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Origanum dubium is a valuable wild oregano species of the natural flora of Antalya, Turkey. In this study, we extracted essential oil (EO) by hydro-distillation of the aerial parts of selected O. dubium chemotype with highest EO content, and analyzed by gas chromatography/mass spectrometry. Carvacrol was the primary component (85.9%) among 24 different compounds in the EO. The volatile test showed minimum inhibitory effect of the EO against Xanthomonas axonopodis pv. vesicatoria, Clavibacter michiganensis subsp. michiganensis, Xanthomonas axonopodis pv. phaseoli, Xanthomonas axonopodis pv. phaseoli var. fuscans, and Pseudomonas syringae pv. tomato, at 309, 303, 318, 254, and 901 µL/mL, respectively. A 1-hour treatment of bean and tomato seeds in the volatile phase of the EO effectively sterilized the seeds from bacterial pathogens without inhibiting their germination. To the best of our knowledge, this is the first study on the potential for the use of O. dubium EO as a seed protectant against bean and tomato seed-borne bacterial pathogens.
Borytis cinerea is responsible for the most postharvest losses of table grape. In the current research, the effect of essential oils extracted from sweet basil, fennel, summer savory and thyme plants were investigated both on mycelial growth of B. cinerea under in vitro condition and on fungal decay and quality sensors of table grape [Vitis vinifera (L.) cv. Tabarzeh] under in vivo conditions. Results showed that essential oils especially thyme, fennel and summer savory oils had a high inhibitory effect on mycelial growth of B. cinerea. Under in vivo assays, thyme and summer savory oils were able to reduce fungal decay sensory on table grape after 60 days of storage. In addition, essential oils had significant efficacy on quality parameters of fruit reducing a weight loss, berry shrinkage and berry and rachis browning. Also essential oil treatment increased the maturity index levels in treated fruits in comparison with controls. GC/MS analysis showed that linalool (65.25%), trans-anethole (64.72%), carvacrol (54.14%) and β-ocimene (12.62%) were the main compounds identified in sweet basil, fennel, summer savory and thyme oils, respectively. Results obtained from presented study showed that essential oils especially these one containing more phenolic compounds had a great antifungal activity and could be used as a benefit and safe tool for preservation of table grape.
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