Wyniki wyszukiwania - AGRO

1

Differentiation strategies of alternative splicing variants mRNA estrogen receptor-alpha

100%

Witek A., Mazurek U., Paul M., Wilczok T.

Folia Histochemica et Cytobiologica. Supplement

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2001

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tom 39

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nr 1

2

Proliferating cell nuclear antigen in coumestrol treated human endometrial stromal cells culture

88%

Tomaszewski J., Semczuk A., Miturski R., Jakowicki J.

Cellular and Molecular Biology Letters

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2000

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tom 05

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nr 2

3

Usefulness of immature golden hamster (Mesocricetus auratus) as a model for uterotrophic assay

75%

Radko L., Minta M., Jasik A., Stypula-Trebas S., Zmudzki J.

Bulletin of the Veterinary Institute in Puławy

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2015

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tom 59

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nr 4

The present study assessed the sensitivity of immature hamster uterotrophic assay to reference oestrogen agonists/antagonists in order to develop a sensitive model for evaluation of endocrine-active compounds in diets. After performing a baseline for control animals, the sensitivity of immature females (postnatal day 18) to reference compounds was evaluated in a three-day uterotrophic assay. The absolute and adjusted dry uterine weights, fold induction over control for absolute wet uterine weight, and wet uterine weight/body weight ratio (%) were used as endpoints. The significantly active doses for reference oestrogens were as follows: 0.6 µg/kg for 17α-ethinyloestradiol (s.c.): 1 µg/kg/day (s.c.) and 40 µg/kg (p.o.) for diethylstilboestrol; 40 mg/kg (s.c.) and 160 mg/kg (p.o.) for bisphenol A. Co-treatment with tamoxifen at a dose of 1 mg/kg significantly antagonised the uterotrophic effect induced by 1 µg/kg 17α-ethinyloestradiol, and showed the attenuated proliferative effect in histopathological examination. We found immature hamster uterotrophic assay as a sensitive model that could be a good alternative to the rat assay.

4

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Health risk of exposure to bisphenol A (BPA)

75%

Konieczna A., Rutkowska A., Rachon D.

Roczniki Państwowego Zakładu Higieny

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2015

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tom 66

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nr 1

Bisphenol A (BPA) belongs to chemicals that are produced in large quantities worldwide. It is commonly used as monomer in polycarbonate synthesis, plasticizer in the production of epoxy resins, as well as an additive for the elimination of surfeit of hydrochloric acid during the polyvinyl chloride (PVC) production. BPA is not only used in the production of plastics intended to a direct contact with food, including plastic packaging and kitchenware, but also in inner coatings of cans and jar caps. There are various routes of human exposure to this substance such as oral, by inhalation and transdermal. The main sources of exposure to BPA include food packaging and dust, dental materials, healthcare equipment, thermal paper, toys and articles for children and infants. BPA is metabolized in the liver to form bisphenol A glucuronide and mostly in this form is excreted with urine. Due to its phenolic structure BPA has been shown to interact with estrogen receptors and to act as agonist or antagonist via estrogen receptor (ER) dependent signalling pathways. Therefore, BPA has been shown to play a role in the pathogenesis of several endocrine disorders including female and male infertility, precocious puberty, hormone dependent tumours such as breast and prostate cancer and several metabolic disorders including polycystic ovary syndrome (PCOS). Because of the constant, daily exposure and its tendency to bio-accumulation, BPA seems to require special attention such as biomonitoring. This observation should include clinical tests of BPA concentration in the urine, which is not only one of the best methods of evaluation of the exposure to this compound, but also the dependence of the daily intake of BPA and the risk of some endocrine disorders.

5

Immunohistochemical localisation of oestrogen and epidermal growth factor receptors of the bitch uterus in the sexual cycle

75%

Ozyurtlu N., Sagsoz H., Saruhan B. G., Zonturlu A. K., Ketani M. A., Akbalik M. E.

Bulletin of the Veterinary Institute in Puławy

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2010

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tom 54

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nr 2

193-200

The localisation of oestrogen (ER) and epidermal growth factor receptors (EGFR) in the various cell types of the bitch uterine was determined. In this study, 23 adult, healthy crossbred bitches brought to the clinic for ovariohisterectomy were used. ER and EGFR positive staining was detected in all cell types of the uterus. A distinct staining was seen in the luminal and glandular epithelium; while stromal and myometrial cells showed weak or moderate staining. The endothelial and smooth muscle cells of the vessels in the endometrium and myometrium sometimes appeared positive. No staining was observed in the mesothelium. The results of this study suggested that ER and EGFR were expressed at various levels in different cell types of bitch uterus. In light of the previous studies, and data of the presented investigations, it may be necesssary to elicit the harmonious proliferation and differentiation of epithelial and stromal cells that are considered essential for the preparation of the uterus for implantation.

6

Effects of estrone on quisqualate-induced toxicity in primary cultures of rat cortical neurons

75%

Kajta M., Marszal M., Kubera M., Lason W.

Journal of Physiology and Pharmacology

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2005

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tom 56

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nr 2

Estrogens exert protective effects against neurotoxic changes induced by over-activation of ionotrophic glutamate receptors, whereas little is known about their interaction with changes mediated by metabotropic glutamate receptors. We evaluated effects of estrone on quisqualate (QA)-induced toxicity in neuronal cell cultures on 7 and 12 day in vitro (DIV). Twenty four hour exposure to QA (150 µM and 300 µM) significantly decreased cell survival in 7 day old cultures, but the 12 day old cultures were more resistant to its toxicity. DNQX (10 µM), an AMPA/kainate receptor antagonist, partly attenuated the toxic effects of QA, whereas LY 367 385 (100 µM), a selective mGluR1alpha antagonist, completely reversed the above effect. QA did not activate, but suppressed spontaneous caspase-3-like activity. Estrone (100 nM and 500 nM) attenuated QA-mediated neurotoxic effects independently of estrogen receptors, as indicated with ICI 182, 780 and without affecting the caspase-3-like activity. At early stage of development in vitro (7 DIV) toxic effects of QA were more profound and mediated mainly by metabotropic glutamate receptors of group I, whereas later (12 DIV) they were mediated mostly by ionotropic AMPA/kainate receptors. The toxic effects of QA were partly accompanied by anti-apoptotic action against spontaneous caspase-3-like activity, possibly due to modulation of neuronal plasticity.

7

Influence of raloxifene and 17beta-oestradiol on rats' oral mucosal structure

75%

Rahnama M., Swiatkowski W., Lancut M., Wojcik A.

Bulletin of the Veterinary Institute in Puławy

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2004

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tom 48

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nr 3

8

Bcl-2 expression in relation to progesterone and estrogen receptors status in endometrial adenocarcinoma in women

75%

Semczuk A., Miturski R., Gogacz M., Tomaszewski J., Jakowicki J. A.

Cellular and Molecular Biology Letters

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1997

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tom 02

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nr 2

9

Estrogen receptors in sheep ovarian follicle development

75%

Tomanek M., Pisselet C., Monget P., Madigou T., Thieulant M. L., Monniaux D.

Folia Histochemica et Cytobiologica. Supplement

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1997

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tom 35

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nr 2

10

An analysis of the expression of estrogen and progesteron receptors and of the proliferative antigens, PCNA and Ki67, in uterine myoma cells as related to the phase of the menstrual cycle

75%

Zaslawski R., Surowiak P., Dziegiel P., Zabel M.

Cellular and Molecular Biology Letters

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2002

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tom 07

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nr Suppl.

11

Morphological changes in endothelial cells of uterine artery of cycling and ovariectomized-estradiol treated ewes

75%

Zezula-Szpyra A., Gawronska B., Skipor J.

Folia Morphologica

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1996

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tom 55

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nr 4

12

Removal of natural estrogens and synthetic compounds considered to be endocrine disrupting substances [EDs] by coagulation and nanofiltration

75%

Bodzek M., Dudziak M.

Polish Journal of Environmental Studies

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2006

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tom 15

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nr 1

Our paper presents the evaluation of retention coefficient of such natural estrogens as estrone (E1), estradiol (E2) and estriol (E3) as well as synthetic compounds considered to be endocrine disrupting substances (EDs) – ethynylestradiol (EE2), mestranol (MeEE2) and diethylstilbestrol (DES) in water treatment processes, i.e. coagulation and nanofiltration. Estrogens were retained in effect of the adsorption on the particles of humic acids and on floccules of Al(OH)₃ and Fe(OH)₃. The efficiency of coagulation process was not good enough to remove these compounds completely from water. As a second stage of water treatment, nanofiltration was proposed. In this process, estrogens were adsorbed on membranes in their pores (Jv/Jw<1), and their retention was dependent on hydrophobility of micropollutants (logKow). The best treatment results were obtained for polyamide membrane (NF-DS5DK) within the range 93.5-99.9%.

13

Molecular basis of gynaecological malignancies: endometrial cancer

75%

Pirog E. C.

Bulletin of the Polish Academy of Sciences. Biological Sciences

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1996

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tom 44

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nr 1-2

14

Morphological changes of the accessory male sexual glands of rats treated with estrogens during neonatal period

75%

Otulakowski B., Limanowski A., Miskowiak B.

Folia Morphologica

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1996

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tom 55

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nr 4

15

Immunohistochemical localization of androgen receptor [AR] in rat ovary

75%

Slomczynska M., Szoltys M.

Folia Histochemica et Cytobiologica

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1997

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tom 35

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nr 2

16

Gonadotropins and sex hormones modulate interrenal function in soft-shelled turtle

75%

Ray P. P., Maiti B. R.

Folia Biologica

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2002

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tom 50

|

nr 3-4

17

Anti- and prooxidative properties of hormonal therapy

75%

Stetkiewicz T., Makowski M., Pertynski T.

Acta Toxicologica

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2007

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tom 15

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nr 1

18

Influence of oleoyl-estrone treatment on circulating testosterone. Role of 17beta-hydroxysteroid dehydrogenase isoenzymes

63%

Romero M. M., Vila R., Fernandez-Lopez J. A., Esteve M., Alemany M.

Journal of Physiology and Pharmacology

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2009

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tom 60

|

nr 1

181-190

Overweight male rats received oral oleoyl-estrone (OE) for 10 days, and were compared with controls. The expression of 17ß-hydroxysteroid dehydrogenase (17ßHSDH) isoenzymes, and other proteins related to sex hormone metabolism, were analyzed in testicle, liver, adrenals and two white adipose sites: subcutaneous inguinal and epididymal pads using a semiquantitative RT-PCR method. Androstenedione, testosterone, estrone and estradiol levels were measured by HPLC-MS/MS. Isoenzyme expressions were grouped according to their main physiological function (oxidative or reductive) and preferred substrate (androgen or estrogen). As expected, testicle was the main site for synthesis of testosterone and estradiol, and the liver the main organ oxidizing them to androstenedione and estrone. Overall oxidative capacity was 6.5-fold higher than the reductive, and estradiol synthesis and oxidation potential were higher than for testosterone. OE decreased serum androgens, and increased estrone, but not estradiol. This was due to decreased testicle ability to produce testosterone, because of smaller size and decreased 17ßHSDH3 expression, but also to lower availability of precursors. High estrone availability (from OE hydrolysis) does not translate into higher estradiol because of decreased testicle reductive 17ßHSDH expression and decreased aromatase. In consequence, we can assume that OE effects on androgens, and the hypothalamic-pituitary-gonadal axis are limited to testicles.

19

The promoter(s) of the aromatase gene in male testicular cells

63%

Carreau S., Bourguiba S., Lambard S., Silandre D., Delalande C.

Reproductive Biology

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2004

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tom 04

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nr 1

20

The presence and role of progesterone receptor in the ovaries of postmenopausal women who have not applied hormone replacement therapy

63%

Starczewski A., Brodowska A., Laszczynska M., Piasecka M.

Folia Histochemica et Cytobiologica

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2008

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tom 46

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nr 3

277-282

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