Wyniki wyszukiwania

1

Transforming growth factors (TGFs) produced by R-XC cells

100%

Klein A., Branny J.

Folia Histochemica et Cytobiologica

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1985

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tom 23

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nr 4

2

Specific, irreversible inhibitors of the epidermal growth factor receptor [EGFR] family of tyrosine kinases

100%

Fry D. W.

Cellular and Molecular Biology Letters

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1998

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tom 03

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nr 3

3

Gastric mucin secretion in response to beta-adrenergic G protein-coupled receptor activation is mediated by SRC kinase-dependent epidermal growth factor receptor transactivation

100%

Slomiany B. L., Slomiany A.

Journal of Physiology and Pharmacology

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2005

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tom 56

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nr 2

In many systems, the integration of converging regulatory signals that relay on G protein-coupled receptor (GPCR) activation into functional cellular pathways requires the involvement of receptor tyrosine kinase. In this report, we provide evidence that activation of GPCR by ß-adrenergic agonist leading to stimulation in gastric mucin secretion requires epidermal growth factor receptor (EGFR) participation. Using [3H]glucosamine-labeled gastric mucosal cells, we show that stimulatory effect of ß-adrenergic agonist, isoproterenol, on mucin secretion was inhibited by EGFR kinase inhibitor, PD153035, as well as wortmannin, a specific inhibitor of PI3K. Both inhibitors, moreover, blunted the mucin secretory responses to ß-adrenergic agonist-generated second messenger, cAMP as well as adenylate cyclase activator, forskolin. The gastric mucin secretory responses to isoproterenol, furthermore, were inhibited by PP2, a selective inhibitor of tyrosine kinase Src responsible for ligand-independent EGFR autophosphorylation, but not by ERK inhibitor, PD98059. The inhibition of ERK, moreover, did not cause attenuation in mucin secretion in response to cAMP and forskolin. The findings underline the role of EGFR as a convergence point in gastric mucin secretion triggered by ß-adrenergic GPCR activation, and demonstrate the requirement for Src kinase in EGFR transactivation.

4

Epithelial–mesenchymal transition in chronic rhinosinusitis: differences revealed between epithelial cells from nasal polyps and inferior turbinates

86%

Konnecke M., Burmeister M., Pries R., Boscke R., Bruchhage K.-L., Ungefroren H., Klimek L., Wollenberg B.

Archivum Immunologiae et Therapiae Experimentalis

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2017

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tom 65

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nr 2

5

Monoclonal and bispecific antibodies as novel therapeutics

86%

Booy E. P., Johar D., Maddika S., Pirzada H., Sahib M. M., Gehrke I., Loewen S., Louis S. F., Kadkhoda K., Mowat M., Los M.

Archivum Immunologiae et Therapiae Experimentalis

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2006

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tom 54

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nr 2

6

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Epidermal growth factor [EGF] expression in human salivary glands. An immunohistochemical study

86%

Dubiel B., Mytar B., Tarnawski A., Zembala M., Stachura J.

Journal of Physiology and Pharmacology

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1992

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tom 43

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nr 1

Epidermal growth factor (EGF) is a biologically active peptide involved in differentiation, growth, regeneration and repair of human and animal tissues. Quantitative biochemical studies showed in man the highest concentration of EGF in the parotid gland. The aim of the present study was to define EGF immunolocalization in the individual segments of the human major salivary glands (salivon). The material consisted of sections obtained from the surgically removed salivary glands: parotid, submaxillary and sublingual. Immunohistochemical studies were performed by PAP method using monoclonal antibody against human epidermal growth factor. EGF expression was found almost exclusively in the efferent pathways of the salivary glands, mostly in the intercalated ducts and Pflüger salivary tubules. These segments of the salivon are most developed in the parotid gland in which the staining was stronger than in other salivary glands.

7

The effect of concomitant stimulation with cholecystokinin and epidermal growth factor on extracellular signal-regulated kinase (ERK) activity in pancreatic acinar cells

86%

Daniluk J., Dabrowski A.

Journal of Physiology and Pharmacology

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2007

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tom 58

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nr 3

The transmission of extracellular proliferation and differentiation signals into their intracellular targets is mediated by a signaling cascade culminating in mitogen-activated protein kinase (MAPK) also known as ERK. In pancreatic acinar cells both cholecystokinin (CCK) and epidermal growth factor (EGF) are known to stimulate ERK. Regulatory interactions among individual receptor-coupled signaling cascades are critically important for establishing cellular responses in the face of multiple stimuli. The aim of our study was to evaluate the effect of concomitant stimulation of G protein-coupled receptors (GPCR) and EGF receptors on ERK activity in isolated pancreatic acinar cells. ERK activity was determined by means of Western-blotting, with the use of the antibody which recognizes active, tyrosine-phosphorylated kinase (pY-ERK). pY-ERK level was strongly elevated by 10 nM CCK-8, 100 µM carbachol (CAR), or 100 nM EGF. The addition of EGF to 60 min-lasting incubations of acini with CCK-8 or CAR caused abrupt decrease of pY-ERK level to 56 and 59% of control, respectively. Similar phenomenon was observed when short stimulation with CCK-8 or CAR was superimposed on the effect of EGF. After the addition of EGF to acini incubated previously with phorbol ester TPA, strong decrease in pY-ERK level was also observed. In conclusion, in pancreatic acinar cells, concomitant stimulation with CCK or CAR and EGF has strong inhibitory effect on ERK cascade. This inhibitory cross-talk may be mediated, at least partially, by protein kinase C (PKC). These mutual inhibitory interactions demonstrate novel mechanism for integration of multiple signals generated by activation of G-protein-coupled and growth factor receptors in pancreatic acinar cells.

8

Evaluation of mouse preimplantation embryos cultured in media enriched with insulin-like growth factors I and II, epidermal growth factor and tumour necrosis factor alpha

86%

Kurzawa R., Glabowski W., Wenda-Rozewicka L.

Folia Histochemica et Cytobiologica

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2001

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tom 39

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nr 3

9

Effect of epidermal growth factor [EGF] on Tetrahymena pyriformis

86%

Kohidai L., Keresztesi M., Csaba G.

Acta Protozoologica

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2001

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tom 40

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nr 3

10

Cloning and expression of the hEGF gene in Saccharomyces cerevisiae

86%

Topczewska J., Bolewska K.

Acta Biochimica Polonica

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1993

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tom 40

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nr 1

11

Pathophysiology of reflux oesophagitis and peptic ulcer

86%

Gabryelewicz A.

Journal of Physiology and Pharmacology. Supplement

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1993

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tom 44

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nr 1

12

The epidermal growth factor-like domain from tissue plasminogen activator. Cloning in E.coli, purification and ESR studies of its interaction with human blood platelets

86%

Pietrucha T., Stec W. J., Okruszek A., Uznanski B., Koziolkiewicz M., Wilk A., Plucienniczak A., Swiatkowska M., Cierniewski C. S.

Acta Biochimica Polonica

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1994

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tom 41

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nr 1

To examine whether the epidermal growth factor (EGF)-like domain Pr047-Asps7 is involved in the interaction of tissue plasminogen activator (t-PA) with platelets, we have expressed this domain in £. coli. The peptide fragment was produced from a plasmid expression vector as a fusion protein with P*galactosidase Meti-Valm at high yield in eight clones of E. coli. The fusion protein was purified and subjected to mild acid hydrolysis with formic acid, then the peptide Pro47-Asps7, identified by immunoblotting using specific antibodies to t-PA, was isolated by HPLC. After incubation with blood platelets spin labelled with 16-doxylstearic acid or 5-doxylstearic acid, the Pro47-Asps7 peptide fragment reduced fluidity of the membrane lipid bilayer to the same extent as did intact t-PA as indicated by ESR measurements. Our data suggest that the EGF-like domain of t-PA can directly interact with blood platelets and thus it seems to contain those sites of the t-PA molecule that bind the platelet membrane components.

13

Prognostic significance of epidermal growth factor receptor in surgically treated squamous cell lung cancer patients

86%

Niemiec J., Kolodziejski L., Dyczek S., Gasinska A.

Folia Histochemica et Cytobiologica

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2004

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tom 42

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nr 2

14

Exocrine gland morphogenesis: insights into the role of amphiregulin from development to disease

72%

Sisto M., Lorusso L., Ingravallo G., Lisi S.

Archivum Immunologiae et Therapiae Experimentalis

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2017

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tom 65

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nr 6

15

Role of polyamines in gastroprotection induced by epidermal growth factor

72%

Brzozowski T., Drozdowicz D., Majka J., Polonczyk-Pytko J., Konturek S. J.

Journal of Physiology and Pharmacology

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1991

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tom 42

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nr 2

Polyamines have been shown to stimulate cellular growth and differentiation, though their role in the prevention of acute gastric lesion induced by various noxious agents has been little studied. Epidermal growth factor (EGF) exhibits gastroprotective and ulcer healing properties due to its potent mitogenic and growth promoting action. This study was designed to compare the gastroprotective effects of spermine and EGF against gastric damage induced by absolute ethanol, acidified aspirin and stress and to determine the role of endogenous polyamines in EGF-induced gast- troprotection. Spermine and EGF significantly reduced the lesions induced by all three ulcerogens. Oral administration of spermine or subcutaneous infusion of EGF in 24 h fasted rats with chronic gastric fistula resulted in similar inhibition of gastric acid and pepsin secretion. Pretreatment with difluoromethylornithine (DFMO), an irreversible inhibitor of ornithine decarboxylase (ODC), a hey enzyme in the biosynthesis of polyamines, did not affect ethanol lesions, but reversed the protective effect EGF but not spermine, against ethanol. This finding indicates that polyamines mediate, at least in part, EGF-induced gastroprotection. In tests with oral administration of aminoguanidine that is known to suppress the activity of diamino-oxidase (DAO) and to inhibit the degradation of polyamines, EGF showed a markedly enhanced gastroprotective activity against ethanol damage. Since indomethacin failed to affect the gastroprotective effects of spermine and EOF and neither of these agents influenced the mucosal generation of PGE₂ in intact or injured gastric mucosa, we conclude that prostaglandins are not the major factors in spermine- and EGF-induced gastroprotection. This study demonstrates that polyamines are highly effective against gastric lesions induced by various ulcerogens and that they act as primary mediators of EGF-induced gastroprotection.

16

The expression and localization of neuregulin-1 (Nrg1) in the gastrointestinal system of the rhesus monkey

72%

Zhao W.-J.

Folia Histochemica et Cytobiologica

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2013

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tom 51

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nr 1

17

Differential expression of epidermal growth factor and transforming growth factor beta isoforms in dog endometrium during different periods of the estrus cycle

72%

Bukowska D., Kempisty B., Jackowska M., Wozna M., Antosik P., Piotrowska H., Jaskowski J. M.

Polish Journal of Veterinary Sciences

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2011

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tom 14

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nr 2

Both epidermal growth factor (EGF) and transforming growth factor (TGF) play an important physiological role in the processes of proliferation and differentiation of several different cell types. However, the expression profiles of these factors in domestic bitches endometrium are still poorly recognized. The aim of the present study was to identify and analyze the differential expression of these factors in various stages of the estrus cycle. Endometrial tissue from proestrus (n=17), estrus (n=10), day 10 diestrus (n=15), day 35 diestrus (n=18) and anestrus (n=25) was collected soon after ovariohysterectomy. Total RNA was isolated from the endometrium by means of Chomczyński and Sacchi method, treated by DNase I, and reverse- transcribed into cDNA. Quantitative analysis of EGF, TGFβ1, TGFβ2, and TGFβ3 cDNA was performed by real-time quantitative polymerase chain reaction (RT-PCR). EGF expression in canine endometrium was increased in the estrus stage as compared to proestrus (P<0.05), day 10 diestrus (P<0.05), day 35 diestrus (P<0.01) and anestrus (P<0.001). We also found the differences in EGF expression between day 10 and day 35 of estrus as well as between day 35 of estrus with anestrus (P<0.05, P<0.01, respectively). The TGFf1 transcript contents were also higher in estrus as compared to other stages (P<0.01). The TGFβ2 and TGFβ3 in the estrus stage was increased compared to proestrus, day 10 diestrus, day 35 diestrus and anestrus (P<0.05). We proved that expression of EGF and TGFβ transcript isoforms is related to the phase of estrus in bitches and therefore may be regulated by specific hormone concentrations during these periods. Our results confirm the hypothesis that these growth factors play a role in the regulation of biochemical changes in the endometrial tissues during the estrus cycle.

18

Nerve growth factor injected into the gastric ulcer base incorporates into endothelial, neuronal, glial and epithelial cells: implications for angiogenesis, mucosal regeneration and ulcer healing

72%

Tanigawa T., Ahluwalia A., Watanabe T., Arakawa T., Tarnawski A. S.

Journal of Physiology and Pharmacology

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2015

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tom 66

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nr 4

19

The effect of tyrphostins AG494 and AG1478 on the autocrine growth regulation of A549 and DU145 cells

72%

Bojko A., Reichert K., Adamczyk A., Ligeza J., Ligeza J., Klein A.

Folia Histochemica et Cytobiologica

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2012

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tom 50

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nr 2

20

Helicobacter pylori infection and expressions of EGF, EGFR and c-erbB-2 proteins in gastric carcinoma

72%

Pryczynicz A., Guzinska-Ustymowicz K., Kemona A., Czyzewska J.

Folia Histochemica et Cytobiologica

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2009

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tom 47

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nr 3

Ograniczanie wyników

Transforming growth factors (TGFs) produced by R-XC cells

Specific, irreversible inhibitors of the epidermal growth factor receptor [EGFR] family of tyrosine kinases

Gastric mucin secretion in response to beta-adrenergic G protein-coupled receptor activation is mediated by SRC kinase-dependent epidermal growth factor receptor transactivation

Epithelial–mesenchymal transition in chronic rhinosinusitis: differences revealed between epithelial cells from nasal polyps and inferior turbinates

Monoclonal and bispecific antibodies as novel therapeutics

Epidermal growth factor [EGF] expression in human salivary glands. An immunohistochemical study

The effect of concomitant stimulation with cholecystokinin and epidermal growth factor on extracellular signal-regulated kinase (ERK) activity in pancreatic acinar cells

Evaluation of mouse preimplantation embryos cultured in media enriched with insulin-like growth factors I and II, epidermal growth factor and tumour necrosis factor alpha

Effect of epidermal growth factor [EGF] on Tetrahymena pyriformis