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Characterization of DNA sequences localized 11 to 17.5 kb upstream of the chicken embryonic pi-globin gene

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Pietrowska M., Rusin M., Widlak P., Razin S. V., Rzeszowska-Wolny J.

Acta Biochimica Polonica

1999

tom 46

nr 3

We have analyzed the DNA fragment localized about 11 to 17.5 kb upstream of the chicken α-globin gene domain (the fragment was designed as α-0). The nucleotide sequence of its 3.3 kb-long 5' part was established and interactions with nuclear matrix proteins were studied. The DNA region localized about 16 kb upstream of the embryonic π-globin gene showed high affinity to nuclear matrices in vitro. Two palindromes and a cluster of inverted repeats were co-localized in the same region. The whole 6.6 kb α-0 fragment decreased the activity of CAT reporter gene when transfected into chicken erythroblastoid cells.

Ograniczanie wyników

1 Acta Biochimica Polonica

1 Pietrowska M.

1 Razin S. V.

1 Rusin M.

1 Rzeszowska-Wolny J.

1 Widlak P.

1 1999

Wyniki wyszukiwania

Characterization of DNA sequences localized 11 to 17.5 kb upstream of the chicken embryonic pi-globin gene