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The cytochrome P-450 dependent monooxygenase system is responsible for metabolism of exo- and endogenous compounds. It is induced or inhibited by many factors, including hormones. The effects of hormones on this system depend on the dose, manner and route of administration. The aim of this study was to test a controlled long-acting system of estradiol-progesterone release from a biodegradable copolymer and to evaluate the influence of the copolymer alone, or the copolymer with estradiol or progesterone on the hepatic enzymatic system responsible for biotransformation of xenobiotics. We have shown that the copolymer slightly affects the activity of the cytrochrome P-450 - dependent mixed oxygenase system (MFO), but not the desaturase system. Estradiol and progesterone modified the MFO activity in a typical manner.
This study investigated an influence of oestradiol and progesterone on expression of LH/hCG receptors in the porcine uterine broad ligament. The tissues were obtained from nineteen ovariectomized crossbred gilts. The animals received i.m. injections of 2 ml of corn oil (control; n=4), 25 μg kg-1 of oestradiol benzoate (n=5), 500 μg kg-1 of progesterone (n=5) or 25 μg kg-1 of oestradiol benzoate with 500 μg kg-1 of progesterone (n=5) daily for 5 consecutive days before slaughter. It was found that oestradiol increased the number of receptors in arteries of all areas in the uterine broad ligament, in veins located near the uterine horn and in the ovarian vein as well as in the ligament musculature in the area of vessels located near uterine horn. Progesterone decreased the expression of receptors in all tissues from the area of vessels located about 4 cm below the uterine horn, but augmented their expression in the ovarian vein and in muscular layers of the broad ligament in the area of the uterine vessels trunk. Oestradiol with progesterone increased amount of receptors in arteries from the area of uterine vessels branches, in ovarian vessels^ in veins from areas of branches and trunk of uterine vessels as well as in muscular layers of the broad ligament collected from areas of uterine vessels trunk and paraovarian plexus. These results indicate that oestradiol and progesterone are responsible for changes in expression and distribution of LH/hCG receptors in the broad ligament of the porcine uterus.
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