed that PtrHOS1 could be constitutively expressed at high levels in leaves, stems and roots. Interestingly, the PtrHOS1 expression had a declined period in leaves, stems and roots after cold and ABA treatments, which suggested that the PtrHOS1 expression was down regulated both by cold and ABA. Moreover, the decline was first occurred in leaves (30 min), followed with stems (2 h) and roots (4 h) after cold treatments. These results probably suggest that the leaves of trifoliate orange first sense the cold stress, followed with stems and roots. Oppositely, after ABA treatments, the significant decline of PtrHOS1 expression was first occurred in roots (15 min), followed with stems and leaves (30 min). Our results provide useful information for further studies about cold acclimation mechanism in citrus.
4
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Identification and expression analysis of a novel phytocystatin in developing and germinating seeds of triticale (x Triticosecale Wittm.)

84%
Siminska J., Bielawski W.
Acta Societatis Botanicorum Poloniae
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2015
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tom 84
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nr 1
In this paper the complete cDNA sequence of a newly identified triticale phytocystatin, TrcC-7, was analyzed. Because TrcC-7 transcripts were present in seeds, we hypothesized that it may regulate storage protein accumulation and degradation. Therefore, changes in mRNA and protein levels during the entire period of seed development and germination were examined. Expression of TrcC-7 increased during development and decreased at the end of maturation and subsequently increased during seed germination. Based on these results, TrcC-7 likely regulates cysteine proteinase activity during the accumulation and mobilization of storage proteins.
5
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Transcriptome signature of the lactation process, identified by meta-analysis of microarray and RNA-Seq data

84%
Farhadian M., Rafat S. A., Hasanpur K., Ebrahimie E.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
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2018
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tom 99
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nr 2
6
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Comparative transcriptomic analyses of four Phalaenopsis species to identify and characterize the WUSCHEL -related homeobox (WOX) gene family

84%
Kanchan M., Sembi J. K.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
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2020
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tom 101
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nr 4
7

Characterization and expression analysis of the yellow lupin [Lupinus luteus L.] gene coding for nodule specific proline-rich protein

84%
Karlowski W. M., Strozycki P. M., Legocki A. B.
Acta Biochimica Polonica
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2000
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tom 47
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nr 2
The LlPRP2 gene coding for a proline-rich protein shows a high level of similarity to, as well as significant differences from the family of ENOD2 nodule-specific genes. Several sequence motifs with putative regulatory function were identified in the 5' and 3' noncoding regions of the LlPRP2 gene. Northern blot analysis revealed that the expression of the LlPRP2 gene begins 9 days after inoculation of yellow lupin roots with Bradyrhizobium sp. (Lupinus); the expression is restricted to symbiotic nodules and is not detected in other tissues or organs. Detailed hybridization analysis showed that, when expression is activated, the LlPRP2 transcript is modified so as to produce at least three bands and a continuous distribution of decay intermediates. The modification of the LlPRP2 transcript probably involves degradation from the 5'- and/or 3'-ends of the RNA molecules. Southern blot analysis indicates that only one gene is present in the yellow lupin genome. The presence of genes homologous to the LlPRP2 gene was confirmed for three cultivars of yellow lupin and for Lupinus angustifolius. However, LlPRP2 homologues were not detected in Lupinus albus cv. Bac, indicating that this plant may lack the ENOD2 sequence.
8
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Molecular cloning and expression analysis of ABA 8'-hydroxylase genes during germination of triticale (X Triticosecale Wittm.) seeds

67%
Fidler J., Zdunek-Zastocka E., Bielawski W.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
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2013
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tom 94
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nr 3
9
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Analysis of expression profiles of barley, medicago and soybean galactinol synthase through microarray data integration

67%
Paukszto L., Myszczynski K., Gojlo E., Jastrzebski J. P., Pupel P., Podlinski P.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
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2013
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tom 94
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nr 3
10

Molecular cloning and expression analysis of a new gene for short-chain dehydrogenase-reductase 9

67%
Liu S., Huang C., Li D., Ren W., Zhang H., Qi M., Li X., Yu L.
Acta Biochimica Polonica
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2007
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tom 54
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nr 1
We report here the cloning and characterization of a novel human short-chain dehydrogenases/ reductase gene SCDR9, isolated from a human liver cDNA library, and mapped to 4q22.1 by browsing the UCSC genomic database. SCDR9 containing an ORF with a length of 900 bp, encoding a protein with a signal peptide sequence and an adh_short domain. GFP localization shows SCDR9 protein concentrated in some site of the cytoplasm, but not in the ER. Expression pattern in eighteen tissues revealed that SCDR9 is expressed highly in liver. Soluble recombinant protein was successfully purified from Escherichia coli using pET28A(+) expression vector. Our data provides important information for further study of the function of the SCDR9 gene and its products.
11

Molecular cloning and characterisation of phosphoglycerate kinase from Fasciola hepatica

51%
Jaros S., Wesolowska A., Jaros D., Zygner W., Wedrychowicz H.
Bulletin of the Veterinary Institute in Puławy
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2011
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tom 55
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nr 3
This paper is focused on cloning and bioinformatical as well as immunological characterisation of the new vaccine antigen candidate against fasciolosis - Fasciola hepatica phosphoglycerate kinase (FhPGK). The antigen was cloned from the adult fluke by the use of RACE-PCR method. It was found that FhPGK is not a secretory and not a stage specific protein. It is present in all kinds of parasite tissue, particularly in fluke intestine and tegumental as well as subtegumental layers. FhPGK is involved in production of the first ATP molecule in the glycolytic pathway and can be used in vaccination trials in which the strategy is to block fluke's energy metabolism. This is the first, to date, phosphoglycerate kinase cloned from F. hepatica.
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